EMAGE mouse gene expression spatial database

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EMAGE:7218

Ctdsp2 CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2 ( MGI:1098748)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004324. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004324_01	euxassay_004324_02	euxassay_004324_03	euxassay_004324_04

euxassay_004324_05	euxassay_004324_06	euxassay_004324_07	euxassay_004324_08	euxassay_004324_09

euxassay_004324_10	euxassay_004324_11	euxassay_004324_12	euxassay_004324_13	euxassay_004324_14

euxassay_004324_15	euxassay_004324_16	euxassay_004324_17	euxassay_004324_18	euxassay_004324_19

euxassay_004324_20	euxassay_004324_21	euxassay_004324_22	euxassay_004324_23	euxassay_004324_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

EMAGE:7218

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7218_wholemount_strong.wlz

7218_wholemount_moderate.wlz

7218_wholemount_weak.wlz

7218_wholemount_possible.wlz

7218_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7218_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

two stars

Text Annotation:

Structure

Level

Pattern

Notes

limb

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 20 21 22 23 24

gland

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

alimentary system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

integumental system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

cardiovascular system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

urinary system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

reproductive system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

respiratory system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

skeleton

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tail

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5101
Entity Detected:	Ctdsp2, CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2 ( MGI:1098748)
Sequence:	sense strand is shown >T5101 CCACGCGTCCGCGCATGGTCTGCCCTCAATGAAGATGGCTCCTGGGAGTGCATTCCTTCCTGCCGGGTTA GGGTTGGGCCTGTTGAGGCAGCAGAAGCCTGGTAACATGGTAGAGCTGTCCATGGTGGGGCAGAGGTCTC AGTGTCTCGACCTGGGTATCTAGGCCTGCTTGCTTTTCTGTCAGTGCACAGGCAGAGCCGGTGCTGTGTC CTTGGCCTTGTGGGGTTCCTACAGCAATCCACTCTGTCTCCCAGGTATATGTGCTCAAGAGGCCTTACGT CGATGAGTTCCTGAGACGGATGGGGGAGCTCTTCGAATGTGTTCTCTTCACTGCCAGCTTGGCCAAGTAT GCTGACCCTGTGACTGATCTCCTGGACCGGTGCGGGGTGTTCCGGGCCCGCCTGTTCCGAGAGGCTTGTG TGTTCCACCAGGGCTGCTATGTCAAGGACCTCAGCCGCCTGGGAAGGGACCTGAGGAAAACTGTCATCCT GGACAACTCCCCTGCATCTTACATCTTCCACCCAGAAAATGCAGTGCCTGTGCAGTCCTGGTTTGATGAC ATGGCAGATACAGAGTTGCTGAACCTGATTCCAGTCTTCGAGGAGCTAAGTGGAACTGATGATGTCTACA CCAGCCTTGGGCAGCTGCGGGCCCCTTAGCCTTCCTGCTCCAAGAAACGGCCATCCCAACAGGGGACTCT GCTACACTGTGCCTTTGTGGTCAGCCTCACTGACAGGGTCTGCAAGTAGCCAGGGCGTGTGGGACAAAAG CAGGTCACACGCAGAGCAGGAAATCAGACCAAGACCCAGAGCTCTCTGCACCCCGTGCTCAGTTCCCAGG TGGTTGTGTGGGTGTGTGTGCGTGCATGTGCCCGTCCGGTAAACTATGGTCCTGGGTGCCCAGTTGTGCA GTAGGGGGAGAAGCTGAAAGATAAGGACTCAACCCAAGTCATTTGTCTTCTTTCCTGCTATGCTGTGAGC TACTGAGCTTTACAGCCATGAAAACTATTGTAGGCTCCCTCGCCAAACCATGGCTTTCCCTCAGCATCAA GACCTGTGCCAAGGAGAAGGGAGAGGCCGGAGGCTGCTTTGGATGCCCAGACACACATCTTTCAGAAAAC ACAGAAACCTAGCCAGCAGCTGCAGACATGTTTGGGAATATGAGAGCATGTTCGCAGACCCACCAGGTTC TGTGTCCTATGGGCTACACCTGGCTCCAGCAAGAATGCCTGCCATGGGCACTTCTCTGTACCCGGGCTCC AGTGCCAGGCCAGCCTCTCACTCCCAAGCCATAGTCCTACTCCCATGGGAAAGGAGGTCTTCTCTCTGCC