EMAGE mouse gene expression spatial database

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EMAGE:7248

Ubap2l ubiquitin associated protein 2-like ( MGI:1921633)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004347. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004347_01	euxassay_004347_02	euxassay_004347_03	euxassay_004347_04

euxassay_004347_05	euxassay_004347_06	euxassay_004347_07	euxassay_004347_08	euxassay_004347_09

euxassay_004347_10	euxassay_004347_11	euxassay_004347_12	euxassay_004347_13	euxassay_004347_14

euxassay_004347_15	euxassay_004347_16	euxassay_004347_17	euxassay_004347_18	euxassay_004347_19

euxassay_004347_20	euxassay_004347_21	euxassay_004347_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7248_wholemount_strong.wlz

7248_wholemount_moderate.wlz

7248_wholemount_weak.wlz

7248_wholemount_possible.wlz

7248_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7248_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5073
Entity Detected:	Ubap2l, ubiquitin associated protein 2-like ( MGI:1921633)
Sequence:	sense strand is shown >T5073 CCACGCGTCCGGGTAAAGTTGGGAGCGCAGCGGCGTTGGCGGCGGCGGCGGCGGCAGCGGCAACGCGGCG GGGCCGGTATTCTTCCTTGTAAATACTGTTATTTGTATATACTGTAAATGATGACGTCGGTGGGCACTAA CCGAGCCCGGGGAAACTGGGAACAACCTCAAAACCAAAACCAGACACAGCACAAGCAGCGGCCACAGGCC ACTGCAGAGCAAATTCGACTTGCACAGATGATTTCGGACCATAATGATGCTGACTTTGAGGAGAAGGTGA AACAATTGATTGATATCACAGGCAAAAACCAGGATGAATGTGTGATTGCTTTGCATGACTGCAATGGAGA TGTCAACAGAGCCATCAATGTTCTCCTGGAAGGAAATCCAGACACGCATTCCTGGGAGATGGTTGGGAAG AAGAAAGGAGTCTCAGGACAAAAGGATGGTGGACAAACTGAATCTAATGAAGAAGGAAAAGAAAATCGAG ACCGGGACAGAGACTATAGTCGGCGACGTGGTGGGCCACCAAGACGGGGGAGAGGTGCCAGCCGTGGACG AGAGTTTCGGGGCCAGGAAAATGGATTGGATGGCACCAAGAGTGGAGGGCCTTCTGGAAGAGGCACAGAC AGAGGCAGAAGAGGCCGTGGCCGTGGCAGAGGTAGTTCTGGTAGGCGAGGAGGAAGGTTTTCTGCTCAAG GAATGGGAACCTTTAACCCAGCTGATTATGCAGAGCCAGCCAACACTGATGATAACTATGGCAATAGCAG TGGCAATACATGGAACAACACTGGCCACTTTGAACCAGATGATGGAACAAGTGCATGGAGGACTGCAACA GAAGAGTGGGGAACTGAAGACTGGAATGAAGATCTTTCTGAGACCAAGATCTTTACCGCCTCTAATGTGT CTTCAGTGCCTCTGCCTGCGGAGAATGTGACAATCACTGCTGGTCAGAGAATTGACCTTGCTGTTCTGTT GGGGAAGACACCATCTTCAATGGAGAATGATTCATCTAATCTGGATCCATCTCAGGCTCCTTCCCTGGCT CAGCCTCTGGTGTTTAGTAATTCGAAGCAGAATGCCATATCACAGCCTGCTTCAGGGAGTACATTTTCTC ACCATAGCATGGTGAGCATGTTGGGGAAAGGATTTGGTGATGTTGGGGAAGCTAAAAGTGGCAGTACAAC AGGCTCCCAGTTCTTGGAGCAATTCAAGACTGCGCAGGCCCTTGCTCAACTGGCAGCTCAACATTCTCAA TCTGGAAGCACTACCACCTCCTCTTGGGATATGGGCTCAACAACACAATCACCATCACTGGTGCAGTATG ATCTGAAGAGTGCAAATGATTCAACAGTGCACAGTCCTTTTACAAAGCGCCAGGCATTCACCCCATCTTC AACCATGATGGAGGTATTCCTTCAGGAGAAGCCACCTGCAGTGGCTACCTCGACAGCTGCACCACCTCCC CCGTCTTCTCCTCTGCCAAGCAAATCCACCTCGGCTCCACAGATGTCTCCGGGGTCTTCAGACAACCAGT CCTCCAGCCCTCAGCCGGCTCAGCAGAAACTGAAACAGCAAAAGAAGAAAACCTCCTTGACGTCTAAGAT TCCTGCTCTGGCTGTGGAGATGCCTGGCTCAGCAGATATCTCAGGGCTAAATCTACAGTTTGGGGCATTG CAGTTTGGGTCAGAGCCTGTCCTTTCTGATTATGAGTCCACCCCCACCACGAGCGCCTCCTCAAGCCAGG CTCCTAGTAGCCTGTATACCAGCACGGCCAGTGAGTCTTCATCTACAGTTTCATCTAATCAGAGTCAGGA GTCTGGTTATCAGAGTGGCCCGATTCAGTCGACAACTTATACCTCCCAAAATAATGCTCAGGGCCCTCTA TATGAACAGAGATCCACACAGACGCGGCGGTATCCCAGCTCCATCTCTTCATCACCCCAAAAGGACCTGA CTCAGGCTAAGAATGGTTTTAGCTCTGTGCAGGCCACGCAGTTACAGACCACGCAATCTGTTGAAGGTGC TACAGGCTCTGCAGTGAAATCTGAATCACCTTCCACTTCTAGTATACCCTCTCTCAATGAAACAGTACCT GCAGCTTCCTTACTGACGACAGCCAATCAGCATTCATCCTCCTTAAGTGGCTTGAGCCATACGGAGGAGA TTCCAAATACCACCACCACACAACATAGCAGTGCATTATCTACACAGCAGAATACCCTTTCTTCATCAAC ATCTTCTGGGCGTACTTCAACATCCACTCTTTTGCATACAAGTGTGGAGAGTGAGGCAAATCTACATTCT TCCTCCAGCACCTTCTCTACCACATCCAGCACTGTCTCTGCACCTCCCCCAGTGGTCAGTGTCTCCTCCA GTCTGAATAGTGGCAGTAGTCTGGGCCTTAGCCTGGGCAGCAACTCTACTGTCACAGCCTCAACTCGAAG CTCAGTTGCTACAACTTCAGGAAAAGCTCCTCCCAACCTTCCTCCTGGAGTCCCGCCATTGTTGCCTAAT CCATATATTATGGCTCCGGGGCTGTTACATGCCTATCCGCCACAAGTATATGGTTATGATGATTTACAGA TGCTCCAGACAAGATTTCCATTGGATTACTACAGTATCCCGTTTCCCACACCCACCACTCCACTTACTGG GAGGGATGGTAGCCTGGCCAGCAACCCTTACTCTGGCGACCTCACCAAATTCGGCCGCGGGGATGCCTCC TCCCCAGCCCCAGCCACAACCTTGGCCCAACCCCAACAGAACCAGACGCAGACTCACCACACCACGCAGC AGACATTCCTGAACCCGGCGCTGCCTCCTGGCTATAGTTACACCAGCCTGCCATACTACACAGGGGTCCC GGGCCTCCCCAGCACCTTCCAGTATGGGCCTGCTGTGTTCCCTGTGGCTCCTACCTCTTCCAAGCAGCAT GGTGTGAATGTCAGTGTGAATGCCTCGGCTACCCCTTTCCAACAGCCAAGTGGATATGGGTCTCATGGAT ACAACACTGGTGTGTCAGTCACCTCCAGTAACACGGGCGTGCCAGATATATCCGGTTCTGTGTACTCCAA AACCCAGTCCTTTGAGAAACAAGGGTTTCATTCCGGTACTCCTGCTGCCTCCTTCAACTTGCCTTCAGCC CTAGGAAGTGGAGGGCCCATCAATCCAGCCACAGCTGCTGCCTACCCACCTGCCCCCTTTATGCATATTC TGACCCCACATCAGCAGCCACACTCTCAGATCCTTCACCATCACCTGCAGCAGGATGGCCAGGATATCCT CACTCTCGTCGATGACCAGCTTGGTGAATAAGTATTACCGTACCAACTGGGGCCTCTTCTAGCAGGCTCG AAGGCTGTGGAATAAAGTGAAATCTTTGCCCTTTAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5321969 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5321969 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7253 same embryo
	EMAGE:7252 same embryo
	EMAGE:7251 same embryo
	EMAGE:7250 same embryo
	EMAGE:7249 same embryo
	EurExpress:euxassay_004347 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source