EMAGE mouse gene expression spatial database

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EMAGE:7216

Lynx1 Ly6/neurotoxin 1 ( MGI:1345180)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004333. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004333_01	euxassay_004333_02	euxassay_004333_03	euxassay_004333_04

euxassay_004333_05	euxassay_004333_06	euxassay_004333_07	euxassay_004333_08	euxassay_004333_09

euxassay_004333_10	euxassay_004333_11	euxassay_004333_12	euxassay_004333_13	euxassay_004333_14

euxassay_004333_15	euxassay_004333_16	euxassay_004333_17	euxassay_004333_18	euxassay_004333_19

euxassay_004333_20	euxassay_004333_21	euxassay_004333_22	euxassay_004333_23	euxassay_004333_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7216_wholemount_strong.wlz

7216_wholemount_moderate.wlz

7216_wholemount_weak.wlz

7216_wholemount_possible.wlz

7216_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7216_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

strong

regional

strong expression: see section 06 07 08 09 15 16 17 18

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 12 15 17 19 weak expression: see section 10 16

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 13 14

pons mantle layer

strong

regional

strong expression: see section 08 09 19

ventral grey horn

strong

regional

strong expression: see section 10 11 12 14 15 moderate expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5103
Entity Detected:	Lynx1, Ly6/neurotoxin 1 ( MGI:1345180)
Sequence:	sense strand is shown >T5103 CCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGGCCTACTTGGCCTGCCTGCGATG CGGTACCAACACCGCACGAAGTGTGTACAGATTCCCAGTTAGACAGCAGGAGGGACCTGGGAGCGGCCAG GGGGATGTTTTATCTCTAAGAGACCAAGAGCTCAGGCAGGGCTTCTGTGCCCTGCTTCCTCCCTGGCTTG AGCTGGATCCTGGACCAGCTGCTGACCTCCTGTTCACTCTGGCACTGCCCTCACGTCTCCGTCATGACCC ATCTGCTCACAGTGTTCCTGGTGGCCCTGATGGGCCTGCCTGTGGCCCAGGCTCTGGAGTGCCACGTGTG TGCCTACAATGGAGACAACTGCTTCAAACCCATGCGCTGCCCAGCCATGGCCACCTACTGTATGACCACA CGAACTTACTTCACCCCATACCGGATGAAGGTGAGGAAGTCCTGTGTCCCCAGCTGCTTTGAAACCGTGT ACGATGGCTATTCCAAGCATGCATCTGCCACCTCCTGTTGCCAGTACTACCTCTGCAACGGTGCTGGCTT TGCTACCCCGGTGACCTTGGCCCTGGTCCCAGCACTCCTAGCTACCTTCTGGAGCTTGCTGTAAAGCTCG GTTCCCCAAGCCAGATCCACTCAAACGCAACACTCTCAAAAAACACAGTTTCCCTCTCTCTCCCAATTCA CTCCACCCAACGCTCTTCCTTCTGACACTCCTCAACTACCACGAGGTCCCATGGCTACCTACGAAAGAAC TGATGGCATCCAGATACCTCACTCCAAGGTCATTTTCAGAAGGCTGACATGTGGACCTGTAATGTGCCCA CCCATGGGTGGGGCAGGCTGGGCTTCTCCTCTACCCAAGATCAGGGGCATCTGGGAGAATGTTTATGGAG GAGGGGTCATCACTCAAGTCAAGGAGCACTGATTTGATAGAATTAGTAGCCAAACTCCACCTTCAGAACC CTGCCTCAGTCTACCCAGTAGAGGATGGGTCTGCTAGAGGTGAGGGGAGGAGAGCGGCGGAGAATAACGA GCTGGCTAGAAGCAGAGAAAGACTCAGCAGGGCTGTCTCCGAAGATCAGCGCGGCTTGCCAGAGCAAATG TGATGTGGAAGCCATGTGAGGAAGCCCTTTGTCATTTCCACTTATCTGAGGAACTCTGCCAGACCTGATG TTGGGATAGCCATTGGCCAAGGGTTCCTAGCAACGGCGTCATTTCCATAGGCCACTGAAATCCCTCCAGC CCCAGCTCAGCAGGCCCCTTGACCTCCACTACAGTCCTTCATTCACACACCAGCTGCTGGGCCTTGAAGT TGGCAGGGACTTGGGAGCAGGTGACCCATGCTATTTTTTGTCTGGCCTGTTATTCTGGGCATGGCAAGAA GGGATCAGACGCAGGTCAGAGCAGGGCAGTAGGGCGACTGAGACAGGGAAACAGACTTCAGCCAGTGGCT