EMAGE mouse gene expression spatial database

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EMAGE:7093

D4Bwg0951e DNA segment, Chr 4, Brigham & Women's Genetics 0951 expressed ( MGI:106510)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002594. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002594_01	euxassay_002594_02	euxassay_002594_03	euxassay_002594_04

euxassay_002594_05	euxassay_002594_06	euxassay_002594_07	euxassay_002594_08	euxassay_002594_09

euxassay_002594_10	euxassay_002594_11	euxassay_002594_12	euxassay_002594_13	euxassay_002594_14

euxassay_002594_15	euxassay_002594_16	euxassay_002594_17	euxassay_002594_18	euxassay_002594_19

euxassay_002594_20	euxassay_002594_21	euxassay_002594_22	euxassay_002594_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7093_wholemount_strong.wlz

7093_wholemount_moderate.wlz

7093_wholemount_weak.wlz

7093_wholemount_possible.wlz

7093_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7093_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 weak expression: see section 08 09 10 19 20 21 22 23

hand mesenchyme

strong

regional

strong expression: see section 20 21 22 23

hindlimb digit 1 mesenchyme

strong

regional

strong expression: see section 04 05 17 19 22

hindlimb digit 2 mesenchyme

strong

regional

strong expression: see section 04 05 17 18 19 21 22

hindlimb digit 3 mesenchyme

strong

regional

strong expression: see section 04 05 06 17 18 19 20 21 22 23

hindlimb digit 4 mesenchyme

strong

regional

strong expression: see section 05 06 18 19 20 21 23

hindlimb digit 5 mesenchyme

strong

regional

strong expression: see section 19 20 21

stomach

moderate

regional

moderate expression: see section 04 05 06 07 08 09

meckel's cartilage

moderate

regional

moderate expression: see section 02 03 04 05 06 14 15 weak expression: see section 07 16 17 18 19 20

lower jaw molar

moderate

regional

moderate expression: see section 03 04 05 06 14 15

upper jaw molar

moderate

regional

moderate expression: see section 03 04 05 06 14 15

tail mesenchyme

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5204
Entity Detected:	D4Bwg0951e, DNA segment, Chr 4, Brigham & Women's Genetics 0951 expressed ( MGI:106510)
Sequence:	sense strand is shown >T5204 CCACGCGTCCGCTAAAAGGAAAGGACACTGCTGCAGCAACCAGGAAGCCCTTGAACCCCAGCTAAGCTAA CCGTGTCTTTTTCTTTTTTCTTTCTAAAATGCGCCTACACCGCCTACACACAGGACGAACCCACTTCTTT CATTGATCAGATTTTTTTTTTTTTTTCCTGACCGTCTAAGATAGTCTGATCCTTGGCGACATATGGCTGG AAAATGAAACGACCCGGGAGTGTGGCCACCTCGTTATTCTGTGTGGTGTAATACCAGATGGGCACCGTGA ACCGCGCTGGGATGTGAATGGACAGTTTGAAATGGGAATTTCCCCGCATCGAGCAGGTTCCATTTCTCTC TTGGGTCTCTCGGTATAACGTACTAGCTTTTGCCCTTCTTCTCTGCAGTATCAATTAACTCAACTTGGCG GTGGAGTGGCCAGCTAGGAAAGGAATGAAGCGATCTGGCTAATCTTGCTGGCTGTGGAGCGGCAGAGCCT GAAACTGCAGCCGGGTGCAGATCTGCCGACTGCGCCCGGGGCATGGAGCGCACGGGCGACCGCGGGGACT CAGGCCCGGCCAGAGGAGCCCAGTACTGAGCTCCGGAGTCCGCCAGGCCGCCGCCGCCGCCTCCTCGCGC CCTCTGCCTTTACTGTCAGTGTCGCCCCTCGCAAAGAGTCATGGAAGACAGCCCGCTGCCAGACCTCAGA GACATCGAGCTGAAGCTGGGGCGCAAAGTGCCCGAGAGCCTAGCGCGCTCGCTGCGCGGGGAGGAGCTGG CGCCTCGGGAAGGGGCTGCGGACCCCAGCGGGGTTGGTGGGAGCTGCAGCAGCAGCAGTAGCTGCAGCAG CTTTGCGCCCTCCGTGTCCTCCTCGTCGTCATCCTCCCCTGCCTCTGGCTCTCCACGACGTAGCCACCCC AGCGCCCTGGAGAGGTTAGAGACCAAACTGCACATCCTCAGACAGGAGATGGTTAACCTCAGAGCCACTG ATGTAAGGCTCATGCGTCAATTGCTCCTCATCAATGAGAGCATCGAGTCCATCAAGTGGATGATCGAGGA AAAAGCCACCATTACCAGCCGAGGCAGCAGTCTCAGTGGCAGCCTGTGTAGTTTGCTGGAGAGTCAGAGT ACCTCCTTACGTGGCAGCTACAACAGCCTCCATGATGGCAGTGATGGGCTGGATGGCATCTCAGTAGGGA GCTACCTGGACACTTTGGCAGATGATGTACCAGGTCATCAGACCCCTTCAGACTTGGATCAGTTCAGTGA CAGCTCCATCATAGAGGACTCACAGGCTCTGCACAAGCATCCCAAGTTGGATTCTGAGTACTACTGCTTT GGCTAATGATCCACTTTTTTGCATGGGACTGGTGTGCAATTAAACTTGTATGTATCCTTCTTCTCTGCTG CTATATTTTTGGTGTGATTTATTTTAATAAGGCAACCTTTTTAAAGAAGCTTATTTTTAAACTGCTTAAT GATATTTCTGTTGCTCCTAATATTTCTCATCTAGACTGATTTATTTTTCTCTGTTACATCTCTATTTTTA TTTATTGTAATAATCTCTCTCCCTTCTCTTAAAGTAGCACAGACAAAGTAGAGGGGGAAAATAAGCAATA ATTGTTTCTGCTTTTGATTTCAGAGTAAGGGGTCAGGGATTATATGAACACTTTGCTAAATTTTACAATA AACCAAAGTCTGATCACTTCAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5354204 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5354204 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7092 same embryo
	EMAGE:7090 same embryo
	EMAGE:7088 same embryo
	EMAGE:7091 same embryo
	EMAGE:7089 same embryo
	EurExpress:euxassay_002594 same experiment
	MGI:4824205 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source