EMAGE mouse gene expression spatial database

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EMAGE:7089

Zfp52 zinc finger protein 52 ( MGI:99199)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002612. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002612_01	euxassay_002612_02	euxassay_002612_03	euxassay_002612_04

euxassay_002612_05	euxassay_002612_06	euxassay_002612_07	euxassay_002612_08	euxassay_002612_09

euxassay_002612_10	euxassay_002612_11	euxassay_002612_12	euxassay_002612_13	euxassay_002612_14

euxassay_002612_15	euxassay_002612_16	euxassay_002612_17	euxassay_002612_18	euxassay_002612_19

euxassay_002612_20	euxassay_002612_21	euxassay_002612_22	euxassay_002612_23	euxassay_002612_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7089_wholemount_strong.wlz

7089_wholemount_moderate.wlz

7089_wholemount_weak.wlz

7089_wholemount_possible.wlz

7089_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7089_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

strong

homogeneous

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon marginal layer

moderate

regional

moderate expression: see section 07 08 09 18 19 20 21

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 08 09 10 11 14 15 16

hindbrain

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 moderate expression: see section 22 23

midbrain

strong

homogeneous

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21 moderate expression: see section 22

spinal cord

strong

homogeneous

strong expression: see section 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5199
Entity Detected:	Zfp52, zinc finger protein 52 ( MGI:99199)
Sequence:	sense strand is shown >T5199 CCACGCGTCCGTAGGAAGCAAGGTGTGTTAGCTTGGGAAGTGGGACTAAAAAGTCAAAGTCTGGAGTGTC AAGAATCCGGTACAAGACTGAGCTGGGGTTGGGGTTGAGGGGTAGAGCGGCTGCACAGTGTGGAATCGCG TGGTGGAGACCTGAGTATGGAGGAAACGGCGGGGCTCGCAGAACCGGAAAACTTACCTATACCGCAGGGC TCACTGAACACCCAGACCTCAGACCTGCTCACCTGTGAAACATAACAAAGAGCCCTAGAAGGTGGAGAGG GAGGAGACAGTACCTAAGGATCCAGACCACAATCTGGCTATTCATTGTTATTCAAAGAGAAAATCAAAGA AGAGAAACAGGAAGAAATGGCCAATTCTCCAGTAAACACATCCCAGGGTCTGTTGACATTCCGAGATGTG GCTGTGGACTTCTCCCAAGAGGAATGGGAATGCCTGGACTCTGCTCAGAGGGCTTTGTACATTGATGTGA TGGTAGAGAATTATAGCAACCTGGTCTCTGTGGAGAACTACTGCATATGTGATACTGTCCATCAACCTGT GAGGACTGAAAAGGAGTCTTGTCCATGTAATGAGCTCAACGAAATGCTTCATGAACCCTCCAACTGTGCA CTTTATAATAGAAGTGACACTACAGAAGCCTCTAACAACTACAGATGCTGTAATGAGAAGGATGCCTCTG TTGACTCATCAAACGCAGATAGACACAAAAGTATGCACATCGGAGAAGAAGACACTTGCCAATCTAAAGA CTGTGAGAAATCTTTCAATTTGTGTTCCAACATTCGGCAATATCAAAGACTCAACACTGAAAAGAAAGAG CACAAGCAGGATGAACATGATGACCATTTCAGCTCTCTATATAGTCTCATGCAAGAAACAGTTGACATGG