EMAGE mouse gene expression spatial database

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EMAGE:7249

Ripk4 receptor-interacting serine-threonine kinase 4 ( MGI:1919638)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004343. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004343_01	euxassay_004343_02	euxassay_004343_03	euxassay_004343_04

euxassay_004343_05	euxassay_004343_06	euxassay_004343_07	euxassay_004343_08	euxassay_004343_09

euxassay_004343_10	euxassay_004343_11	euxassay_004343_12	euxassay_004343_13	euxassay_004343_14

euxassay_004343_15	euxassay_004343_16	euxassay_004343_17	euxassay_004343_18	euxassay_004343_19

euxassay_004343_20	euxassay_004343_21	euxassay_004343_22	euxassay_004343_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7249_wholemount_strong.wlz

7249_wholemount_moderate.wlz

7249_wholemount_weak.wlz

7249_wholemount_possible.wlz

7249_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7249_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 10 11 12 13 14

adenohypophysis

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vibrissa

strong

regional

strong expression: see section 03 04 05 06 18 19 20 21

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 21 22 23

cornea epithelium

weak

regional

weak expression: see section 02 03 04 21 22 23

naris

moderate

regional

moderate expression: see section 10 13 14

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 14 15 16 17 18 weak expression: see section 07 08

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 10 11 13 14

esophagus

strong

regional

strong expression: see section 12 13

stomach

strong

regional

strong expression: see section 04 05 06 07 weak expression: see section 08 09 10 11

hindgut

moderate

regional

moderate expression: see section 14 15

rectum

moderate

regional

moderate expression: see section 15

midgut

moderate

regional

moderate expression: see section 13 14 15 weak expression: see section 12 16 17

midgut loop

moderate

regional

moderate expression: see section 13 14 15 weak expression: see section 10 11 12 16

oral epithelium

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

bladder

strong

regional

Expression in the fundus region.

