EMAGE mouse gene expression spatial database

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EMAGE:7142

Kcne1l potassium voltage-gated channel, Isk-related family, member 1-like, pseudogene ( MGI:1913490)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002655. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002655_01	euxassay_002655_02	euxassay_002655_03	euxassay_002655_04

euxassay_002655_05	euxassay_002655_06	euxassay_002655_07	euxassay_002655_08	euxassay_002655_09

euxassay_002655_10	euxassay_002655_11	euxassay_002655_12	euxassay_002655_13	euxassay_002655_14

euxassay_002655_15	euxassay_002655_16	euxassay_002655_17	euxassay_002655_18	euxassay_002655_19

euxassay_002655_20	euxassay_002655_21	euxassay_002655_22	euxassay_002655_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7142_wholemount_strong.wlz

7142_wholemount_moderate.wlz

7142_wholemount_weak.wlz

7142_wholemount_possible.wlz

7142_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7142_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 19 20 21 22 23

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 12

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 11 12

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12

telencephalon ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

medulla oblongata alar plate ventricular layer

moderate

regional

moderate expression: see section 13 14 15 17

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 10 12 13 14 15 17

pons ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 17 18

spinal cord ventricular layer

moderate

regional

moderate expression: see section 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3290
Entity Detected:	Kcne1l, potassium voltage-gated channel, Isk-related family, member 1-like, pseudogene ( MGI:1913490)
Sequence:	sense strand is shown >T3290 TGGCCTCGAGCCAGATTCGGACGAGGTGCACCCTGCCTGCTCTGCTTGCTCCCCTTGCTCGCTTGCTTCC CCTTGTCCAGGGGGAAAGGTCAACTGCGTCCTGGAGCCTCCGTGCCACTCTCCAACAGCTATGAACTGCA GCGAGAGCCAACGGCTGCAAACCCTCTTGAACCGCTTGCTGCTGGAGCTGCACCATCGTGGCAACGCCAG CGGCCTGGGCATCGGAACCGGCCCGAGCATGGGCATGGGGGTCGTCCCTGACCCTTTCGTGGGCCGCGAG GCGACCAGCGCCAAGGGTAACGACGCTTATCTCTACATCCTACTCATCATGATCTTCTATGCCTGCCTAG CCGGAGGCCTCATCCTGGCCTACACCCGCTCCCGCAAGCTCGTCGAGGCCAAGGATGAGCCACCCCTGGC CTGTGTTGCTGAGCAGGAATGGGTCCCCGCTGCCATCGCATCTGCCGACCCGGAGAATGGCCAGGGCTTG CTGGCTGAGGGCGGCCACCAGCTCGCAGCAGGGGCGCTGCCTGCGCTGG
Notes:	The probe template was PCR amplified from IMAGE:2780405 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2780405 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7140 same embryo
	EMAGE:7139 same embryo
	EMAGE:7141 same embryo
	EMAGE:7144 same embryo
	EMAGE:7143 same embryo
	EurExpress:euxassay_002655 same experiment
	MGI:4825706 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source