EMAGE mouse gene expression spatial database

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EMAGE:7325

Rfwd3 ring finger and WD repeat domain 3 ( MGI:2384584)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004368_01	euxassay_004368_02	euxassay_004368_03	euxassay_004368_04	euxassay_004368_05

euxassay_004368_06	euxassay_004368_07	euxassay_004368_08	euxassay_004368_09	euxassay_004368_10

euxassay_004368_11	euxassay_004368_12	euxassay_004368_13	euxassay_004368_14	euxassay_004368_15

euxassay_004368_16	euxassay_004368_17	euxassay_004368_18	euxassay_004368_19	euxassay_004368_20

euxassay_004368_21	euxassay_004368_22	euxassay_004368_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7325_wholemount_strong.wlz

7325_wholemount_moderate.wlz

7325_wholemount_weak.wlz

7325_wholemount_possible.wlz

7325_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7325_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5146
Entity Detected:	Rfwd3, ring finger and WD repeat domain 3 ( MGI:2384584)
Sequence:	sense strand is shown >T5146 CCACGCGTCCGGCGGAGCGGGTGCGCAGTAGGGGATGCGCGGTAACGATGGCCGAAGTCGCGGTCGGCCA GGCGTCCGACTTGGTCCCCTCAGAGATGGATCATGAAGTGATATACAGTCATCTTCAGGGACCATTAGAG GGTACTATAGAACCAGCTACTCCAACTGAAGTCGTCAGCAATGGGGCACCTCTCCAGCCTGCCCCTGCTG AACTGGCCAATAGCCAAGGTGGAGCACATGTTCAGCCTGCCCCTGCTGAAGTGGTCAGCAGCCAAGATGG ACTGCCCACTCTACAGCCTCTGCCACCAGCATCCATTGATTTGACGGAGGAAGTACAGCCCTCAGAAGAA AATATGGAGGTTGTCAATCCTGGAACTTCAGAGGAGCCTAGTCAGGGATCTGGTGCTAACCCAACCGCCG GAGCTGCTAGATCCGTTTCAATGAACAACTTCATCAGCGGGCTGCAGAGGCTTCATAACATGCTGGAATT GCTGAGACCTCCACCTGCAGACCACAGTGTGGGGCCAATAAGAACAAGGAGGAGGATGGCACCCATTTTG AGGGCCAGAGCTGGAGAGTCTCAGAGGCAAGACAATGGCAGGTATGTGCCACATACACCACTATATGCCT ACTTTCAGGTAAGCAGGTCTCAGCCTTACCCACTACCCGCTGCTCATGATTCAGAGACCAGGAATCCACC GTCAGGCACTGACTCTGACAGCGATGGCTCTGCAGAGGATGAAGAGGTTGTTGTCCAGGCAGAAGAACCT GAAGCCAACATTCCAGAACAAGGAGTTATCGCAACAGACCAAGAAGCTACATCTGTCACAGGAGATGATG CAGTCCCTAAAGAGTCTCCCCAGAAGCCCAACATGCTATCTGCTATGGAAGATGAGGAAGGTGAAACTTG CACAATATGTTTGGAACAATGGACCAATGCTGGGGACCACCGGATCTCAGCGCTACGCTGTGGGCACCTC TTTGGATTTAGATGCATTTCTAAATGGCTCAAAGGACAAACACGAAAATGTCCTCAGTGCAACAAGAAAG CCAAGCACAGTGACATCGTTGTCATTTATGCCCGATCCCTGCGAGCTTTGGACACTAGTGAACAGGAGCG CATGAAAAGTGACTTACTGAATGAGCAAATGCTCAGGAAGCAAGCTGAGTTAGAGTCTGCACAGTGCCGG CTCCAGCTTCAGGTCCTTATTGATAAGTGCACTAAACTCAATAGCCGTGTCCAGGACTTGGAAAAGTTTG TTGTGCGGCATCAGAACTCAGCTGCGCAGCAGTGCAGTCGCTCGAAAGCACTTTGTGGGAAATGCCTGTC CTCCAGCCAGAGCCCAGGCAAATACAGCTCAGAAAAGACCTTCACCATATCTCAGACGGGAAAATGCCGA ATCTTGGCCTATTGTGATGGTCTGAGCTGCCTGGTGGCCTCCCAGCCCTCCCCTCAGGCCTCCTTTCTTC CAGGCTTTGGTGTTAAGATGTTGAGTACTGCCAACATGAAGAGCAGTCAGTATATTCCTATGCACAACAA GCAGATTCGTGGATTATCTTTCAGCAGTCAGTCCAAAGGCTTGCTCCTGTCTGCCTCCCTTGACTGCACT ATTAAGCTGACCAGCCTAGAAACAAATACTGTGGTGCAGACTTACAACACTGGGCGTCCTGTCTGGAGCT GCTGCTGGTGTCTTGACGAAAACAATTACGTCTATGCTGGACTAGCCAGTGGTTCAATTCTGATCTATGA CTTACGGAACACAAACACCTATATTCAGGAATTAGTTCCTCAGAAAGCCAGATGTCCTCTGGTATCCCTG TCATATTTACCCAAAGCTGCAGTGGCTGCATTTCCTTATGGTGGGGTGCTGGCTGGAACATTGGAGAATG