EMAGE mouse gene expression spatial database

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EMAGE:7238

Ccnb2 cyclin B2 ( MGI:88311)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004323. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004323_01	euxassay_004323_02	euxassay_004323_03	euxassay_004323_04

euxassay_004323_05	euxassay_004323_06	euxassay_004323_07	euxassay_004323_08	euxassay_004323_09

euxassay_004323_10	euxassay_004323_11	euxassay_004323_12	euxassay_004323_13	euxassay_004323_14

euxassay_004323_15	euxassay_004323_16	euxassay_004323_17	euxassay_004323_18	euxassay_004323_19

euxassay_004323_20	euxassay_004323_21	euxassay_004323_22	euxassay_004323_23	euxassay_004323_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7238_wholemount_strong.wlz

7238_wholemount_moderate.wlz

7238_wholemount_weak.wlz

7238_wholemount_possible.wlz

7238_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7238_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

weak

regional

weak expression: see section 04 05 06 07 08 21 22 23 24

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 09 10 11 15 16 17

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5890
Entity Detected:	Ccnb2, cyclin B2 ( MGI:88311)
Sequence:	sense strand is shown >T5890 GCCTCGTCCGCACTTGCCAGGGCGGCCCTCATGGCGCTGCTCCGACGCCCGACGGTGTCCAGTGATTTGA AGAATATTGACACAGAAGTTATTCCTAAAGCCAAGAGCCATGTGACTATCCGGCGGGCAGTTTTAGAAGA AATTGGAAATAAAGTTAGAAACAGAACCACTCAGGTGGCGAAGAAACCTCAGAACACCAAAGTACCAGCT CTGCCCACCAAAGTGACAAATGTCAACAAGCAGCCGAAACCCACAGCCTCTGTGAAACCAGTGCAGATGG AGGCGCTAGCTCCCAAGGATCGTCCTCCCGCCCCTGAGGATGTCTCCATGAAGGAAGAGAGCCTCTGCCA AGCTTTCTCTGATGCTCTGCTCTGCAAGATCGAGGACATAGATAACGAGGACAGGGAGAACCCTCAGCTC TGCAGTGACTACGTGAAGGACATCTACCAGTACCTCAGGCAGCTTGAGGTTTTACAGTCCATTAATCCAC ACTTCTTAGATGGAAGAGATATAAATGGACGTATGCGTGCCATCCTGGTGGACTGGCTGGTCCAAGTCCA TTCCAAGTTTCGGCTTCTGCAGGAAACTCTGTACATGTGCATTGCCATCATGGACCGGTTCCTGCAGGCT CAGCTGGTCTGCCGGAAGAAACTGCAGCTGGTCGGGATCACAGCTCTGCTCCTGGCTTCCAAATATGAGG AGATGTTTTCTCCAAATATCGAAGACTTTGTTTATATCACAGATAATGCTTACACCAGTTCCCAAATCCG AGAAATGGAGACTCTGATTTTGAAAGAGTTGAAATTTGAGTTGGGCCGACCTTTGCCACTTCACTTCTTA AGGCGAGCCTCAAAAGCCGGAGAGGTGGATGTTGAACAGCACACTTTAGCCAAGTACCTGATGGAGCTGA CGCTCGTCGACTATGACATGGTGCACTACCATCCTTCTCAGGTGGCAGCCGCTGCCTCTTGCCTGTCTCA GAAGGTGCTGGGCCAAGGAAAATGGAATTTGAAGCAGCAGTATTACACAGGCTACATGGAGAGTGAAGTC CTGGAAGTCATGCAGCACATGGCCAAGAACGTTGTGAAAGTCAATGACAACCGTACCAAGTTCATCGCTG TCAAGAACAAGTATGCCAGCAGCAGACTCCTGAAGATCAGCACGATCCCTCAGCTGAACTCCAAAATCAT CAAAGACCTGGCCTCCCCTCTGCTGGGCAGCCCCTAGGCACTGGACTGCCATCCTGCGCTTCTCAGATCC TGTATGTATTTTATTCTAGTTTACATCACAAACCTCTTCTCAGACTCATTTTCTAATTGTGTATTGATGA AAAATAAAGCTATTGATTTTCTTATACTATCCTGTGTATGTTTGTATACTCTACACTGAAGAAATGTAGA ATGGCCTACAATAACTCTGCTCTGTATTGAAGGAAAATAAAGCTATTGATCTACAAAAAAAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3156950 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3156950 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7237 same embryo
	EMAGE:7239 same embryo
	EMAGE:7241 same embryo
	EMAGE:7240 same embryo
	EurExpress:euxassay_004323 same experiment
	MGI:4823706 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source