EMAGE mouse gene expression spatial database

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EMAGE:7204

Aldh9a1 aldehyde dehydrogenase 9, subfamily A1 ( MGI:1861622)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004399. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004399_01	euxassay_004399_02	euxassay_004399_03	euxassay_004399_04

euxassay_004399_05	euxassay_004399_06	euxassay_004399_07	euxassay_004399_08	euxassay_004399_09

euxassay_004399_10	euxassay_004399_11	euxassay_004399_12	euxassay_004399_13	euxassay_004399_14

euxassay_004399_15	euxassay_004399_16	euxassay_004399_17	euxassay_004399_18	euxassay_004399_19

euxassay_004399_20	euxassay_004399_21	euxassay_004399_22	euxassay_004399_23	euxassay_004399_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7204_wholemount_strong.wlz

7204_wholemount_moderate.wlz

7204_wholemount_weak.wlz

7204_wholemount_possible.wlz

7204_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7204_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6412
Entity Detected:	Aldh9a1, aldehyde dehydrogenase 9, subfamily A1 ( MGI:1861622)
Sequence:	sense strand is shown >T6412 CGGACGCTTTGCACGCGTAGCAGATGATCCTCGGTGCAGTCGGTTCTGTGCTGACCTCGCTCCTCCGCAT TCACCGGGCTGCAGCAGTCGCCGCCATGAGTACTGGCACCTTCGTCGTGTCGCAGCCGCTCAACTACCGC GGCGGGGCCCGTGTGGAGCCGGTGGATGCTTCCGGCACGGAGAAAGCGTTCGAGCCAGCCACCGGCCGAG TGATTGCCACTTTCGCGTGTTCAGGAGAAAAGGAAGTAAACTTGGCCGTTGAGAATGCAAAGGCTGCCTT TAAACTCTGGAGTAAGAAAAGTGGCCTGGAGCGCTGCCAAGTCCTCCTAGAGGCTGCCCGGATCATCAAG GAGCGGAAGGATGAAATTGCCACCGTGGAGACCATCAACAACGGGAAGTCCATATTTGAGGCCCGCCTGG ATGTTGACACTTGCTGGCAGTGCCTGGAATACTATGCAGGGCTGGCTGCATCCATGGCAGGCGAGCACAT TCAGCTCCCAGGAGGGTCCTTCGGCTACACCCGAAGAGAGCCGCTTGGGGTGTGTGTGGGGATAGGAGCA TGGAACTATCCCTTCCAGATCGCCTGTTGGAAGTCTGCTCCGGCTCTGGCTTGTGGTAATGCCATGATCT TTAAGCCTTCTCCCTTCACGCCTGTGTCTGCTCTGCTCCTGGCTGAGATCTACACCAAAGCGGGTGCGCC TCCGGGGCTCTTCAACGTGGTGCAAGGTGGGGCTGCCACAGGCCAGTTTCTGTGTCATCATCGCGAGGTG GCCAAAATCTCCTTCACCGGAAGTGTGCCCACTGGCGTGAAGATCATGGAGATGTCCGCTAAGGGAGTCA AGCCCATTACTTTGGAACTTGGGGGCAAATCTCCTCTCATCATCTTCTCAGACTGTAATATGGAGAATGC TGTGAAGGGGGCCCTGATGGCCAACTTCCTCACACAAGGACAGGTCTGCTGCAATGGGACTAGAGTCTTT GTGCAAAAGGAAATTGCTGATAAATTCATAAATGAGGTTGTGAAGCAGACTCAGAAGATAAAACTTGGAG ACCCCCTTCTGGAAGATACGAGGATGGGCCCACTCATCAACGCCCCACATCTGGAACGAGTCCTCGGGTT TGTCAAGTTGGCAAAGGAGCAGGGTGCTACTGTGCTGTGTGGTGGAGAGGTGTATGTACCAGAGGACCCC AAATTAAAACATGGATATTACATGACACCTTGCATTTTAACTAATTGCAGAGACGACATGACTTGTGTAA AAGAAGAGATCTTTGGACCAGTCATGTCCATTTTAACATTTGGAACTGAAGCCGAGGTTCTAGAGCGAGC TAATGACACCACTTTTGGACTAGCAGCTGGGGTCTTCACCAGGGACATCCAGAGGGCACACCGGGTGGCG GCTGAGCTGCAGGCTGGAACGTGCTACATTAACAACTATAATGTCAGCCCAGTGGAGCTGCCCTTTGGTG GCTACAAGAAGTCAGGATTTGGCAGAGAGAATGGCCGTGTGACGATTGAGTACTACTCTCAGCTGAAGAC GGTGTGTGTGGAGATGGGCGACGTGGAGTCAGCCTTTTGAAACCCAGGGAAGCCTGCTGGCGTAGCCAAG CTGTGGAATGAGTTGACTGCCTTGACAGGCAGCATAACTGAATAGCTTCTTACATCCAGGCTTTGGGTCA TTAGTGTGATAACGGGTCAGTGTTGTCCTCTGTCTTACAGTCACCTTCCCAGTTTGAAAATGTGCACAAG TGCCTTTCAGATGGTAGCCAGGTAACCTGTTGTCCCAGCCCCTGAGAAATATGTAGACCCCAGAAGCGCA CTCTTCAGACATAGGCAGGCCACAGCTCTGCTCCTACACATTTGTCCCACCAACAATGGCAGCTTTCTAG TTTGCTTTAAAGCAGGCCTTCCTTCCTGGGTTCCTGGTGAATCAGTACATATACATTCTTTGTTCATTGA CAACTAAAAAACAAATTCTTAAAGCTTTTATTTGGAGCTGGGCAGTACTGGTATGTGCCTTTAATCCCGG CACTTGGGAGGCAGAGACAGGTGGATGTCTGAGTTTAGAGCCAGCCTGGTCTACAGAGTGAATTCCAGTC TAGGAAGGTCTACATAGAGAAATCCTGTCTCAAACAAAACAAAACAAAAAAAAACCCATAAATAAATAAA AAAATGTAAAAAGCTGTTATTCTTAGTATTTGTGGGCTATGTGACTCATTGGCATTTAAACAGCTGTGTT CTAACTAACTAGCAAGACAGCTCAGTGGGTAAGGTCTTATCACTTAAACCCAGTGACTTCAGGTTTATCT CTAGAACCCACCCCTTCCCCATATCATACACTGCACAATAATAAAATCTTTCTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3583976 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3583976 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7203 same embryo
	EMAGE:7202 same embryo
	EMAGE:7207 same embryo
	EMAGE:7206 same embryo
	EMAGE:7205 same embryo
	EurExpress:euxassay_004399 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source