EMAGE mouse gene expression spatial database

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EMAGE:7251

Hmgcr 3-hydroxy-3-methylglutaryl-Coenzyme A reductase ( MGI:96159)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004330. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004330_01	euxassay_004330_02	euxassay_004330_03	euxassay_004330_04

euxassay_004330_05	euxassay_004330_06	euxassay_004330_07	euxassay_004330_08	euxassay_004330_09

euxassay_004330_10	euxassay_004330_11	euxassay_004330_12	euxassay_004330_13	euxassay_004330_14

euxassay_004330_15	euxassay_004330_16	euxassay_004330_17	euxassay_004330_18	euxassay_004330_19

euxassay_004330_20	euxassay_004330_21	euxassay_004330_22	euxassay_004330_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7251_wholemount_strong.wlz

7251_wholemount_moderate.wlz

7251_wholemount_weak.wlz

7251_wholemount_possible.wlz

7251_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7251_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

moderate

regional

moderate expression: see section 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 07 15 16

spinal cord

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 14

thoracic ganglion

moderate

regional

moderate expression: see section 12

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 12 14 15 16 17

nucleus pulposus

moderate

regional

moderate expression: see section 08 09 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5052
Entity Detected:	Hmgcr, 3-hydroxy-3-methylglutaryl-Coenzyme A reductase ( MGI:96159)
Sequence:	sense strand is shown >T5052 GGCAGCTGGGCCGGCTTGGCCTCCATTGAGATCCGGAGGATCCAAGGACTGTGAGGCTACAATGTTGTCA AGACTTTTCCGGATGCATGGCCTCTTCGTGGCCTCCCACCCCTGGGAAGTTATTGTGGGAACAGTGACAC TTACAATCTGTATGATGTCCATGAACATGTTCACCGGCAACAACAAGATCTGTGGCTGGAATTATGAGTG CCCCAAATTTGAAGAGGACGTGCTGAGCAGCGACATCATCATCCTGACGATAACGCGGTGCATCGCCATC CTGTACATTTACTTCCAGTTCCAGAATCTACGGCAGCTTGGGTCCAAGTACATTCTGGGTATTGCTGGCC TCTTCACAATTTTCTCAAGCTTTGTCTTCAGTACAGTCGTCATTCATTTCCTCGACAAAGAACTGACAGG CTTAAATGAAGCTTTGCCCTTTTTTCTGCTCTTGATTGACCTTTCTAGAGCGAGTGCATTAGCAAAGTTT GCCCTCAGTTCAAATTCACAGGATGAAGTAAGGGAAAATATAGCTCGTGGAATGGCAATCCTGGGCCCCA CATTCACTCTTGACGCTCTTGTGGAATGCCTTGTGATTGGAGTTGGCACCATGTCAGGCGTCCGCCAGCT GGAGATCATGTGCTGCTTCGGCTGCATGTCAGTGTTGGCCAACTACTTTGTGTTCATGACATTCTTCCCG GCCTGTGTGTCGCTGGTCCTAGAGCTTTCTCGTGAAAGCCGTGAGGGTCGTCCAATTTGGCAGCTCAGCC ATTTTGCCAGAGTTTTAGAAGAAGAAGAAAATAAACCAAACCCCGTAACCCAAAGGGTCAAGATAATTAT GTCTTTAGGCTTGGTCCTTGTTCACGCTCATAGTCGCTGGATAGCTGATCCTTCTCCTCAGAACAGCACA GCGGAGCAGGCTAAGGTTTCCTTGGGTCTGGACGAAGATGTGTCCAAGAGAATTGAACCAAGTGTTTCTC TCTGGCAGTTTTATCTCTCCAAGATGATCAGCATGGACATTGAGCAAGTGATTACCCTGAGTTTAGCCTT CCTTTTGGCTGTCAAGTATATTTTCTTTGAACAAGCAGAGACAGAATCGACACTGTCATTAAAAAATCCT ATCACGTCTCCCGTCGTGACCTCAAAGAAAGCTCAAGACAACTGTTGTAGACGTGAGCCTCTGCTTGTGA GAAGGAACCAGAAGCTTTCGTCAGTAGAGGAGGATCCAGGAGCGAACCAAGAGAGAAAAGTTGAGGTTAT AAAACCATTAGTGGTGGAAGCAGAGACTACAAGCAGAGCTACGTTTGTGCTTGGGGCTTCTGTAGCCAGC CCTCCATCCGCCCTGGGGACACAGGAGCCTGGAATTGAACTCCCCATCGAGCCACGACCTAATGAAGAAT