CTGGAGGAGGACATATAGCTGATTTCTGTTCTTTTAACTCTTAAAAATCTCTTTCTAAACTTGGAGTGGT TTATATTCGCCCGAGCTCCAGTCCTGGGCCTGAGGTTTGTGTGGGAAGCGCTGGGGTACAAAGAAAATGC AAATGTTCAATGCAAGATGTCTGTCTCCTTGCGTGTAGATCCCAAAAGTGCTCTTCAGGGGTTCTGTGCT AGCTGATGCAAGGACATTAGCTCCAAAGGCTGGGGAGGGCTTGGCTCTCCCTCTGTGTCTTCCTGGTGTC CCGCCCTGGCCAGCTCTCGTCTGCTCTCGCCTTTATCCTTCTCGGCTGCTGCTCTTCCTCTCCAGAGCCT TGGGAGAAGGACTTTCCCCGGTTGTACTTAATTGATTGGTTGTCCCAAGTGTTGCTCTTACTTGATTTGC CAAATGCCTCCTGCTTCTCGTTATAGGACTGAGTGAGGTCCTCATAGCTCTGTGCTCTGGGCAGCTCTGA GCTCTCAGCCCTGTTGCTTTCAATGTCAGTCACAGAGTGGAAGTTTCCCCAAAGTGGAAAGCTACCATTG GGAACACAAGGGGCCCCTGAGGGGCCTCCACCTCCGCATCACTGCCTTAAGTGAGTCCCGCCCACTCCCT GCCTCCCTCCGAGGTGGGCGGGAGTTCCTACCCCATCTCTGCTTCCCCTGGAGGCCGGCCAGCGTTTGTG GGTTGGTTCAGGTGTGGTACCTCCCACTCAGGAGTCAATATTTTCAAATTTTTCTAGTTTCCACACACGA GGTCCTGCCTGGGGTTTGAGAAAGCCTGGTGGAGGAGTGTGGTGGTGTGAGTGGAGACTGGCTGGCATTC CTCCAGCACTCTGCAGACTTCTTCCTGGGCCCGAGGCTATGGAGCCTTGGCTTTCACTTCTGTGACAAAC ACATGCCATTTCCCCAGCCTCATTTGCTGGTGTCTGTAGGGTCCTCCCAAGGTGAGCTGAGGGCTGCAGG TGCTAGCCAGGAACATGGGAGCTTGACTGTGGCTTCAGGTCCAGACCCTGATCTCACTGGCATCCCAATA CTGCAGGAGGGGTCTTCTCACAAAGCTCTGAGGGCTGGTCTTGACTAAGCAATAAGAGCCTCAGAGTTGA TTGAATTTTAAGGTCCCTTTCTGGCTTCTTGGTTCTCCATGTGACCAAAGCCCAGTGTGGCTTCATGGGA GCCCTGTTACTTCTCCATGAAACCCCAGCTCTCTAGGCCTGACTGCCACCAGAGTCCTCTTACCACCTGC AGTCTGCCACAAAGATGCCATATCAACTCCTCTCTTCCCAAAACTAAACAGGATCTATCATGGTGGCACG GAGTCAGCTAGATGTTGTCTCTTGCTATCCCTGTGCTAGTTCCATGTTTTAGGAGCAAAGTGATACTGGG GAGGAGAGGAGGGCAAGTGAGAGGAGCCAGTGCCTCCTGAAGCCCTTGCTCCACTGCCACCAGCCCGCCT GCCCCAGTGCAGACTGGTCTTCAGGAGTCAGTAGACCACCGGACCAGGTGTCAGCATCCACTCCAAGCCA TGCTAGGAATCTGCCCCCTGCCGGGTTCAGCTCTTTAAGGTGCCTCTTCAGGGACACAGTGTGTGTCTGA TTGGGCTTCGCATCAAAAGCCTGACGTTCCTGTCCCTCTTATAGGGGGAGCTTTGGACACAGGACCAGTT TGATAAAGGGCCAGGTAAGGGTTTCCACTCTGCACATTGTAGAGGGGACACCGAAGGCCCATGGGTCCCT AATTAAAGAAGTTGAGTGAATTTGCCTTCGTTCAACCAGGGACCTATCTCACTCCCTCCCAAAGATTTTT GTATAATTGTATAACCCCCCTCCCCCTGTTAACAGTGGCTCAGAAAAGCTTTGTACTAAAAGCATGGGAC CCTCACAGCAACCATTCCTGGGAGATGGGTCCCTTATAGGTCCATGACCTGCTCTGTTCAGAGGCCTCTT GGCAGTGTGGCCTGTTTGTGCTGTCCTGCATCCAGTGTGTTGGCCTGGCTAAGTGAAAACGGTGAGCTGT TTCCCCTTTGGGCTACTTAACCACGGCTACTCCTTTTTTAAAAATGTCCTAGTTCTCCCCTACACACACA CACACACACACACACGGTTTGATGAATTTCCAAACCACGGCTACTCCTTTTTTAAAAACGGGTTTGAACT CCTGTTTAGCCAAGAGTGATCTTGAGCTGGTCTCCTGCCTCCACCCCCTGAGTGCTGGGGTTACAGGTGA TAGCTGGTCTCCTGCCTCCACCTCCTGAGTGCTGGGGTTACAGGTGATCGCTGGTCTCCTGCCTCCACCT CCTGAGTGCTGGGGTTACAGGTGATCGCTGGTCTCCTGCCTCCACCCCCTGAGTACTGGGGTTACAGGTG TGATCACCTTGCTGGCTTGTGAGGTGCTGGGGACAACCCAGGGCTTCGTGCATGTCAGGCACACACTCCC AACTAAGCTACATCCCCAGTCCCTCTTTCTAGAAAACAGATAAGTCCATGTTCAGGGGAGAAGAGGTCAC AGGTCAGGCCAGCTGCCCCATCCTTTAGTTCGGGGTTAGCTCCCAGAACTGCCCTGCACCATAAGCTGGT GATTCTCCATGCCTGTCCTTACCCCGGATCTGGCTATTCCCGTGATGCCATTTGGAGGGGAGAGGTCTGC TTCCCACTGTGACCAGGCTATGGGATCCTTGGCCACCTTGCTTCATGATTTGATACATTTGTTGTATTCA AAAACTTGAACTGTAGGATGCCATTAAGAGTCTGTTTATATTTTTGGAATATTTGTATTACAATTGTTAA TAAAGGCTGGTTTAAAAACCTGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5324816 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5324816 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7215 same embryo
	EMAGE:7217 same embryo
	EMAGE:7214 same embryo
	EMAGE:7219 same embryo
	EMAGE:7216 same embryo
	EurExpress:euxassay_004324 same experiment
	MGI:4824100 same experiment
	EMAGE:21612 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source