EMAGE mouse gene expression spatial database

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EMAGE:7091

Adpgk ADP-dependent glucokinase ( MGI:1919391)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002586. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002586_01	euxassay_002586_02	euxassay_002586_03	euxassay_002586_04

euxassay_002586_05	euxassay_002586_06	euxassay_002586_07	euxassay_002586_08	euxassay_002586_09

euxassay_002586_10	euxassay_002586_11	euxassay_002586_12	euxassay_002586_13	euxassay_002586_14

euxassay_002586_15	euxassay_002586_16	euxassay_002586_17	euxassay_002586_18	euxassay_002586_19

euxassay_002586_20	euxassay_002586_21	euxassay_002586_22	euxassay_002586_23	euxassay_002586_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7091_wholemount_strong.wlz

7091_wholemount_moderate.wlz

7091_wholemount_weak.wlz

7091_wholemount_possible.wlz

7091_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7091_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

weak

regional

weak expression: see section 04 05 06 07 08 21 22 23 24

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 19 20 21 22 weak expression: see section 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5200
Entity Detected:	Adpgk, ADP-dependent glucokinase ( MGI:1919391)
Sequence:	sense strand is shown >T5200 CCACGCGTCCGAAAGGATCCTGTAGCCACCGGTGCGGCGGTGTGTGCACATGGCGCTGTGGCGCGGCTCT GCGTGCGCCGGCTTCCTGGCGCTGGCCGTGGGCTGCGTGTTCCTGCTGGAGCCGGAGCTGCCGGGCACGG CGCTGCGCTCGCTCTGGAGCTCCCTGAGGCTGGGACCCGCGCCAGTGCCCGTGGGACCTCTGTCCCCGGA GAGCCGGCTGGCAGCCGCTTGGGACGCCCTCATCGCGCAGCCCGCCCGGCGCTGGCGCCGCGTGGCTGTG GGAGTCAACGCCTGTGTGGATGTGGTAATCTCTGGCGTGAAACTCCTGCAGGCCCTCGGCCTCAGTCCTG GGAGTGGAAAAGATCATGCTATTCTACACTCGAGAAGTGATCTGGAAGAAGCCTTCCTCTACTTCATGGG GAAGGGAGCCGCTGCTGAGCGCTTCTTCAGTGATAAAGAGACCTTTCATGACATTGCCCAGGCTGCATCT GAGTTTCCAGGAGCTCAGCACTATGTAGGAGGAAATGCTGCTTTAATTGGACAGAGGTTTGCCGCCAACA CAGACTTAAAGGTTCTTCTTTGTGGTCCAATTGGCCCAAAACTGCATGAACTTCTTGATGACAATGTGTT TGTTCCACCAGAGTCACTGCAGGAAGAGGATGAGTTCCACCTTATTTTAGAGTACCTGGCAGGGGAGGAG TGGGGCCCGTTTAAAGCTCCCCATGCCAACCGCTTCATCTTCTCTCACGACCTCTCCAACGGGGCCATGA ATATGCTGGAGGTGTTTGTGTCTAGCCTGGAGGAGTTCCAGCCTGACCTGGTGGTCCTTTCTGGATTGCA CATGATGGAGGGACAAAGCAAGGAGCTCCAGAGGAAGAGGCTCTTGGAGGTTGTGACCGCCATTTCTGAC ATCCCCACTGGTATTCCAGTTCACCTAGAGCTGGCCAGTATGACCAACAGGGAGCTCATGAGCAGTATTG TGCATCAGGTCTTTCCCGCGGTGGCTTCTCTTGGGCTGAATGAACAGGAGCTGCTGTTTCTCAGCCAGTC GGCATCTGGACCCCATTCTTCTCTCTCCTCCTGGGATGGTGTTCCTGATGTGGGCATGGTCAGTGACATC CTCTTCTGGATCTTGAAAGAACATGGAAGGAGTGAAAACAGATCCTCGGATCTCACCAGGATCCATTTCC ACACGCTGGTCTACCACATTCTGGCAACTGTGGACGGACACTGGGCCAACCAGCTCGCAGCCGTAGCTGC GGGAGCTCGTGTAGCTGGGACACAAGCCTGTGCCACAGAAACCATAGACACCAACCGAGTGTCTCTAAGA GCACCTCAGGAGTTTACGACATCCCATTTGGAATCCGGTTCCAGGATTGTATTGAACCCAGATAAGCCAG TGGTGGAATGGCACAGGGAGGGAATAACTTTCCACTTCACACCTGTGTTGGTGTGTAAAGACCCCGTTCG AACTGTAGGCCTTGGTGATGCCATTTCCGCTGAAGGCCTCTTCTATTCAGAAGCACGCCCTGATTAGACA CGTTGTGTGAGGAATGAAAGTCACCCATCTTAAGAATTCCAGCAAGAACATGGCTTGGGAACTAGGCTTG TGAGTGTCAGAACAGTTCCTAGATCAAATTGGAATAGAGCCAAAGAAATCACAGCAGATTAAACTGTACA GATACATTCCAGCCTGTTGGCAACATAAAAACACTTCAGGTATAATTGAAACTCAGCTGTCTACTAACGA GACTGGATTTTTGTTTTTTATGTTGTGCTACAAAGGTTAAAACCTCTTTCTGAATCCCCAGTTGATGTAC CATGACTCTGGAGAGCTCATCCAGCCACAGAAAGTACTCCCCCCTCCCCCCCCCCCCAATCCTGATCACT CATGAAACCTGGCCAAGGCAGTCATGACAGCTTATGCTCCTGGCAGAATCAACTTGTCCATTCAGTGTTA CAGTGACAATATTTGCTGTGTGTACCTTCTCAGCCTCCTGCTCTCAGACATAGGTCAGAGCCACAGGAGA GAACAAAACAGTCTTGTGCTTTATGGTCTCAGACTGGAAACCCTGGGAGAGCTATGAGCCACCTGACCAT TGTGCACTTGCTTGGAAGCACCATGCCTTCCTGCCCCGGTGTCATGGACTGGGGCTGGCTGAGAAAAGGC CCCACAGCTTGTTCTCAGCCTCAGTGCTGAGCTCCGGAAGTGCTTTCTGCAGCACAAGACTGCTACTCGT TCCTTTTGAGCCTTGAAGAGATGTGTTTTATAACTGGAAAGTGCTTCTATATAGGACACATCATGTATTT TGTGTTTTTGTTTTGACACATCCATTAAAGAGTTTGACCTCTTAAAAGCCCCAGGATCATGAATAAATGT GTCATGTATATTTATTTTTTAATAAATCAAGATTTCTGTGTGCTTTTAAAAATATTAAAATAATTTACAT TTCCAGGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5353637 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5353637 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7092 same embryo
	EMAGE:7090 same embryo
	EMAGE:7088 same embryo
	EMAGE:7093 same embryo
	EMAGE:7089 same embryo
	EurExpress:euxassay_002586 same experiment
	MGI:4823012 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source