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EMAGE:7155

Pts 6-pyruvoyl-tetrahydropterin synthase ( MGI:1338783)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7155 EMAGE:7155 EMAGE:7155 EMAGE:7155 EMAGE:7155
"Pseudo-wholemount" of euxassay_002610. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002610_01 euxassay_002610_02 euxassay_002610_03 euxassay_002610_04
EMAGE:7155 EMAGE:7155 EMAGE:7155 EMAGE:7155 EMAGE:7155
euxassay_002610_05 euxassay_002610_06 euxassay_002610_07 euxassay_002610_08 euxassay_002610_09
EMAGE:7155 EMAGE:7155 EMAGE:7155 EMAGE:7155 EMAGE:7155
euxassay_002610_10 euxassay_002610_11 euxassay_002610_12 euxassay_002610_13 euxassay_002610_14
EMAGE:7155 EMAGE:7155 EMAGE:7155 EMAGE:7155 EMAGE:7155
euxassay_002610_15 euxassay_002610_16 euxassay_002610_17 euxassay_002610_18 euxassay_002610_19
EMAGE:7155 EMAGE:7155 EMAGE:7155 EMAGE:7155 EMAGE:7155
euxassay_002610_20 euxassay_002610_21 euxassay_002610_22 euxassay_002610_23 euxassay_002610_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7155Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7155_wholemount_strong.wlz
7155_wholemount_moderate.wlz
7155_wholemount_weak.wlz
7155_wholemount_possible.wlz
7155_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7155_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5246
Entity Detected:Pts, 6-pyruvoyl-tetrahydropterin synthase ( MGI:1338783)
Sequence:sense strand is shown

>T5246
AGACCATGAGCGCGGCGGGTGACCTTCGTCGCCGCGCGCGACTGTCGCGCCTCGTGTCCTTCAGCGCGAG
CCACCGGCTGCACAGCCCATCTCTGAGCGATGAAGAGAACTTAAGAGTGTTTGGGAAATGCAACAATCCG
AATGGCCACGGGCACAACTATAAAGTTGTGGTGACAGTCCATGGAGAGATTGATCCTGTTACAGGAATGG
TTATGAATTTGACCGACCTCAAAGAATACATGGAGGAGGCCATCATGAAGCCTCTTGATCACAAGAACCT
GGACCTGGATGTGCCGTACTTTGCGGATGCTGTGAGCACGACAGAAAATGTAGCTGTCTACATCTGGGAA
AGCCTCCAGAAACTTCTTCCAGTGGGAGCTCTTTATAAAGTAAAAGTGTTTGAAACCGACAACAACATCG
TAGTCTATAAAGGAGAATAGGTCTTAGGGTTGCTATTGTAGACAGGCACATTTCTTTTCACACTTGAAAA
AGGCTTTGTCCTTCCAGAGTTCGCAGCAGTCACCACATGCTTGTGTCTCCATGTTCTGTTCTAGAGTACC
CGAGCATTAAAGTAATGTAAGGCCTGTATTTAGATGTATTTACGAATCAAACTTTCAGTGTATCCTGCAT
GTGGTTTTGGTGAGAAGGTAGAGAAGGATTGAGGATTGACACCAGGGCCTCACACGGGAAGCATAGACTC
TTCTGAAGTGCATTCCAGTCCTCTCAAGGCTATTTAGAGGGTCTTTTATCTAGAGGTTTAAAATTATTTC
ATCTTGGGCTGGAGAGATGGCTCAGCCGTTAAGTACACTGACTGTTCTTCCAGAGGTCCTGAGTTCAATT
CCCAGAAACCACATGATGGCTCACAACCATCTATAAAGGGATCTGATGCCCTCTTCTGGCATGTAGTTGT
ACATGCAGACAAAGCATTCCTACATTAAAAAAAATACATAAATAAAATTTAAAAATAATTTCATTTGAGC
AAAATGTTTTGATGTGGAATTATGCAAAGGTAAAATAAACCCATTTTTGTTATCTAAAAAAAAAAAAAAA
A
Notes:The probe template was PCR amplified from IMAGE:5357873 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5357873 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7154 same embryo
 EMAGE:7151 same embryo
 EMAGE:7153 same embryo
 EMAGE:7152 same embryo
 EMAGE:7156 same embryo
 EurExpress:euxassay_002610 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS