EMAGE mouse gene expression spatial database

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EMAGE:7214

Fen1 flap structure specific endonuclease 1 ( MGI:102779)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002717. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002717_01	euxassay_002717_02	euxassay_002717_03	euxassay_002717_04

euxassay_002717_05	euxassay_002717_06	euxassay_002717_07	euxassay_002717_08	euxassay_002717_09

euxassay_002717_10	euxassay_002717_11	euxassay_002717_12	euxassay_002717_13	euxassay_002717_14

euxassay_002717_15	euxassay_002717_16	euxassay_002717_17	euxassay_002717_18	euxassay_002717_19

euxassay_002717_20	euxassay_002717_21	euxassay_002717_22	euxassay_002717_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7214_wholemount_strong.wlz

7214_wholemount_moderate.wlz

7214_wholemount_weak.wlz

7214_wholemount_possible.wlz

7214_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7214_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5905
Entity Detected:	Fen1, flap structure specific endonuclease 1 ( MGI:102779)
Sequence:	sense strand is shown >T5905 GGACTGTCCAGAGAACGCTGTGGACTCCAAACCACTGCTAGCTGCTTAAGGCTCGTGCACTCGAGACGGG GTGAGGTCTCGCGGAAGCGTCTCTGAAAGCGGCAGAGCCGCGGGAACAGCACCGGGCAGCCCGGGCTTGG GCCATTCGCTCTGCTCCGAACATTCCTCTTCGCCGGTAGGAAGAAGCCATTGCTCCTGTGCTACCATGGG AATTCACGGCCTTGCCAAACTAATTGCTGATGTGGCCCCCAGTGCCATCCGTGAGAATGACATCAAGAGC TACTTTGGTCGCAAAGTGGCCATCGATGCCTCCATGAGCATCTACCAGTTCCTGATTGCTGTTCGTCAGG GTGGGGATGTGCTGCAGAACGAGGAGGGTGAGACCACCAGCCACCTGATGGGCATGTTCTACCGTACCAT CCGCATGATGGAGAATGGCATCAAGCCTGTGTACGTCTTTGATGGCAAACCACCACAGCTGAAGTCAGGC GAGCTGGCCAAGCGCAGTGAGAGGCGCGCCGAGGCTGAGAAGCAACTGCAGCAGGCTCAGGAGGCTGGGA TGGAGGAGGAGGTGGAGAAGTTCACCAAGAGGCTCGTGAAGGTCACCAAGCAACACAATGATGAGTGCAA ACACCTGCTGAGCCTCATGGGCATCCCTTACCTTGATGCACCCAGCGAGGCAGAGGCCAGCTGTGCTGCC CTGGCAAAGGCTGGCAAAGTCTATGCTGCGGCCACGGAGGACATGGACTGCCTCACTTTTGGCAGCCCCG TGCTAATGCGACACTTAACTGCCAGTGAGGCCAAGAAGCTGCCCATCCAAGAGTTCCATCTGAGCCGCGT CCTGCAGGAGCTGGGTCTGAACCAGGAGCAGTTTGTGGATCTGTGCATCCTGCTGGGTAGCGACTACTGC GAGAGCATCCGTGGCATTGGGCCCAAGCGGGCTGTGGATCTCATCCAGAAACATAAGAGCATCGAGGAGA TCGTGAGGCGGCTGGACCCCAGCAAGTACCCCGTTCCAGAGAACTGGCTCCACAAGGAAGCCCAGCAGCT CTTCCTGGAGCCAGAAGTACTGGACCCAGAGTCTGTGGAGCTGAAGTGGAGCGAGCCAAATGAAGAAGAG TTGGTCAAATTTATGTGTGGTGAAAAGCAGTTTTCTGAAGAGCGAATTCGCAGTGGGGTCAAGCGGCTGA GTAAGAGCCGCCAGGGCAGCACCCAGGGACGCCTCGATGATTTCTTCAAGGTGACAGGCTCACTCTCCTC AGCTAAGCGCAAGGAGCCAGAACCCAAGGGGCCTGCTAAGAAGAAAGCAAAGACTGGGGGAGCGGGGAAG TTCCGAAGGGGAAAATAAACCTGTCCTTCCCCTCCACTGTCCTTGACCCCAGGCTGTCTATCTGTTTTGT ACCCTCGGCTGCAGCACATCCCTCTTGTCCCTCGTCTTGAGGAGAGTTCATTGCTTCCCAGCGCTGCCCT TCAGAGCTTTCCCTCTCTTGACCCTGTGGCAGGAAGGCCGTAGCTCTGCTTTTTCTCATTTTTAGCTCAG GAAAGATGTCAGGCTCAAACCACTTCTCAGGTTAATGGACACTGTAGTCATTGTTCTGTGCAACTGCGAG CAATGTCTTAAGGAAGAAGAAGATAAAGCCGGGAGCGAGGCTGGAGATAGTTTCCCAGCTGGCCAGCTGG TGGAGGAGAGGTGACTAGAACCTGACTGACTACTGCTCCTTCTAATTTCACTGTGCCCTGAAAGATGCCC ATCAGCCTGGGATTCGCTGATGGAAGAACTGCAAAGAGACGAGCAGAGAGAAGTCTGGCTGACAACAGAT TTAGTACTGACCAGCTGATTTTTGTGGGCAGAAATTTGAACTTGCTGCCTGCTGAGTCCAGTAGTTGTGC AGGGAGTGAGATGGCAGTGTTTAAGTTTTGATTTGTAGTTTTTTGTTTTTGTCTCTCCCCTCTCCAGTGT TGGGGATTGACCCCAGGGCAAAGGCATTAAGTGTGCCACTGACCTGTGCCTCCAAGTGATGTTCTGACAG CCTTTCTGAGGCAATCAATTGAATTGAGGTTTTGGGAGAAGAAACTGTTGTTCATAGGCTATTTCTATTT TAAAAGATGTGAAGAGAAAAAAAAAACAATAAAATTATAAAAGTGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3158176 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3158176 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7215 same embryo
	EMAGE:7217 same embryo
	EMAGE:7218 same embryo
	EMAGE:7219 same embryo
	EMAGE:7216 same embryo
	EurExpress:euxassay_002717 same experiment
	EMAGE:21612 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source