kidney calyx

weak

regional

weak expression: see section 09 10 11 17 18 19 20

urethra of male

strong

regional

strong expression: see section 13 14 15

left lung

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14

right lung

moderate

regional

moderate expression: see section 15 16 17 18 19 20 21

trachea

strong

regional

strong expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5043
Entity Detected:	Ripk4, receptor-interacting serine-threonine kinase 4 ( MGI:1919638)
Sequence:	sense strand is shown >T5043 GCGCACGCGATGGAGGGCGAGGGCCGGGGCCGGTGGGCTCTGGGGCTGCTGCGCACCTTCGACGCCGGCG AATTCGCAGGCTGGGAGAAGGTGGGCTCGGGCGGCTTCGGGCAGGTGTACAAGGTGCGCCATGTGCACTG GAAGACGTGGCTCGCGATCAAGTGCTCGCCCAGTCTGCACGTCGACGACAGGGAACGAATGGAGCTCCTG GAGGAAGCTAAGAAGATGGAGATGGCCAAGTTCCGATACATTCTACCTGTGTACGGCATATGCCAGGAAC CTGTCGGCTTGGTCATGGAGTACATGGAGACAGGCTCCCTGGAGAAGCTGCTGGCCTCAGAGCCATTGCC TTGGGACCTGCGCTTTCGCATCGTGCACGAGACAGCCGTGGGCATGAACTTCCTGCATTGCATGTCTCCG CCACTGCTGCACCTAGACCTGAAGCCAGCGAACATCCTGCTGGATGCCCACTACCATGTCAAGATTTCTG ACTTTGGGCTGGCCAAGTGCAATGGCATGTCCCACTCTCATGACCTCAGCATGGATGGCCTGTTTGGTAC AATCGCTTACCTCCCTCCAGAGCGAATTCGTGAGAAGAGCCGCTTGTTTGACACCAAACATGATGTATAC AGCTTCGCCATTGTGATCTGGGGTGTGCTTACACAGAAGAAGCCATTTGCAGATGAAAAGAACATCCTAC ACATCATGATGAAAGTGGTAAAGGGCCACCGCCCAGAGCTGCCACCCATCTGCAGACCCCGGCCGCGTGC CTGTGCCAGCCTGATAGGGCTCATGCAACGGTGCTGGCATGCAGACCCACAGGTGCGGCCCACCTTCCAA GAAATTACCTCTGAAACAGAAGACCTTTGTGAGAAGCCTGATGAGGAGGTGAAAGACCTGGCTCATGAGC CAGGCGAGAAAAGCTCTCTAGAGTCCAAGAGTGAGGCCAGGCCCGAGTCCTCACGCCTCAAGCGCGCCTC TGCTCCCCCCTTCGATAACGACTGCAGTCTCTCCGAGTTGCTGTCACAGTTGGACTCTGGGATCTCCCAG ACTCTTGAAGGCCCCGAAGAGCTCAGCCGAAGTTCCTCTGAATGCAAGCTCCCATCGTCCAGCAGTGGCA AGAGGCTCTCGGGGGTGTCCTCAGTGGACTCAGCCTTTTCCTCCAGAGGATCGCTGTCACTGTCTTTTGA GCGGGAAGCTTCAACAGGCGACCTGGGCCCCACAGACATCCAGAAGAAGAAGCTAGTGGATGCCATCATA TCAGGGGACACCAGCAGGCTGATGAAGATCCTACAGCCCCAAGATGTGGACTTGGTTCTAGACAGCAGTG CCAGCCTGCTGCACCTGGCTGTGGAGGCCGGACAGGAGGAGTGTGTCAAGTGGCTGCTGCTTAACAATGC CAACCCCAACCTGACCAACAGGAAGGGCTCTACACCACTGCATATGGCTGTGGAGCGGAAGGGACGTGGA ATTGTGGAGCTACTGCTAGCCCGGAAGACCAGTGTCAATGCCAAGGATGAAGACCAGTGGACTGCCCTGC ACTTTGCAGCCCAGAATGGGGATGAGGCCAGCACAAGGCTGCTGCTAGAGAAGAATGCTTCTGTCAATGA GGTGGACTTTGAGGGCCGAACACCCATGCATGTAGCCTGCCAGCATGGACAGGAGAACATTGTGCGCACC CTGCTCCGCCGTGGTGTGGATGTGGGCCTGCAGGGAAAGGATGCCTGGTTGCCTCTGCACTATGCTGCCT GGCAGGGCCACCTTCCCATTGTTAAGCTGCTAGCCAAGCAGCCTGGGGTGAGTGTGAATGCCCAGACACT AGACGGGAGGACACCCCTGCACCTGGCTGCTCAGAGGGGGCATTACCGTGTGGCTCGCATTCTCATTGAC CTGTGCTCTGATGTTAACATCTGCAGCCTACAGGCACAGACACCTCTGCATGTTGCTGCAGAGACTGGAC ACACTAGTACTGCCAGGCTACTCTTGCATCGTGGTGCTGGCAAGGAGGCTTTGACCTCAGAGGGCTATAC TGCCTTGCACCTGGCAGCCCAGAATGGACACCTGGCTACTGTCAAGCTGCTCATAGAGGAGAAGGCTGAT GTGATGGCTCGGGGTCCCCTGAATCAGACAGCACTGCACCTGGCTGCTGCCCGTGGACACTCAGAGGTGG TAGAAGAGCTGGTCAGTGCTGACCTCATTGACCTGTCTGATGAGCAGGGCCTCAGCGCACTGCACCTGGC TGCTCAGGGCAGGCATTCACAGACTGTGGAGACACTGCTCAAACATGGAGCACACATCAACTTGCAGAGT CTCAAGTTCCAAGGAGGCCAGAGCTCTGCTGCCACGTTGCTCCGACGCAGCAAGACCTAGCTTGCCACCA CAAAACCAGGGCTCCGTGTAGGCTTCTGGACCATCCTTGTTTCCTCATGGGGACAGAATGGTCCTGGGAC ACTGCTCACCCTGTTGGTGGCCTGCCCATACACTGACCAAGCAGAGGCTAATGGACAAGGCAGGAGTAGC TGTCTTGGGGCACAGTAGCCAAAGTGTCTGATGTCAGATGGGACTAGGTTGGTGTCATGTCACTGTGGTA TTGATTGGCTGCTGATGCAGGCCTTTTATGACAAAGCCTATACAAGAATGTCTCCTCTGTCCATAGAGCA AGCCATTTCTGCTTGCTTGGAGCATGACATCTTCAGTAGAGATGTGGGAAGGGCAGTGTCCTTTGTCTTC TCATTGTGATGGGCAGAGTAGCTGTCTCTGAAGGCATAGTGGGTTCTTAATATATGAGTGACATGGTAGC TTTGCTTGAGACCTGTGAGGATCTGGCTGCTGGAGTCTAGAAAGGGAGTGATTATAAAGCCACAGGGTTG GTCCTAACACTGGACAGCCTTGCCAACATGAAACTGCTGTTTCATTTGGTATTTTGGTTTTGGTTTTTAG TTTTGATGTCTAGGTCACCATGCCTCGTTCCCCCGATTTCCCTGCTGAGTTCTCAGCTAAAATGTCAGAG CCATATATATAAAAGTTACCGGAAATTTTTTTGTAAATGGGTTTTATACTAAAAGTTGTTTAGTCAAACA GTTTGCTCTTTCAGGCTCTCTTGGTGAAGTGATGGTTTGGGCCAAGGGCTTTGCTGACTTGCCCTTTAGC AACTTCTGCTATGTTCCAGTTACAGTAGATGAATGTGGGCAGAGGTGGCCATTGGAGATTGTTGTACTCT GAGGAGTCAGATTCGATAGCCTTTTGTTGTACCTTCCCCATTTCTGTTCTGAACACTGTCACTGTAGAGA TGACCTGTGTGCAAACATGCTATAGCATGGTATGTGACACAGAATGATATTAATGTACTGTGTACTTTGA CATGAATCATGGACAGGATACTCTTTCATGACAGGAAGTAGTGGAGCTGGCTATGTTTTAATATGCCTCA ATTTGTCTTCACTGCTTCCCTCTCTTGTGTAAAACACGGGGACCATAGGAGATCTGTTTTATGTCAATAA AGGACTCCGCCTACTTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5294197 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5294197 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7253 same embryo
	EMAGE:7252 same embryo
	EMAGE:7251 same embryo
	EMAGE:7250 same embryo
	EMAGE:7248 same embryo
	EurExpress:euxassay_004343 same experiment
	MGI:4827748 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source