Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7140

Plin5 perilipin 5 ( MGI:1914218)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7140 EMAGE:7140 EMAGE:7140 EMAGE:7140 EMAGE:7140
"Pseudo-wholemount" of euxassay_002426. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002426_01 euxassay_002426_02 euxassay_002426_03 euxassay_002426_04
EMAGE:7140 EMAGE:7140 EMAGE:7140 EMAGE:7140 EMAGE:7140
euxassay_002426_05 euxassay_002426_06 euxassay_002426_07 euxassay_002426_08 euxassay_002426_09
EMAGE:7140 EMAGE:7140 EMAGE:7140 EMAGE:7140 EMAGE:7140
euxassay_002426_10 euxassay_002426_11 euxassay_002426_12 euxassay_002426_13 euxassay_002426_14
EMAGE:7140 EMAGE:7140 EMAGE:7140 EMAGE:7140 EMAGE:7140
euxassay_002426_15 euxassay_002426_16 euxassay_002426_17 euxassay_002426_18 euxassay_002426_19
EMAGE:7140 EMAGE:7140 EMAGE:7140 EMAGE:7140
euxassay_002426_20 euxassay_002426_21 euxassay_002426_22 euxassay_002426_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7140Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7140_wholemount_strong.wlz
7140_wholemount_moderate.wlz
7140_wholemount_weak.wlz
7140_wholemount_possible.wlz
7140_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7140_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3327
Entity Detected:Plin5, perilipin 5 ( MGI:1914218)
Sequence:sense strand is shown

>T3327
TGGCCTCGAGCCAGATTCGGACGAGGCGGCACGAGGGATGCTGGCCCAGCTGCAGGAAACGCTGGAGCTG
ATCCAGCATATGCAGAGAGGGGCAAGCCCTAGCCCTACTTTCCATCCCCCAAAGACTCAGGAGCTGTGGG
GGAGCTGGAGCCCGTGTCTAGAGAATGGCCGCAGCCACAGTGAGGTGGAGCTGGAGACACTGGCTCTGTC
TCGAAGTTTGACCCTGGAGCTGCAGAATGCAGTGGATGCCCTGGCAGGCTGTGTTCGGGGCCTGCCACCT
AGTGCCCAGGCCAAGGTGGCTGAGGTGCAGCGCAGCGTGGATGCTCTACAGGCCACCTTTGCTGATGCAC
ACTGCCTTGGTGATGTGGCACCCACTGCTCTGGCTGAGGGCCGGGGCAGTGTGGCCCGGGCACATGCCTG
TGTGGATGAGTTCCTGGATTTGGTCCTGCGGGCCATGCCACTGCCCTGGCTTGTGGGGCCCTTTGCACCC
ATCCTGGTGGAACAGTCGGAGCCCCTGATCAACCTGGCCACCTGTGTGGACGAGGTGGTGGGTGACCCTG
ATCCTC
Notes:The probe template was PCR amplified from IMAGE:313143 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:313143 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7139 same embryo
 EMAGE:7141 same embryo
 EMAGE:7144 same embryo
 EMAGE:7143 same embryo
 EMAGE:7142 same embryo
 EurExpress:euxassay_002426 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS