EMAGE mouse gene expression spatial database

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EMAGE:7250

Nup133 nucleoporin 133 ( MGI:2442620)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004337. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004337_01	euxassay_004337_02	euxassay_004337_03	euxassay_004337_04

euxassay_004337_05	euxassay_004337_06	euxassay_004337_07	euxassay_004337_08	euxassay_004337_09

euxassay_004337_10	euxassay_004337_11	euxassay_004337_12	euxassay_004337_13	euxassay_004337_14

euxassay_004337_15	euxassay_004337_16	euxassay_004337_17	euxassay_004337_18	euxassay_004337_19

euxassay_004337_20	euxassay_004337_21	euxassay_004337_22	euxassay_004337_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7250_wholemount_strong.wlz

7250_wholemount_moderate.wlz

7250_wholemount_weak.wlz

7250_wholemount_possible.wlz

7250_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7250_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5085
Entity Detected:	Nup133, nucleoporin 133 ( MGI:2442620)
Sequence:	sense strand is shown >T5085 CCACGCGTCCGCTTCCCGCCCCTCAGGTGTTCAAGCTCCGGGCGCGCGAGGTTCTCGCTATTAGCCCGCG AGTGCCGCTTCCTCCACGCTCTCTGCAAACATGTTTCCGAGCGTCTCCTCTCCGCGAACCCCAGGGCCGG GGACCCGACGCGGTCCGCTAGTCGGGATCGGGCCCACGTCCACGCCTAGAGCGAGCAGGAGGGGTCTGTC TCTGGGATCTGCGGTTAACTCGCCCGTGCTGTTCTCGCCCGCAGGCCGGCGCAGCTCGGTGAGCTCACGA GGAACGCCTACTCGGATATTCCCCCACCATTCCATATCAGAGTCTGTGAACTACGATGTGAGAGTGTTTG GATCATCTCTCCCTGTTAAGATCATGGAAGCCCTGACAATGGCTGAGGCTGACGAGCAGCTTTCTGTCCA TGTCGATGAAGGTGGGTGGGCCTGCCTGGTATGCACGGAGAAGCTCCTCATTTGGAAGATTGCTGTGTCG CCTGTCACTAAGTTGTCTGTCTGCAAAGAACTCCAGCTGCCCCCTAGCGATTTCCACGGGAGCGCTGATT TGGTGGCCCTGTCTTATGCTGCGACCTCAGGTGAAGTCCATTCTGTGCAGGCTGTTTCTGTCATGGTTGC TACCAAAGAGGGGTCCATCCGCTATTGGCCCAGCCTCGCCAGGGAAGACACCTACTCAGACACCTGTGTG GATTTGGGAGGCGAGAAGATGTGCAGGTTCCTGACAGCAGAGCAGGGAGGAAGTTTCATCCTGTCCTCCG TGGGGAGCCAGTTGGTTCGATTGATTCCTGAAAGCTCAGGAAAGATTCACCAGCATGTCCTGCCCCAGGG CCAAGGCATGCTCTCAGGGATTGGCCGGAGGGTTTCTTCCCTTTTCGGGATTCTGTCCCCTACTAGTGAC CTCATGCTGGCCAGTGTTCTGTGGGACAGAGGAGGATCAAGTTTCTACACCCTGAGGAGTTCAAACATCA GCAAATGGGAACTGGACGATTCTTCAGAGAAGCAGGTTCACAGCTGGGATGTGCACAGGACCCTGAAGGA GAGCATCACCGATGCTGTTTGGGGGTCTGAAAGTAACTATGAAGCTATTAAAGAAGGGGTCAACATTCAG TACTTGGACTTGAAACAAAACTGCGATGGGCTCCTGATTTTGGCAGCAGCGTGGCACCTGGGAGACAGCC CCTGTCTTGTCTATTACTCTGTGATCACGGTGGAAGACAATGGTAACCAGATGTCAGATGCAGTCACCGT AGAAGTGACTCAGTATAACCCCCCTTTCCAGTCTGAAGACCTGATCGCATGTCGATTGATGGTGCCAAAC TTTTCAAGCCAGATGACCTATCTTTACATGGAGAATGCTGTCTTTGTGTGCTCCACAGGCACTGGGAAGT TTTCTCTTCCTCAGGAAAAGATTGTCTTTGATACACAAGGGGATGGCATTTTGGGCGCTGGCTCCTGTGC