CCTCCTTCTGGGAGCTAAAATCAGACTTTTCTCATAAGCCTCATGTGCTGTCCTTGGAGCCAGGGGGCTT TGTAGACTTTCAGACAGAGAGCAGCACCCGACACTGTCTTGTGACTTACAGACCTGATAAAAGCCATAAC ACTGTCCGAACTGTGCTATTGGAGATGTCTTACAAACTGGACGATGCTGGAGAGCCGGTCTGTTCCTGTC TTCCTGTACAAACATTCCTTGGGGGACCCACTTGCAAACTGTTGACCAAAAGTGCCATCTTCCAAAGCCC AGAGAATAATGGCAATGTCCTAGTATGTACTGGGGATGAAGCGTCACAGTCTACACTGTTGTGGAATGCT GGCAGTGGCTTGTTGCTGCAGAACCTGAAGGCTGGTGAACCTGTCCTGGACATCTGCCCATTTGAAACGA ATCAGAACAGCTATCTGGCCACCTTGACAGAGAAGACTGTCCATATGTACAAGTGGGAGTGAGCATGGTC TTGAGACTTGCAGACTCACCGCTGCTAGTTAACGTTGCATTGATTTAGCACCTAGTACCCCTGAGCAGAG ATATCTGTTTACATGCTGGGACTGGAGCGTCTGGATCATGGTTCATTGGATTAGTGTGGTCATGTATCAC TGAGACACCAGGATTGTGTGTGCTGTTGGCATCAAATGAGTACCCTTGGGAATCTTGCCTGGAAATTTTT TTACTTAAATTCTATACAGTCCTCTCAACTTGCTATTTAGAATATGAGTATGCACCAAGCATTTTGGGGT TGTGATAACCAACCAACCAACCTAAATTGTCTTGTCAGTTAGGCTAATTGGCTTAAAAGTGGTACATGAA AGGTCTGCCTTGCTTCTTGGATGCAAAAGATACAGGAGAGAGTCTTCCACTAAGCATAAAGGGCACCATT AATATCTTGTGTGGAATTTGACCTATTTCCAAGTCTGAATGCTCATAACCTTTCAGTGACTTGATTTCTC CCATACCCCTGCACTGCTGTCTGCCCCCAGTGTCTGGTACTTCTGTGAGATATGCTTTGTGGCAACAAGT TACATCCCAGGTATCCATGCCTACTAGAATGTTGATAAGTCCAATGGTGGAATTGGCTGACTAGCTAGCC ATATGTAAAGGCATAGCTTTAAGCTTTGTGCAGTAAGTTCATTGCATTGTGAGAGGTGAGGAAAGGGAAG AGGTGTTTGGGCAATTGTTAAAGACTTGAAATTTTCTAGTTAGCTTGAGATACTCAGGAGCCTGGCTTTT ACTCGAACTCACCCCCCAAAGTCAATGCTGAAGGATAGATAATTCCCAAGTAAAAACTTCCCCTCCTGTT CTACTTTTTTCTGAAAATGAAGTGGTTATTCTCAGGAAGTTAATAGTGGCTAGAGATCTGTAGGTGACAG AAAGGAAGACACTCAGAAACTTCCATTCTTGTGGTCCCAAATTGTCCAGCATTTAGAGGTATGCTCAGGT TATATTTGTCTGCTTTGCCTTAGGCTACTCCACTAAGCCACTGCCCCTAAGACACCTTGTCTCAAGGCAT AGGCAACATGTCTGATTCTCTCAGATGTTCTTTGATGAAGCACTATCTTTTTTAAATAAAAGTTCTTTCA TGCCTATCAAACTCAGCTATTTTGGGGACCTGTACTGCCAGCAAGGGATTATTAAGGGAAAATTGTTAAT TTTCCCAGAATTCCAAGTTGTGGCTTTATCTGCTTAATGAAACCTATTTGAAGATGTGTAATTTGTTTTG TTTTTAAAACTTAAAGTAAGCTTTAAAAATGTCAGCTTCCAGACAGTTGAGGTAATACATGCCTTTGGTC CCAGCACGCTGAAGGCAGAAACTGGTAGATCCCTATGAGTTCAAGGCCACCCTCACTACAAAGTAGGTTT TGGACAGCCAAAAGCTATAACACGGAGAAAAAGGGGGGGGGGGGGGCAAAAGAAAAATGTTAGCTTCCAC AGCGTAGAGGAAAGTCGCCTTCTCTCCCTTTCTTCCCCCAGGTGCTTTTGATAGAGTGATGCTTAAGAGT AGCTGCCTGGGCCTGGAGCTTCGATTTCAGGATAGGATTCAAATTAAATAGAATTCAACTAACTAAAGCA GATGGCCACAATAAAAGCTGTGCGTGTCAGGTTAGTCTGGGGCAGGGTTAGGATATGACTTGCCTTGAAG GACATTTAAGGCTGACTGGGGATTTGCTACCACAGAAGCTAAGCTAAATACCAAGCCTTGCCGTGGCAAT TTGCATTTCCACAATAAATTTGGATTGGGGTGGGGGAGTGCCAGGACCTCAACCTGGTTAAGGTTTTTGC TCTACCACTGAGCTGTATTAGAAGCCCAAATCTGTGATTTGGAAGCAGATTCACAGTATGGACCGATTGA CCAAAGACAACCTTATTGCAAGAAACTTCATCTCTAAACCTGGGCTCTAGTCTTAGCACCAGCAAGATCT GTTTTGTTTTAAAATCTTGTCATAGGGCATTGGGATTTCTGAATATCTGCTGTCCTGTGTGTGTTTCAAT GTTTTAATGTTAACAAAATAAAGAATTTGTAAGGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5344528 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5344528 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7324 same embryo
	EMAGE:7320 same embryo
	EMAGE:7322 same embryo
	EMAGE:7321 same embryo
	EMAGE:7323 same embryo
	EurExpress:euxassay_004368 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source