EMAGE mouse gene expression spatial database

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EMAGE:7202

Dyrk3 dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 ( MGI:1330300)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004406. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004406_01	euxassay_004406_02	euxassay_004406_03	euxassay_004406_04

euxassay_004406_05	euxassay_004406_06	euxassay_004406_07	euxassay_004406_08	euxassay_004406_09

euxassay_004406_10	euxassay_004406_11	euxassay_004406_12	euxassay_004406_13	euxassay_004406_15

euxassay_004406_16	euxassay_004406_17	euxassay_004406_18	euxassay_004406_19	euxassay_004406_20

euxassay_004406_21	euxassay_004406_22	euxassay_004406_23	euxassay_004406_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7202_wholemount_strong.wlz

7202_wholemount_moderate.wlz

7202_wholemount_weak.wlz

7202_wholemount_possible.wlz

7202_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7202_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 09 10 11 19 20 22

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 18 19

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 13 14 17 18

pharynx

moderate

regional

moderate expression: see section 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6410
Entity Detected:	Dyrk3, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 ( MGI:1330300)
Sequence:	sense strand is shown >T6410 AGCGAAGCCAGCAGGTGCGGTGCTGGAGGCGACCCACCGGGCCAACCGCCGCCTCTGTGTCCGCGGGGCG AGCTCGCGATGGGAGGCGCAGCCCGCGATCGCGGGAGGAAGGACGCGGCGCTGCCGGGGGCCGGGCTCCC GCCGCAGCAGCGGAGGTTGGGGGATGGTGTCTATGATACTTTCATGATGATAGATGAAACCAAGGGCCCA CCCTATTCGGACACATTCAGCAACCCCTCTGAAGCACCTGTCTCCAGAAGGCTAAATATTACCACTGAGC CACTCACGAGAGGCCACACTCAGCACTTTGTGAATGGAAGTGAGATGAAGGTGGAACAACTGTTTCAAGA ATTTGGCAACAGAAGATCCAATACTCTCCAGTCAGATGGCATCAGCAACTCGGAAAAGTCTTCTCCGGCT TCTCAGGGGAAAAGTTCAGAAAGCCTGAGCGCGGTGAAGTGCAACCTTTCCTCCAGGCCGTCTAAGGTGC TGCCGCTGACTCCTGAGCAAGCGCTGAAGCAGTATAAACACCACCTCACTGCCTACGAGAAGCTGGAGAT CGTCAGCTACCCAGAAATCTACTTTGTGGGTCCGAATGCCAAAAAGCGGCAGGGAGTTATTGGTGGTCCC AATAACGGTGGCTACGACGATGCGGATGGGGCCTATATTCATGTGCCCCGGGACCATCTGGCTTACCGCT ATGAGGTGCTGAAAATCATCGGCAAGGGGAGTTTTGGACAGGTAGCCCGGGTCTATGATCACAAACTTCG GCAGTACGTGGCCCTGAAAATGGTGCGCAATGAGAAACGCTTCCATCGCCAGGCAGCCGAGGAGATCCGG ATCTTGGAGCATCTTAAAAAGCAAGACAAAACTGGTAGCATGAACGTCATCCACATGCTAGAAAGTTTCA CCTTCCGGAACCACGTGTGCATGGCCTTTGAACTGCTAAGCATAGACCTGTATGAGCTTATTAAAAAAAA CAAGTTCCAGGGCTTCAGCGTCCAGTTGGTTCGGAAGTTCGCCCAGTCCATCCTACAGTCCCTGGACGCT CTGCACAAAAACAAGATCATCCACTGCGACCTGAAGCCGGAAAACATTCTCCTGAAACACCACGGCCGAA GCGCCACCAAGGTCATCGACTTTGGCTCCAGCTGCTTCGAGTATCAGAAGCTTTACACGTATATCCAGTC CCGCTTCTACAGAGCCCCAGAGATCATCCTGGGGTGCCGCTACAGCACCCCGATCGACATATGGAGCTTT GGTTGCATCCTTGCAGAACTTTTGACAGGACAGCCCCTGTTCCCTGGAGAGGACGAAGGAGACCAGTTGG CCTGCATGATAGAGTTGCTAGGAATGCCACCGCAGAAACTTCTGGAGCAATCCAAGCGTGCCAAGTACTT TATTAACTCCAAAGGCTTGCCTCGATACTGCTCCGTATCTACCCAGACGGACGGGAGGGTGGTGCTTCTC GGGGGTCGCTCACGCAGGGGTAAAAAGCGAGGCCCGCCAGGCAGCAAAGACTGGGCAACCGCACTGAAGG GCTGTGGTGACTACTTGTTCATAGAGTTTCTGAAACGATGCCTCCAGTGGGACCCCTCTGCCCGCCTCAC CCCGGCTCAAGCATTAAGACATCCTTGGATTAGCAAGTCTACACCCAAACCTCTCACCATGGACAAGGTG CCAGGGAAGCGGGTAGTTAACCCTACAAATGCTTTCCAGGGACTGGGTTCCAAGCTGCCTCCAGTCGTTG GGATAGCCAGTAAGCTTAAAGCTAACCTAATGTCCGAAACCAGTGGTAGTATACCTCTGTGCAGTGTATT GCCAAAGCTGATTAGCTAGTGGACCACTCAGAGACTGATACATATCATATGTATTTTTAATTACCTTGCA AACATGCAAATGGAAAACGGAATAATTGAAGCCCATTCACTGATGGATATGTTTTTGTTAGACTTTTTTT TAACAAGGCAGAACATTTTTATATGACTATAAAAGAACGCTTCAAGGGCTAATGTCAAACCAGCTTGTAT TGGCCATCTGGAGTATACATTAAATGACTTTTTCATAGGTCAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3584001 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3584001 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7204 same embryo
	EMAGE:7203 same embryo
	EMAGE:7207 same embryo
	EMAGE:7206 same embryo
	EMAGE:7205 same embryo
	EurExpress:euxassay_004406 same experiment
	MGI:4824437 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source