GTCTGCAGATACTGGAGAATGCAGAGAAAGGTGCAAAGTTCCTTAGTGATGCAGAGATCATCCAGTTGGT CAATGCCAAGCATATCCCAGCTTACAAATTGGAAACTCTAATGGAAACTCATGAACGTGGTGTGTCTATT CGCCGGCAGCTCCTCTCCACAAAGCTTGCAGAGCCGTCCTCTCTACAGTACCTGCCTTACAGAGATTATA ATTATTCCTTGGTGATGGGAGCTTGCTGTGAGAATGTGATCGGATATATGCCCATCCCTGTTGGAGTGGC AGGGCCTCTGTGCCTGGATGGGAAGGAGTACCAGGTGCCGATGGCAACGACGGAAGGCTGTCTTGTGGCC AGCACTAACAGAGGCTGCAGAGCCATAAGCCTTGGTGGAGGTGCCAGCAGCCGGGTCCTTGCAGATGGGA TGACTCGAGGCCCAGTGGTGCGTCTTCCACGTGCTTGTGACTCTGCAGAAGTAAAGACCTGGCTTGAAAC ACCTGAAGGGTTTGCAGTGATAAAGGAGGCCTTTGATAGCACCAGCAGATTTGCTCGTCTACAGAAACTC CACGTGACGATGGCAGGACGCAACCTCTATATCCGTTTCCAGTCCAGGACGGGGGACGCCATGGGCATGA ACATGATCTCTAAGGGTACGGAGAAAGCACTGCTGAAGCTTCAGGAGTTCTTTCCTGACATGCAGATTCT GGCAGTCAGTGGGAACTATTGCACCGACAAGAAGCCTGCTGCCATAAACTGGATCGAAGGACGAGGAAAG ACTGTGGTTTGTGAAGCCGTCATTCCAGCCAAGGTGGTGAGAGAGGTGTTAAAGACAACTACGGAAGCTA TGGTTGACGTAAACATTAACAAGAATCTTGTGGGCTCGGCCATGGCTGGGAGCATAGGCGGCTACAACGC CCACGCAGCAAACATTGTCACTGCTATCTACATCGCATGTGGCCAGGATGCAGCACAGAATGTGGGGAGT TCAAACTGTATTACTTTAATGGAAGCCAGTGGTCCCACAAATGAAGACTTATATATCAGCTGCACCATGC CATCGATAGAGATAGGAACCGTGGGTGGTGGGACCAACCTTCTACCTCAGCAAGCCTGCCTGCAGATGCT AGGTGTTCAAGGAGCATGCAAAGACAATCCTGGAGAAAACGCACGGCAGCTTGCCCGAATTGTATGTGGC ACTGTGATGGCTGGTGAGCTGTCCTTGATGGCAGCCTTGGCAGCAGGACATCTTGTCAGAAGTCACATGG TTCACAACAGATCAAAGATAAATTTACAAGATCTTCAAGGAACGTGCACCAAGAAGGCAGCTTGAGGATC CTGACACTGAACTGAAGCGCGGGCATTGGGTTCTCAAGGACTAACATGCAATCTGTGAATTAAAAATCTC AATGCACTGTCTAGTGGAAGATGAATGGACATGATCAGTGACACCCCTGCTTGGTTTCTGGCGCTTTCAG AGACGTCTGAGGTTCTTTGCATAGAGACTCCTCAGATGTGGAAACTCTGGTTCTTTCCGTGCTGTGTTCT GGAAAGATCTCACGTGGATGGTACCGGTGCTCTGAGCACCACAGATGTGAGCTACAGTTCGTTTCTGAAA GCTACCACAAGCTGGAAACTGGTGATCAGTGTGGGGCTCACCTCTCCGTGGGTTAAAAATGGTTTTAAAT GACACTGTAGCTGACAGAACTTCTGATCTTTATTTATTCAGTCTGGGTTGTAGAACTTTGCAATCTAAGT TTATTTTTTGTAAACTAATAATTCATTTGGTGCTGGTCTATTGATTGGGGGCCTACTTCTTCATGGAAGA ATTACTTTTATTCTCAAACTACAGAATAATGTGCTAAGTAGTGCTAAATAGTTCTTGACGAAGAAAACAG TCACTGCATTTATCTCTGTGAGTCTTTGTTCAGAGAGGCCTTTAGTCTAGATTCTGCCAGCTGTGCCACA CTCTGCACTAAAGATATCAGAGCTCTTAGTGTTACTTAGAGGAGAGTACAAGCAAGTCGGACCTCTCAGA ACTTAGAGTGTGGGAACAGTTTTTTTTTTTTTTTTTTTAAAAACAAAAAACAAACGACCATTTCTCTAAG CCAAGTTTCTTTTAGAGACATTTTAACTTATTTAGCTGAACTCTAGATTTTTTGGTAAACTATCAAATCT GTATATGTTGTAATTAAGTGTCTAATGCTAGGAGTTTATTGGAAGTGTTTAAGAAATAAAAGAACTCAAC TTTTACACTGATAAAATACTCTAGCTTGGGCCAGAGAAGACAGTGCTCATTAGCACTGGTCCAGGAAACC CTGGCTTGCTTTCCAAGCCCAATGAAGGGAAAGTCAGCTTACAGAGCCAATGATGGAGCCACATGAATGG CCCTGGAGCTGTGTGCCTTGTTCCTGTGGCCAGGAGTTTGGTGACTGAATCATTTATGGGCTCCTTTAAT GGGCCCATAAAAGCTCTTAGCTTCCTCAGGGGGTCAGCAGAGTTGTTGAATCTTAATTTTTTTTTTTTTA ATGTACCAGTTTTGTATAAATAATAATAAAGAGCTCCTTATTTTGTATTCTAAAAAAAAAAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5320486 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5320486 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7253 same embryo
	EMAGE:7252 same embryo
	EMAGE:7250 same embryo
	EMAGE:7249 same embryo
	EMAGE:7248 same embryo
	EurExpress:euxassay_004330 same experiment
	MGI:4825393 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source