TCCCAGGTCCCGTAGGCAGCTCCTACATCCTTCAGTCTCTTGTTACATTCCCGGGAGACAAATATACAGG GAGCCAAGCCGAGTGCTAGGTGATGACTGCCTGTGAAGTCTATTGTGGCCACAGACTGCTGGGTACCAAG TCTCAGGAGAACCCAGCCTAGATTTAGGAGACACAGATCTGCCTTTCATGCAGTGTAGCTGTCCTTGGGA GCCTTACCATGCTCTCTAACTAGTACCTCAACTCACATGTCACTGAGGAACCCCCTAACACTGGCCCAGC CCAGGGGTCGGGATGCTGGCCAATGTCCATGGAGTGGGACTACCCATGGAGAGTCCTTGGGTCATCACAT CACAAATGTTTTATTCCAATCTCCCAGTGGTGAGAGCTCGGGACACAAAGGTCCATCCTGGGGACCTTCT TCCTGGTTCTAGGCAGACCTGAACTCTGTCTGCTGCTAGAGCTGATGTGGTTTTCCGCCTCAGTTTCCTC CTCCGGGGATAGGCCACCGGAGGATTTGGGAGGGTGGGGAGGGCATCCTGCTGATGGGCTCGCCGAGGTT CTCAGGAACAGGAACGGGCGGGGCTTTAGTACACAGGTGAGTTGGGTGGGAACTGGCCCGGAGCTGAGGA GACACTGACTGGGCAGAGGGAAGATGAGTCTCAAGGGAGGGCAGGAAAAGGGAGGGGGAGCGCGCATGCA CATGTGCACTCAGTGCAGGCTACAGAGCCCAAAAGGCAGCACTGGCTGTGGTGTCCCCTGAGGCCCAGGC AAGATGCTAGGAGGAAGCCAATGCTGCCCCCACCTGAGCTCACATGGAACATGCACACCACCAGCAGCAG CAGCAAGCATTGAGACTGACCTGTGGACGCCATAGGGCACTGGCAAGGAGGGTCAGAGGCGGGTCCCTGA CTCAGTGGGTGAGGCCCGGGAAACATTATCCTGTTACCCTGCGTGTGCAAGATCATTGTCCCCAGCTAGA TGGCGTCCTCAACCAAAACTGAGAGGAGCCCCAGTTCAGGTCCTCCCTCCTACCACAAGGGGGTGGTGTG GAGGAGGCTTGATTGCCCTTGGAGAAGCACCGGTACTGCAGAGCTGGGGGCCAGCTTCTTTCATCTGTGT CTAGACACCGACCAGATAGGCCCCACAGTGGCAACACTGCCACACAGCCCTACAAGAAGCCCTGTGCCTA GCTAGCACAGAGCCCCAAAAGGTGCTCAATTAATACAGGGCCAAGCCTGCCAGTGGGGGGGATGCAGATT AGGGGAACAGACCCAGATGGCCTGTCCTGAACCCTGTCTGGGGTGGTGTGATGAGCATCTGTCTAGCCCA CTGCAGGTGGCTCTACACACTCCACAACAGTTCTGCAAAAGTGTATGAGGTGGTCATTACTGCGCCCCTC TCACAGGTAAAGGCACTGAGGCACGGAGGAGTGAGGCACTTCATTTTCCTGGGCCATTCAACTTTCCAGG ACCAACACATTCAACTATGGGTACTACTCCAATAGCTGGGGTTCTTTGAGGCTGGGCCCCCTGAAGATGA TAGTGGCTTCATCAACCAGAGAATTTCAGAGTGCAGTGTTGTAGGAGCCTATGAACCTGAAATGTCAGAA CTGGAGGTTTGAGGGGCTGAGGGGTAGGCCAGGGGTGTCTGGCCCCTTGTGTGGAGACAGAGAGAGAGGG AACATGGGATGGGGTAGTAGAGAGAAGTGCAAAGGAGCGTCAGCCTTTCTCAGGGCTAATGCTGTCAGGG ACGAGGGCTCAAGCCTGTGAGTGTTCTCACACTGTGATAAACAGTGGCCCCTCAACACAGACGGTGTCCA GAGTGGCCGGCAGTGGTTATCTAGAGTTGCAATCTGGAAGCCTCTTGGTAGTCACTGGAGAGAGGCCGCT TGATGGGACAGCACCAAATGTGTGTGCTTCTGTGGGATGTGAGGAAGCTGGGTCAGCGCATGAAGCCAAA GCGTCCTTCAGAGCAGAGGGGTGGCTGGTCTAGTCCACCAGAGACAAGCTATCCAGTGAGAGTCATACTC TGCCACCGTCTCTGTGATTACCTTACCCCAAAGCAGACGGGGACGGGATGCAGAGCACCCGTGTCTTCAT CTTCTGCGGCAAGCACGTGAGTTCACATTCTGAAACTCTAGAAAGATTTCCAGGAGTGGGGTGTGCCTTT GCTTTGGTGCATGGTTACTTCCTGGCAAGCACCGTGGCATCCCGCAGCACTGAGTGACCTGGGCTCCTCA AGCCATCTCATTGGTGAAATGACAGTGCCAGTACCCTCTCAGCTGGCTCTTGGAGGCCTGTGCATGGGGT CTGCACAGAGGAGGCCCCCAAACTATGCATGGACGGACACGTGATGCCTAGCACTTCCCTTGGTTGTGTC TCTGCCAACCCCAGGCTCTCACCCAGCAAGGAAATGAAATCCACTTTTATGACACATCTCCCTCCCCCAG CCAGCTCCATTCACCTATATGCCAGGGTGGTCCCTTTCAATGTCTGTCCCCCATTGGATGAATAAACAAG CGAAGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5325123 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5325123 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7215 same embryo
	EMAGE:7217 same embryo
	EMAGE:7214 same embryo
	EMAGE:7218 same embryo
	EMAGE:7219 same embryo
	EurExpress:euxassay_004333 same experiment
	MGI:4826036 same experiment
	EMAGE:21612 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source