EMAGE mouse gene expression spatial database

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EMAGE:7081

Lime1 Lck interacting transmembrane adaptor 1 ( MGI:1919949)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002601. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002601_01	euxassay_002601_02	euxassay_002601_03	euxassay_002601_04

euxassay_002601_05	euxassay_002601_06	euxassay_002601_07	euxassay_002601_08	euxassay_002601_09

euxassay_002601_10	euxassay_002601_11	euxassay_002601_12	euxassay_002601_13	euxassay_002601_14

euxassay_002601_15	euxassay_002601_16	euxassay_002601_17	euxassay_002601_18	euxassay_002601_19

euxassay_002601_20	euxassay_002601_21	euxassay_002601_22	euxassay_002601_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7081_wholemount_strong.wlz

7081_wholemount_moderate.wlz

7081_wholemount_weak.wlz

7081_wholemount_possible.wlz

7081_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7081_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 01 02 moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

trigeminal v ganglion

moderate

regional

moderate expression: see section 09 20 21 22 weak expression: see section 04 05 06 07 08 18 19

spinal cord

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15 16

cervico-thoracic ganglion

weak

regional

weak expression: see section 10 17

cervical ganglion

weak

regional

weak expression: see section 09 10 18

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5237
Entity Detected:	Lime1, Lck interacting transmembrane adaptor 1 ( MGI:1919949)
Sequence:	sense strand is shown >T5237 GTCCGCTCCAGTTATACACCCTTGTTCAGTTGAGGTCATAGCTACCCTGGCCATGCTCCTCTATCTCCAT CTCCATTCCTGATAACTAACAGGGATAGGCAGAGCACTTCTTACCAGGAACCCTAGCTAGAGAGGGAAAG GTGGGTCCCCCTAGAGCACGGCAAAAATGCAAGGCTTGGGGGTTTCCGGTGTGCTGAGTCAACCAGGGAG AAGGAACCCACTAGTGGGCAAGATCTGCAAGTAGGCTGCAGCTGTACACTGCTCTCGCAGCCAGCCCCCA GTGCTCATCTCTTCTGTCCCCAGGGCTCTCTTCCCTGTTTCACAGGATGAGGCCACCAGTGCCTTCAGCC CCACTAGCTCTCTGGGTCCTGGGCTGCTTCTCCCTGCTCCTTTGGCTATGGGCACTGTGCACAGCCTGCC ACAGGAAGAGAGCTCAGAGGCAGCAAACTGGGCTGCAGGACAGTTTGGTACCAGTGGAAATGCCACTGCT GAGACAGACCCACCTCTGCTCCCTCAGCAAGTCTGACACCAGACTGCATGAGCTGCACCGAGGCCCACGC TCCAGCATAGCCCCACGGCCTGCTAGCATGGATCTTCTGCATCCACGCTGGCTAGAGATGTCCAGGGGCA GCACCAGGTCTCAGGTACCCAACTCTGCCTTCCCACCACGGCAGCTGCCCAGGGCCCCTCCTGCTGCTCC TGCAACTGCACCTTCTACTAGCTCTGAGGCCACTTATTCCAATGTGGGGCTGGCTGCAATTCCCAGGGCC AGCCTGGCTGCTAGCCCTGTGGTGTGGGCTGGAACACAGCTGACCATTAGTTGTGCCAGGCTTGGTCCTG GGGCCGAATATGCCTGCATCCAGAAGCACAAAGGGACAGAGCAAGGCTGCCAAGAATTGCAGCAGAAGGC TAAGGTGATCCCAGCTACTCAGATGGACGTCCTATACTCTAGGGTTTGCAAGCCTAAAAGAAGAGACCCA AGACCCGTCACAGACCAGCTGAACCTGCAGGATGGGAGAACAAGTCTGCCGCTTGGGAGTGATGTGGAGT ACGAGGCCATCAATCTCAGGGGCCAGGATATGAAGCAAGGGCCCTTAGAAAATGTGTATGAAAGCATCAA GGAGATGGGCCTGTGAGCAGCTCAGGCCTTACATCCTGTCTTTAACATGAGTTAGGAAGAGTACCAAGGC TTCTGCTTCAGGATGGTTTGATTGCAGATCCTTCTGTGTCCTTCCACCTCCACAGCTGGCAGCACAGCTA TCAGGGCCAGTCCTTGAGGCCTAATTAAACCTCTGTTCAATAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5357176 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5357176 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7080 same embryo
	EMAGE:7079 same embryo
	EMAGE:7078 same embryo
	EMAGE:7082 same embryo
	EurExpress:euxassay_002601 same experiment
	MGI:4825927 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source