EMAGE mouse gene expression spatial database

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EMAGE:7239

Wbp11 WW domain binding protein 11 ( MGI:1891823)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004348. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004348_02	euxassay_004348_03	euxassay_004348_04	euxassay_004348_05

euxassay_004348_06	euxassay_004348_07	euxassay_004348_08	euxassay_004348_09	euxassay_004348_10

euxassay_004348_11	euxassay_004348_12	euxassay_004348_13	euxassay_004348_14	euxassay_004348_15

euxassay_004348_16	euxassay_004348_17	euxassay_004348_18	euxassay_004348_19	euxassay_004348_20

euxassay_004348_21	euxassay_004348_22	euxassay_004348_23	euxassay_004348_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7239_wholemount_strong.wlz

7239_wholemount_moderate.wlz

7239_wholemount_weak.wlz

7239_wholemount_possible.wlz

7239_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7239_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5888
Entity Detected:	Wbp11, WW domain binding protein 11 ( MGI:1891823)
Sequence:	sense strand is shown >T5888 GGCGGTTCCCGGGTGGTTGACCGAGGGGCAGAGGTGAAGGCCCCTTCGCAGAGAGTCCTCCGGGACCGCC CGCTAGCTGCTCTCGCCGTCCCGGGAGCCTCGCAGTCGTTTTTTTTTTTTTTTAATGAAGAAGTAAATCA TATAAATTGTCACCATGGGACGAAGATCAACATCCTCCACCAAGAGTGGGAAGTTTATGAACCCCACAGA CCAAGCCCGAAAGGAAGCCCGAAAAAGAGAACTGAAAAAGAACAAAAAACAGCGCATGATGGTCCGGGCT GCAGTTTTGAAGATGAAAGACCCCAAACAAATTATCCGGGACATGGAGAAATTGGATGAAATGGAGTTTA ACCCAGTGCAGCAGCCACAGTTGAATGAGAAAGTACTGAAAGACAAGCGAAAAAAGCTGCGGGAAACCTT TGAACGCATTCTACGCCTTTATGAAAAAGAAAACCCAGATATTTACAAAGAACTGAGAAAGCTGGAAGTG GAATATGAACAGAAGAGAGCTCAGCTGAGCCAGTACTTTGATGCTGTTAAGAATGCTCAGCATGTGGAAG TAGAGAGCATCCCTTTGCCAGACATGCCGCATGCTCCTTCCAACATCTTGATCCAGGACATTCCTCTTCC TGGTGCACAGCCCCCCTCCATCCTGAAGAAAACCTCAGCGTATGGGCCTCCAGCTCGGGCCGTGTCTATC CTTCCTCTCCTGGGACATGGTGTTCCCCGCTTGCCCCCTGGCAGAAAACCGCCCGGCCCTCCACCTGGCC CCCCTCCTCCTCAGGTTCTGCAGATGTATGGCCGGAAAGTGGGCTTCGCTCTAGATCTTCCCCCGCGGAG GCGAGATGAGGACATGCTGTATAGTCCAGAGCTTGCTCAGCGGGGTCATGACGATGACATGTCCAGCACC AGTGAAGACGACGGCTATCCTGAGGACATGGATCAGGATAAGCATGATGACAGTACTGAGGACAGTGACA CTGACAGGTCAGATGCAGAGAGTGACGGGGATGAGTTCGGGCACCGCGAGGACAGTGAGCGCGACAACAC TGAGGAGAAGAAGTCAGGTCTAAGCGTCAGATTTGCTGATATGCCTGGGAAATCAAGGAAGAAGAAGAAA AACATGAAGGAGCTGACTCCTCTTCAAGCCATGATGCTGCGAATGGCAGGTCAGGAAATCCCTGAGGAAG GACGGGAAGTGGAGGAATTTTCAGAGGAGGAGGATGCCGACGACTCGGATGATTCTGAAGCCGAGAAACA GTCGCAGAAGCAGCATAAAGACGATGCCCACTCTGACAGCACGGCTGCAGCCTCTTCCCAGCAGCAGGCT CCTCCACAGTCTGCTCCTGCTTCTCAGATACAAGCCCCTCCCATGCCAGGACCACCACCTCTTGGACCAC CACCTGCCCCACCCTTACGGCCTCCTGGACCACCTACAGGCCTTCCTCCTGGGCCGCCTCCAGGGGCTCC TCCATTCCTGAGACCACCTGGAATGCCAGGAATCCGAGGGCCTTTACCACGACTTTTACCTCCAGGACCA CCACCAGGCCGACCTCCTGGCCCTCCCCCAGGCCCACCTCCAGGTCTGCCCCCTGGACCCCCTCCTCGGG GACCCCCACCAAGGCTACCTCCTCCTGCACCTCCAGGTATCCCTCCTCCTCGTCCTGGCATGATGCGCCC ACCCTTGGTTCCTCCTCTTGGACCTGCCCCGCCTGGCCTTTTCCCACCAGCTCCCTTGCCCAACCCAGGG GTGTTGAGTGCCCCACCCAGCCTCATTCAGCGACCGAAGGCGGATGATGCAAGTGCCGCCACCATCGAGA AGAAAGCCACAGCGACCATCAGTGCCAAGCCCCAGATCACCAATCCCAAGGCAGAGGTCACACGGTTTGT GCCCACTGCACTGAGGGTACGCCGAGAGAATAAGGGGGCTACTGCTGTTCCCCAAAGAAGGTCAGAGGAT GACTCTGCTGTGCCTGTTGCCAAAGCAGCCCCGAGATCTGGTCCTTCAGTTGCTGTCTCAGTACAAACTA AGGACGATGTTTATGAAGCCTTTATGAAAGAGATGGAAGGGCTGCTGTGACAGGGCCTCCTGCCAGGACA GGCTTCTGTTCAGAGTACTGGTTTGTGGAACAAGAGGCTCTTATTACAGTGAAGAGCAATGTCCACTGTC AGGTGTTTTCCAAGTCCAGTTCCAGGAGCCTCCTCATGTTTGCCTGCTGGGATCCCTGCACAAGGTGTTT CTTTACAATAGACCAGGATGTGAAGCACTGCTGCTAACCCCTGCTCCCTGTGACATCACCATCTCCTCCC TCTCTTCCTCTCTCCATTTTGGGAATTCTGTCAGCGAGGTCTTAGTTACTTGTTTTTGACTCATGTGCCG TGGGCACTAGAGTAGAGAATTCTGGAGGAAAAAAGTTTATTTCATCTTGACTTTTGTGTTTGAGTTATTT TTAATATTTTCCTGTAAATATTTTGTAATATTTTCCTTGTAGTGAAATGGATTACAGTATCATTTCCTAA TTCAAAACAGGATATGTGGGAAGAAAATATACAATTCTGGGATTAAAGTTACTTCCCACAAGCTAAACTC TTTTGAGTGTTTCATGGACAAATAAAATGTTCCATACCAAGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3156999 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3156999 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7238 same embryo
	EMAGE:7237 same embryo
	EMAGE:7241 same embryo
	EMAGE:7240 same embryo
	EurExpress:euxassay_004348 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source