AAGAAAAGCCATATCAATGTGGGCACTGCAGGAAATACTTCAGTACCACCTCAAGCCTCAGTATCCATCA GAGAATTCATACTGGAGAGAAACCTTACAAATGCAATATTTGTGGCAAATCCTTTAATCAGTGGTCAAGC CTTAAAACAGATCAAACACTGCATACGGCAAAGAAACCATACAAATGCAAAGAAGGTGGAAAGTTACTTG TGCAGTTATCTGCACTTAAAAGCCATCAGAGAAGGCATACTGGAGAGAAGCCTTACAAATGTCAGCAGTG TGACAGATCCTTTGCCCACTGTGCATCATTAAGATGGCATCAGAAAATTCATTCTCCTGAGATACATTAT GAATGCAAAGAATGTGGTAAGTCCTGTTTTGAATTATCACATCTTAAAAGGCATTGCAGAATCCATACTG CCGAAAAGCCTTACAGATATGAGGTATGTGATACATCTATTACTGTGAATTCAACTCTTAAAACTCATCA TAAAATTCATACCAGAGAGAAACCTTACAGATATAGGGAATGTGACAAATCTATTACCAAGTGCTCATAT CTTAGAACATATCAGAGAGTTCATACTGGAAAGAGACCGTATAGATGTACAGAATGTGACAAATTTTTTA CTAAGCACTCAAATCTTAGACAACATCAGAAAATTCATACTGGAGAGAAACCTCCCAAGTGCAAAGATTG TGATAAATCCTTTCTCCGGATTTCTAGTGTTAGAATACGTCAGAAAATTCATGCTGGAGAGAGACGTTAT CGATGTAAGGAATGTGGCAGATCTTTTACCTGGTTCTCAAATCTTAGAGTCCATCAGCGAGTTCATACTG GAGAGAGACCTTACAGATGTAAGGAATGTGGCAAATCTTTTACTACGGGCTCAAATCTTAGAGTCCATCA GAAAATTCATACTGGAGAGAAACCTTACAAATGCAAAGACTGTGACATATCCTTTCTGTGGATTTCAAAT CTTAGAACACATCAAAAAATTCATACTGGTGAGAAACCTTACAAATGCCAGGAATGTAACAAATCTTTCA CAAGGTGCTCATATCTTAGAGACCATCAGAAAATTCATACTGGAGAGAAACTTTACAAATGCAAAGACTG TGACATATCATTTATGTGGATATCAAGTCTTAGAAGACATCAGAAAATTCATACTGGAGAGAAACCTTAC AAATGTAGAGGATGTGAGAAATCCTTTACTCAGTGTTCAACTCTAAGAGACCATCAGAAAATTCATACTG GAGAGAAACCTTACAAATGCAAAGACTGTGGCATATCCTTTTTGTGGATTTCAAGTCTTAGAAGACATCA GAAAATTCACACTGGAGAGAAACCTTACAAATGCAAAGACTGTGACATATCCTTTCTGTGGATTTCGAAT CTTAGAACACATCAGAAAATTCATACTGGAGAGAAACCTTACAAATGTCAAGAATGTAACAAATCTTTTA CTAGGTGCTCCTATCTTAGAGTCCATCAGAGAACTCATTCTGGAGAGAAACTTTACAAATGCGAAAACTG TGACGTATCCTTTATGTGGATTTCGAATCTGAGAAGACATCAGAAAATTCATACAGGAGGAAAACATTCC CTTGTAAATAATGTGACATAACATATATCTGGTATTCTCTGCTTGCAAATCAAATGAGATAAGTGATTGC AACATAAAGATAACATAACTAGATGGTTAAAGCCTTTAATGAGGATTGAAATCTTACAAAATGAATCAGA GTTTATATTAAAATAAAATTCTGCAAATGTAACTGTGGGGAAAGCTTTTTATTTCTTTTAATATGTATGC CTGGCCTGTAAGTATTTCTGTGTACTACATGTATGCCTGGCGCACGTATAAGCCAAAAGAGAGCATCAGA TCCCCTGAAGTTGGCATTATATACAGTTGTGTGTAGCTGTTTATATGTTGGGAATTGAACTCTTATCCTC TGGAATTGCAGCCAGTGCTCTTAACTACTGAGCCTTCTCTGCCATCCCTGAGAAAAGCTATAACAAAAAC ATATAGCTTATTCAACATCCAACCATTCATAATAATGACAGGTAAAATGGTACATGCCTTTAATCACAGC ACACAGAAGACAGAGATCACTGTGAATCCAGTTCCAGGACTTCCCGAACTACATAGACCCTTTCTCAAAA AAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5353641 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5353641 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7092 same embryo
	EMAGE:7090 same embryo
	EMAGE:7088 same embryo
	EMAGE:7091 same embryo
	EMAGE:7093 same embryo
	EurExpress:euxassay_002612 same experiment
	MGI:4829316 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source