EMAGE mouse gene expression spatial database

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EMAGE:7321

Pvr poliovirus receptor ( MGI:107741)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004367. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004367_01	euxassay_004367_02	euxassay_004367_03	euxassay_004367_04

euxassay_004367_05	euxassay_004367_06	euxassay_004367_07	euxassay_004367_08	euxassay_004367_09

euxassay_004367_10	euxassay_004367_11	euxassay_004367_12	euxassay_004367_13	euxassay_004367_14

euxassay_004367_15	euxassay_004367_16	euxassay_004367_17	euxassay_004367_18	euxassay_004367_19

euxassay_004367_20	euxassay_004367_21	euxassay_004367_22	euxassay_004367_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7321_wholemount_strong.wlz

7321_wholemount_moderate.wlz

7321_wholemount_weak.wlz

7321_wholemount_possible.wlz

7321_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7321_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5301
Entity Detected:	Pvr, poliovirus receptor ( MGI:107741)
Sequence:	sense strand is shown >T5301 CCACGCGTCCGGAGATAAGGCGCTTGGCCGTTACTAACTGGACTACAAAGAGCTGGATCGGACCGGAACC ACATGGCTCAACTCGCCCGAGCCACCCGCTCCCCGCTGTCATGGCTGCTGCTGCTGTTCTGCTATGCACT CCGGAAAGCGGGTGGGGATATACGTGTGCTGGTGCCCTACAATTCGACAGGCGTCTTGGGAGGGTCGACC ACCTTGCACTGTAGTCTGACTTCTAATGAGAATGTGACTATCACTCAAATAACCTGGATGAAGAAGGATT CAGGTGGATCCCACGCTCTTGTGGCTGTCTTCCACCCCAAGAAGGGGCCCAACATCAAAGAGCCAGAGAG GGTGAAATTCTTGGCTGCCCAACAGGATCTGAGGAACGCATCTCTGGCCATCTCGAACTTAAGTGTAGAA GACGAAGGCATCTATGAATGTCAGATTGCCACATTCCCCAGAGGCAGTAGAAGCACCAATGCCTGGCTGA AGGTGCAAGCCCGACCTAAGAACACTGCAGAGGCCCTGGAGCCCTCTCCCACCTTGATACTGCAGGATGT GGCTAAATGCATCTCTGCCAATGGTCACCCTCCTGGACGAATCTCTTGGCCCTCGAATGTGAATGGAAGT CACCGTGAAATGAAGGAACCAGGGTCCCAGCCGGGCACCACCACAGTTACCAGCTACCTCTCCATGGTAC CTTCTCGCCAGGCAGACGGCAAGAACATCACCTGCACGGTGGAGCATGAAAGCTTACAGGAGCTGGACCA GCTGCTGGTGACCCTTTCCCAACCCTATCCACCTGAAAACGTGTCCATCTCTGGCTATGACGGCAACTGG TATGTTGGCCTCACTAACTTGACCCTGACCTGTGAAGCTCACAGCAAACCAGCGCCTGACATGGCTGGAT ATAACTGGAGCACGAACACGGGTGACTTTCCCAACTCTGTTAAGCGCCAGGGCAATATGCTTCTAATCTC CACCGTAGAGGATGGTCTCAATAACACGGTCATTGTGTGCGAAGTCACCAATGCCCTAGGGTCTGGGCAG GGCCAAGTGCACATCATTGTTAAAGAGAAACCTGAGAATATGCAGCAAAATACAAGATTACACCTAGGCT ACATCTTTCTTATCGTCTTTGTCCTCGCTGTAGTCATCATCATCGCAGCACTATACACTATACGAAGATG CAGGCATGGTCGTGCTCTGCAGTCCAATCCCTCAGAGAGGGAGAACGTCCAGTATTCATCTGTGAACGGC GACTGTAGACTGAACATGGAGCCAAACAGCACAAGGTGACGGTGCTGGGTAGACAGAACTAAGGAACTTG AAGGCATAGCAACTGGAACCCTACTCTCATAAATGAAGAAGCCTCCAGAGAGACTGGCTGCTCAGTGTGA TGAGCATAGCAAGTTTGGGGGGTCTCCCAGGATGCTGCCGAATTCCACGTTGTCAAAAGGACCCATGGAG GCCAGTGTGTTGGCTCACTCTTGACATCTCAGCAAGCTGGGGGGGGGGGGGGGAGCATAAAGCAAGGTTG AGTCTAGCTTGGGCTATAGAGCAAAGCCCTGTCCATACACAAACAAGCTAAGGGGCTTTGAGACGGTCAG AAACTGAAGTCTTGCTTTGGGTAAGGTAAATCCTCTACCGCATGTATGTGCTAGACTTGAAAGACTTCCA CACAGACCTCTTTATAAGTTGACTCCATTGGGGCTATCCCCTCCTCTCTGGACAAGGTCTCTGTATGTAG CCAAGGCTAGGCTCAAACTCACAGAGATATGTCTGCTTCTACCTCCCCAGTGCTAGAGTTGAAAGTATTT GTGCCACTGCACTTTTCTAGGTCTTCTTTTAATGAAGTAAAGTATATATTTATAAAAAGCTATTTAGTTA TATATATATATATTTTTGAGACTATTTCATAGAGCCCAAGCTAACCTCAAACTTACTATGTAGCCAAGAG TGATGGTAAACTAATTTATTTTAATTTATTTGTCTTCAATTTTAACCATCACCCAACCCCTGCTCCCTTC CATATCTTCTTTCAATCCATTTCATTGTCTTTTTCTTCCCAGACACTATTCTGACTTACGTCTCCATTAC AAACATTTTATTGAACTACATAAAAATGTGTGAACCACAAAAAAAAAATGTATTTGTCAAAATTGTAGTT GTCTTTCTGAGGCTGACCTGAGTTCTCTGATACCATTCTCTCCAGTTGTATCCAGTTTCCTGTAAACAAT GTGACTTTGTTTTTCTCAGTAGCTAAAACATCCCAATTATGTGAGTGTACACTTTCTTTACTCATTCCTC TGTGGGCCACCAGCTGGGTTGGTTCCATATCTGAGCTATTGTGCATGGAATTGTCTCTGTGGTGGGTTTA GTAAACTCCCAGGAATGCCTGTACATGTTTGTAGAGGCCAGAAGAAGGCACAAAATCTTGAGCCAGGCTT ACATGCACTTGTGAGTAGCCCCACATAGGTGCTAAGAACCCAGTTCAGGTCCTCTGCTGTGGGATGGTGG GCTGTGCACAGAAAGCCTGGTCCCGGTCTAGCAAAGGTCTGGAACTCCGGAGCCGGTGGGCTGTGATTTA CACCAGCATGGGATGGAAGGAGTTGGACCTCGCCTCCTGGGCACCTGGCTCCTGTCACATAGCTACAGCC TCCCACAGCCCCCCTATAGGGAGGTATGCAGCATCAATCACATAGTAGCTGCACTAAGCCCTCCCACATG CAAATAAGGTTTCCCCAAACTCTCAGTCCAAGCCAATGAAAAGTACCTGCTGTCAAACCCTAAATCATCC CCAAAACTCTGTAAGTCCTATCAGGGAATAAAATGTGTGTGAAAACTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5363187 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5363187 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7324 same embryo
	EMAGE:7320 same embryo
	EMAGE:7322 same embryo
	EMAGE:7325 same embryo
	EMAGE:7323 same embryo
	EurExpress:euxassay_004367 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source