TGGTGTTCCTATCCTTTTCTCTAGGAATAGTGGTCTGGTGTCTGTCATTCCAAGGGAAAATGTATCCCTG TTAGCAGAAGACCTGGAAGAGTCTTTGACCTCCTCAGTTGGTGGACGGGGCAGTGAGAGTATGGTTTTTG AGACCACCACAAAAAACGAAACTGTAGCCCATGAAGACAAGACAAAACTGCTTAAGGCTGCATTTCTACA GTACTGCAGGAAAGATTTGGGCCGCGCTCAGATAATGGCTGATGAGCTGTTTTCCTCTCACACGGATTTG GATTCGGACCCTGAGCTAGACAAGGCCGTGACTCAGATCAGTGTGGACCTGATTGATGACTACCCGGCCT CAGACCCACGCTGGGCAGAGTCTGTCCCCCAGGAGGCCCCTGGGCTGAGCAACACCTCACTGATCATTTT GCACCAGCTGGAGGACAAGATGAAAGCTCACTGCCTCTTGGTGGACTTCCTTCACCAAGTCGGCTTGTTT AGACGTTTGAGCTCCTATCCAATCAGAGGGACACCCATGTCCACCCGACTGCTACTCTGTGAACATGCAG AAAAGCTGTCAGCTGCCATCACACTAAAAAACCACCACTCTCGACTGCCAGACCTGGTGAACTCAGCTAT CCTGCTCGCCCTGAACAAGAGGGAGTGTGAGGTTCCCAACAGCCTGACTCCTGCCGATGTCTTCTTTCGA GAGGTGTCCCAGGTGGACACTATCTGTGAGTGCCTCCTGGAGCATGAGGAACAAGTCCTGCGTGAAGTTG CGTTGGTCTCTCAGGAGTGGGCAGAGGTGGCGATCGACGTCAATACTGTCCTCAAGGACATGCTACAGGC TGCCACTCACTACCGCCTAAACAAAAGCTCTATGTACAGTCAGGAAGAGGTACTAGGGAAGGAGCCAGAG TATGTGCCATGGACAGCCACAAGTGGTCCATCCGGTATCCGCACAGCAGTCATGCGCCAGCACGGGATCA TCCTGAAGATGGTATACCCTCAGGCTGACAGCAAACTTCGGAACGTTGTCATGGAGCAGCTTGTGGCACT AATTGACTGCTTCCTGGACAGCTACGTTTCCCAGCTGAAGTCGCTAGAGAAATCCAGTGACCAAGAACGA TACAGCAGTCTGGAAGTGGAGTACCTGCAGAAAAGATCCGAGCTGCTCTCGCCCCTTCTCACACTAGGTC AGTACCCATGGGCAGCTTCTCTGGCAGAGAAGTATTGTGACTTTGATATCCTGGTTCAGATGTGTGAACA GACAGACAACCAAGCCCGACTCCAGCGCTACATGACCCAGTTTGCTGACCAGAATTTTTCAGATTTTCTC TTTCGTTGGTACCTGGAAAAAGGAAAGCGGGGAAAATTGCTGTCTCAGCCCATTTCTCAGCATGGACAGT TGGCAAATTTTTTGCAAGCCCATGAGCATCTCAGCTGGTTACATGAAATTAATAGTCAGGAGTTAGAGAA GGCTCATACAACACTGCTAGGTTTGGCAAATATGGAAACTCGTTACTTTGCAAAGAAAAAAACCCTTCTT GGCTTGAGTAAACTGGCAGCTTTAGCTTCAGACATTTCAGAGGACAGGCTGCAAGAGAAGATTGAAGCCA TGGCTGAGCAAGAGCGCTTCCTGCTACACCAGGAGACCCTGCCAGAGCAGCTGCTGACCGAGAGACAGCT GAGTCTTAGTGCAATGCCTGTGTTGACCGCACCACAACTCATCAGTCTGTATATCTGTGACGAAAACAGA AGAGCCAATGAGTATGATTTCAAGAAAGCCTTGGACTTACTGGAGTACATTGATGAGGAAGAAGATGTGA GCATTGATGACCTGAAGCTGGAGATTCTGTGCAGAGCACTGCAGAGAGATGACTGGTCTGGTTCAGATGG CAAAGATGATCCAATCGAAGTGTCTAAAGACAGTGTGTTTGTAAAGATCTTGCAGAAACTTATAAAAGAC GGCATCCAGCTCAGCGAGTACTTGCCAGAGGTGACGGACCTACTGCGAGCAGAGCAGCTGGGAAGCCTCA AGTCCAACTCTTACTTTGAGTTTGTTCTGAAAGCAAATTATGAGTATTATGTCCAAGGACAGATGTGACA TTTGTAGAAATGGTTGTTTCTAAAATTTGTGTAAATATTTGCTTATTAAAAAAAAATATTGAGGCCAAAA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5323157 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5323157 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7253 same embryo
	EMAGE:7252 same embryo
	EMAGE:7251 same embryo
	EMAGE:7249 same embryo
	EMAGE:7248 same embryo
	EurExpress:euxassay_004337 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source