EMAGE mouse gene expression spatial database

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EMAGE:7192

Ifi35 interferon-induced protein 35 ( MGI:1917360)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002705. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002705_01	euxassay_002705_02	euxassay_002705_03	euxassay_002705_04

euxassay_002705_05	euxassay_002705_06	euxassay_002705_07	euxassay_002705_08	euxassay_002705_09

euxassay_002705_10	euxassay_002705_11	euxassay_002705_12	euxassay_002705_13	euxassay_002705_14

euxassay_002705_15	euxassay_002705_16	euxassay_002705_17	euxassay_002705_18	euxassay_002705_19

euxassay_002705_20	euxassay_002705_21	euxassay_002705_22	euxassay_002705_23	euxassay_002705_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7192_wholemount_strong.wlz

7192_wholemount_moderate.wlz

7192_wholemount_weak.wlz

7192_wholemount_possible.wlz

7192_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7192_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

bladder

moderate

regional

moderate expression: see section 13 14 weak expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5860
Entity Detected:	Ifi35, interferon-induced protein 35 ( MGI:1917360)
Sequence:	sense strand is shown >T5860 GAGCCACCTCTGAGGGGTCAAAGAGTTTAGTCCTTCAGAACTCTTCACTGCCAACCCTGCTCTGAAGCCC CAGCTTCTGCCAGACGTGAGAGCCATGTCTGTGACCCTGCAAACTGTCCTCTACAGTCTTCAGGAGGAGC AAGCCAGGCTCAAGATGAGGCTGCAGGAGCTGCAGCAGCTCAAAAGGGAGCGCACAGGCTCTCCCGGAGC CAAGATCCCATTCTCAGTACCTGAAGTTCCTCTGGTATTCCAAGGCCAAACTAAGCAGGGCAGGCAAGTG CCCAAGTTTGTAGTTTCTAACTTGAAGGTCTGCTGCCCTCTGCCTGAAGGTTCTGCTCTGGTCACCTTTG AGGACCCCAAAGTGGTTGATCGGTTGCTACAACAAAAGGAACACAGAGTTAACTTAGAGGACTGCTGGCT GCGGGTGCAGGTCCAGCCCTTGGAGCTGCCTGTGGTGACCAACATTCAGGTGTCCAGCCAGCCAGATAAC CACAGGGTGCTCGTTAGTGGTTTTCCTGCTGGACTTAGGCTGAGTGAAGAGGAACTGTTGGACAAGCTGG AGATCTTCTTTGGCAAGGCCAAGAATGGAGGTGGGGATGTAGAGACCCGGGAGATGCTGCAAGGGACCGT CATGCTAGGGTTTGCTGATGAAGAAGTGGCCCAGCACTTATGCCAGATTGGCCAGTTCAGAGTCCCACTG GACCGGCAGCAGGTCCTCCTGAGGGTCTCTCCCTATGTGAGTGGTGAGATCCAGAAAGCCGAGATCAAAT TCCAGCAAGCCCCTCATTCAGTGCTGGTGACAAATATTCCTGATGTCATGGATGCCCAGGAACTGCATGA CATCCTTGAGATCCACTTCCAGAAGCCCACTCGTGGGGGCGGGGAGGTGGAGGCCCTGACAGTTGTGCCT TCAGGGCAGCAGGGCCTGGCTATCTTCACTTCCGAGTCAAGCTAGGCCTGCATGTCCACCTGCTGGGGAC TAGGATCACTTACTGCCTAATGCTACCAAGAACGGTCAAAGTGCCAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3155505 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3155505 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7193 same embryo
	EMAGE:7191 same embryo
	EMAGE:7189 same embryo
	EMAGE:7190 same embryo
	EMAGE:7188 same embryo
	EurExpress:euxassay_002705 same experiment
	MGI:4825515 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source