EMAGE mouse gene expression spatial database

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EMAGE:7102

C1qbp complement component 1, q subcomponent binding protein ( MGI:1194505)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002685. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002685_01	euxassay_002685_02	euxassay_002685_03	euxassay_002685_04

euxassay_002685_05	euxassay_002685_06	euxassay_002685_07	euxassay_002685_08	euxassay_002685_09

euxassay_002685_10	euxassay_002685_11	euxassay_002685_12	euxassay_002685_13	euxassay_002685_14

euxassay_002685_15	euxassay_002685_16	euxassay_002685_17	euxassay_002685_18	euxassay_002685_19

euxassay_002685_20	euxassay_002685_21	euxassay_002685_22	euxassay_002685_23	euxassay_002685_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7102_wholemount_strong.wlz

7102_wholemount_moderate.wlz

7102_wholemount_weak.wlz

7102_wholemount_possible.wlz

7102_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7102_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

strong

regional

strong expression: see section 02 03 04 05 18 19 20 21

thymus primordium

weak

regional

weak expression: see section 10 11 12 13 14

submandibular gland primordium

strong

regional

strong expression: see section 05 06 07 08 16 17 18 19

vibrissa

moderate

regional

moderate expression: see section 04 05 06 19 20

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 07 08 09 10 15 16 17 18 19 weak expression: see section 02 03 04 05 06 11 12 20 21 22 23

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 13 14 weak expression: see section 12 15

midgut

moderate

regional

moderate expression: see section 10 11 12 13 14 15 17 18

lower jaw incisor

weak

regional

weak expression: see section 10 11 13 14 15

lower jaw molar

weak

regional

weak expression: see section 05 06 07 08 09 16 17 18

upper jaw incisor

weak

regional

weak expression: see section 10 11 14 15

upper jaw molar

weak

regional

weak expression: see section 05 06 07 08 09 16 17 18

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 20 21 moderate expression: see section 19 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5842
Entity Detected:	C1qbp, complement component 1, q subcomponent binding protein ( MGI:1194505)
Sequence:	sense strand is shown >T5842 CCACGCGTCCGACGGTGCCTTGGCCCGGTCGTCCTTCCCTCTGCACGTGTCCGCCCCGCGATGCTCCCTC TGCTGCGTTGCGTGCCCCGCGCCCTCGGCGCCGCCGCCTCGGGCCTCCGAACCGCCATCCCGGCCCAGCC GCTTCGGCATCTCCTGCAGCCCGCGCCCCGGCCATGCCTCCGGCCCTTCGGTTTGCTCAGCGTACGGGCC GGCTCGGCTCGGCGCTCTGGCCTCCTGCAGCCCCCGGTTCCCTGCGCGTGCGGCTGTGGCGCTCTGCACA CGGAAGGAGACAAGGCCTTCGTTGAATTCTTGACTGATGAAATTAAGGAAGAAAAGAAGATCCAGAAACA CAAGTCCCTTCCCAAGATGTCTGGAGATTGGGAGCTGGAGGTGAACGGCACGGAGGCTAAATTATTGCGC AAAGTTGCCGGAGAAAAGATCACGGTCACTTTCAACATCAACAACAGCATCCCTCCAACATTTGATGGTG AGGAGGAGCCCTCACAGGGGCAGAAGGCTGAAGAACAGGAGCCAGAACTGACATCAACTCCCAACTTTGT GGTTGAAGTTACAAAGACTGATGGCAAGAAGACCCTTGTACTGGACTGTCACTATCCTGAGGATGAGATT GGACACGAAGATGAGGCCGAGAGTGATATTTTCTCTATCAAGGAAGTTAGCTTTCAGGCCACTGGTGACT CTGAGTGGAGGGATACAAACTATACACTCAACACAGATTCCCTGGACTGGGCCTTGTATGACCACCTAAT GGATTTCCTTGCGGACCGAGGGGTGGATAACACTTTTGCGGATGAGTTGGTGGAGCTCAGCACAGCCCTG GAGCACCAGGAATATATCACCTTTCTTGAGGACCTCAAAAGCTTTGTCAAGAACCAGTAGAACTCAGAGA CTGCGGGCCTTAATTTAAATGGCAAGCTTTGGCCAGTGAACAAAAGCTCCCTTGGCATCAGAATTATGCT TCAAAAATGGCTGTCATCCTAATATATCGGGGGGAAGCAAGTTTAAATTACTGCTGTTACACCTCCATTC GCTATTCCTTTGGGCTTTTTTTCTCTGTACAAATTTATTATTTGTAGATTTTTGTATAACATGATGATGG ACAATAAATCTGACTCCAATAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3154276 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3154276 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7101 same embryo
	EMAGE:7100 same embryo
	EurExpress:euxassay_002685 same experiment
	MGI:4823534 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source