EMAGE mouse gene expression spatial database

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EMAGE:7079

Acpl2 acid phosphatase-like 2 ( MGI:2442444)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002477. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002477_01	euxassay_002477_02	euxassay_002477_03	euxassay_002477_04

euxassay_002477_05	euxassay_002477_06	euxassay_002477_07	euxassay_002477_08	euxassay_002477_09

euxassay_002477_10	euxassay_002477_11	euxassay_002477_12	euxassay_002477_13	euxassay_002477_14

euxassay_002477_15	euxassay_002477_16	euxassay_002477_17	euxassay_002477_18	euxassay_002477_19

euxassay_002477_20	euxassay_002477_21	euxassay_002477_22	euxassay_002477_23	euxassay_002477_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7079_wholemount_strong.wlz

7079_wholemount_moderate.wlz

7079_wholemount_weak.wlz

7079_wholemount_possible.wlz

7079_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7079_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 21 22 23 24 moderate expression: see section 18 19 20

pectoral girdle and thoracic body wall musculature

strong

regional

strong expression: see section 12 13

brain

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

spinal cord

weak

regional

weak expression: see section 11 12 13 14 15

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 16 17 18 19

meckel's cartilage

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22 23

lower jaw molar

strong

regional

strong expression: see section 05 06 07 08 09 17 18 19 20 21

upper jaw molar

strong

regional

strong expression: see section 05 06 07 08 09 17 18 19 20 21

nucleus pulposus

moderate

regional

moderate expression: see section 08 09 10 11 16 17 18

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5091
Entity Detected:	Acpl2, acid phosphatase-like 2 ( MGI:2442444)
Sequence:	sense strand is shown >T5091 CCACGCGTCCGCTGGCTGCGGGTGGAGATCCCTACGCACAGCGACGGAGGCCGGGACCGTAGGCGGATGG AAACCCTTGGGCAAGGCTAGACACAGCAGTTGAGCTGAAGTCTCACCATGGAAGAAGAACCACAAATGTG GCCACAGATTTGGGTGCCTTCCCAGATGGCTAGAGATGTGGACCAGTGAACAGGGACGTGCCCAGTGGCC AGGATTGTGAGAAGAAAGTACTGCATGAAGCAGGAGCCACAGTTCTGGAGAGAGAGTGAATCCTTGTGCT TAGGACGGGTTCCCGATTGGAGGTGAAACCATGAAGAGAACGTAGGCCACACAACAATGCTTCACCGTAA TCGCTTCTTGGTGCTGCTGGCCCTGGCTGGGCTCTTGGCTTTCCTGAGCCTCAGCCTGCAGTTCTTCCAC TTGATCCCAGTGTCTGCCACAAAGAATGGAGGAAGCAGCAAGAGTCGAAAGAGGATCATGCCTGACCCAG TGACAGAGCCTCCCACCGTAGACCCGGTTTATGAAGCTCTTCTGTACTGCAACATTCCAAGCGTAGCGGA GCACAGCATGGAAGGTCATGCCCCGCATCATTATAAGCTGGTCTCCGTTCATGTGTTCATTCGCCATGGG GACAGGTACCCACTGTATGCCATTCCCAAAACAAAGCGGCCAGAAATCGACTGCACTCTAGTGGCTAGCA GGAAGCCGTATCACCCTAAACTAGAAGCTTTCATTAGTCACATGTTGAAAGGATCCGGAGCCTCTTTTGA AAGCCCCTTAAATTCCCTGCCTCTCTATCCTAACCATCCTCTGTGTGAGACGGGAGAGCTCACGCAGACA GGAGTCGTGCAGCATCTGCTGAATGGCCAGCTGCTGAGGGACATCTATCTGAGGAAACACAAACTTCTGC CCAACAACTGGTCCTCAGACCAGCTTTACCTGGAGAGCACGGGGAAGAGCCGCACCCTGCAGAGCGGGCT AGCCCTGCTCTACGGCTTCCTCCCAGAGTTTGACTGGAAGAAGGTTTATTTCAAGCACCAGCCAAGTGCC CTGTTCTGCTCTGGAAGCTGCTACTGCCCGCTGAGAAACCAGTATCTGGAGAAGGAACAGCGACGCCAGT ACCTGCTACGTTTGAAGAACAGCGACCTAGAGAGGACCTACGGGGAGATGGCCAAGATTGTGGACATCCC CACGAAGCAGCTCCGCGCGGCCAACCCCATCGATTCCATGCTCTGCCACTTCTGCCACAATGTCAGCTTC CCCTGCAGCAGAAGCGGCTGCCTTGGCATGGAGCACTTCAAGGTGATCAAGACGCACCAGATAGAGGATG AGAGGGAGCGCCACGAGAAGCTCCTGTACTTTGGGTACTCCCTCCTCGGGGCCCACCCCATCCTAAATCA GACGGTCAACCGGATGCAGCGCGCTGCCTCGGGCTGGAGGGATGAGCTGTTCACCCTCTACTCTGCTCAT GACGTCACCCTGTCACCCATCCTCAGTGCCTTGGGTCTTTTGGAAGCCAGGTTCCCGAGGTTTGCCGCCA GGCTGGTTTTTGAGCTCTGGCAAGACCGTCAAAAGCCCAGTGAACATTCGGTCCGAATTCTTTATAACGG GGCTGATGTTACCTTCCACACCTCCTTCTGCCATGACTTCCACAAGCGCTCTCCCAAGCCTATGTGTCCT CTTGAGAACTTGGTCCGCTTTGTCAAACGGGATATGTTTGTGGCCCTGGATGGCAGTAGTACTAACTATT ACGATGCCTGTCATGGGGAAGGGGCCTAAGCGGTGTTCAGCACAGCAGTGTTTTATTGCTGGTTCTGTCT GTTGCTAAGATGTCAAGGTATGGAGTTCTAATGGATGCAGATTAGGTGGGGTCTTCATGCTCATTGGTTG AGACAGACAGGAAGCACATAACAGGATGTTACTTGGTACAAAATAGCCGGTTCACAGAGGAAAGAAGGTA CGTTTTCATGGCCGTGTGCAGCTTAGAGTGCCAGCATAAGGCTCAGTACTCAACAGCCAACCTTATATAA CTTTTGACCTGCCATGGAACCAGCAAACCTCTGCCAAAAATCTTCTTAATCTCCGACATTTACCTTAACC TCGTCGAGTGGAAAATTTTCCAGAGTGATGAGTTCACAGTGTGCTTGGCTTGGCAGCCTTTCTCTGGGGG CGGGGACCAGAAGGTCAGTATCTTAGGCTCTGTAGACCTAAAGGTCACAGAGAATCTCTGTCATATCTTC TCAGCTGTGCTCTGGTGGTGTGGCGACGCATAAGTGGCCATGTGTGAGCAAACAGAGTGAGTGGCAGCCA GCTCGGGCTCTCTGGCCGTGTCTGCTGACTCATCTTTAAAAGCAGCCTGAACCGCTGTGCTTCCAGCACT TTGAAGGCCAAACGACCGCTGTTCTTCAGGTCCTTCAGTAACGTCTGCTAGAAGCACAGCGTTGGGTCTG TATCTGACGTGGTAACACGCCCAGGGGAACGAACACCAGGTCAGAAAGCACGCAGGAAATTACTACAGTA CTTGATGCTCGTGAGGATCGTGGCACAAAATAGCTTTAAGTGTGTCTTAGATACTTGTCTGCCGTAGTCT ATTGGCTGCATATGCTGGAATTTTTGTATTCCATTTAGTATTTTTATAGTCTAGGGAAATATTTTCTGAG GCCAGTTTTAGATGTGTTTGGATTCCTATAGAAATACTCGGTTTACTGCACCTGCTTGGTGGTTGGAAGG AGGCCAAAGCTCAATTCAGACACTCGTTTTCCCTCCAAAAATAAATAATGACCTGTTCCCTTTGACAAGC TGTAGAACTGGGTCCATTTTTAAACCATTTTCATCAATTTCAAATGCCAATTTCCAATTGATTTTTAAAG AGGTTTGTAGAAAGAAAAAAAGAACAACTTTGTTACTCCATAGTTAAATAATCTTTCTAAAGTGTTTTCT ATATTAGGCGTGATTGCAGCTAACGCCGTGGTTCCTGGGTTAACGGTGCTTGTTCGTTCCGCAGACATGG CCCATGCAGGTGGGTTCTCCAGCTTCAGCATTCCAGCCTTTCTCTTCCTTTGTGTCTGGTGTTGCACTCG AACACGTTTTGTTTTTAGAAATAAACATTTTGGAATTCTTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5323284 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5323284 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7080 same embryo
	EMAGE:7078 same embryo
	EMAGE:7082 same embryo
	EMAGE:7081 same embryo
	EurExpress:euxassay_002477 same experiment
	MGI:4822935 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source