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EMAGE:7333

Atpaf2 ATP synthase mitochondrial F1 complex assembly factor 2 ( MGI:2180561)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7333 EMAGE:7333 EMAGE:7333 EMAGE:7333 EMAGE:7333
"Pseudo-wholemount" of euxassay_004454. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004454_01 euxassay_004454_02 euxassay_004454_03 euxassay_004454_04
EMAGE:7333 EMAGE:7333 EMAGE:7333 EMAGE:7333 EMAGE:7333
euxassay_004454_05 euxassay_004454_07 euxassay_004454_08 euxassay_004454_09 euxassay_004454_10
EMAGE:7333 EMAGE:7333 EMAGE:7333 EMAGE:7333 EMAGE:7333
euxassay_004454_11 euxassay_004454_12 euxassay_004454_13 euxassay_004454_14 euxassay_004454_15
EMAGE:7333 EMAGE:7333 EMAGE:7333 EMAGE:7333 EMAGE:7333
euxassay_004454_16 euxassay_004454_17 euxassay_004454_18 euxassay_004454_19 euxassay_004454_20
EMAGE:7333 EMAGE:7333 EMAGE:7333 EMAGE:7333
euxassay_004454_21 euxassay_004454_22 euxassay_004454_23 euxassay_004454_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7333Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7333_wholemount_strong.wlz
7333_wholemount_moderate.wlz
7333_wholemount_weak.wlz
7333_wholemount_possible.wlz
7333_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7333_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6444
Entity Detected:Atpaf2, ATP synthase mitochondrial F1 complex assembly factor 2 ( MGI:2180561)
Sequence:sense strand is shown

>T6444
CTGAGGGCTATGAACCCTTGGTGAGAGGTTTCCGTACAGAACCAGCGAGGGGTGTGGGGTTCTCTTCGCT
CTTTTCCTGTACTCTGTTTCCACGCATGTGTCCACCCAGGTCCTTGGGCAGGATGTGGCGAATCTACCCC
CGGCTGAGGGACCGGTGGCGCGGTCTCTTGGACCGGCGTCTCAGTGACCCCACCGTCTCAGTATGGCCGG
GGCCAGCTCCGCAGCCTCCGGCCCGGGCCTATGTCCCGCCAACAGAAAGGAAGAGGTTTTATCAGAATGT
CAGCATCTCACAGGGTGAAGGTGGCTTTGAGATAAACCTGGACCACAGAAAGCTGAAAACTCCCCAAGCC
AAGCTTTTTACTGTCCCCAGCGAAGCCCTGGCCATCGCAGTGGCCACTGAATGGGACTCTCAGCAGGATA
CCATCAAGTTCTACACCATGCACCTGACCACATTGTGCAACACATCCTTGGACAACCCAACCCAGCGAAG
TAAAGACCAGCTGATCCGGGCAGCTGTGAAGTTTCTAGACACTGACACCATCTGCTACAGGGTGGAAGAG
CCAGAGACATTAGTGGAACTTCAGAAGAACGAATGGGATCCCGTCATAGAGTGGGCTGAGAAAAGGTACG
GTATGGAGATCGGCTCCTCCACCAGCATAATGGGACCCAGCATCCCTACCCAGACCCGAGAGGTGCTTAC
CAGCCACTTGTCCTCTTACAACATGTGGGCTCTGCAAGGGATTGAGTTTGTAGTGGCCCAGCTCAAATCC
ATGTTGCTGACTTTGGGCCTGATTGACTTGCGCCTGACTGTGGAACAAGCTGTGCTTCTGTCACGCCTGG
AGGAAGAGTACCAGATCCAGAAGTGGGGCAACATTGAGTGGGCCCACGACTATGAGCTCCAGGAGCTGCG
GGCTCGCACTGCTGCTGGCACCCTTTTTGTCCACCTCTGCTCAGAAAGCTCTACAGTCAAGCACAAGCTC
CTTCAAGAGTGAGGCCCGGGGTGCACACGCTGTCTGGAAGGAATTGCACAGACACAGGCTCCCTGATGTG
GGCCTCCTTGGCTGTTGGACCTCTGGATCCTGTGATACTCTGTTGGCCGGTACCTACTGGGCTCCCTCAG
CCCTATACCTGTGGGGCTCCCCAGCCTGTACCTGGGTCTTCCCTGACCTGTGTGGTTTGACCTTATAAAG
AAGCATCCTGAGAAGCCCTGAAGACACAGTGTGCCTGACTTGAGGTCAGATAGCTTCCGATGCAGGCGCC
CTTGGTCTGACCAGAAGTGAGGGAGGAAGACAAGTGGTATAGGGACTTTCTCTTGACCCTGATTTTATTA
AATATTTTTTCATTCTGGAAATATATCAAGCCTCATTTTTATTTGAGCCTTGGCATTTCTCCATTCTTTT
TGTTTTATCTGAGCTCTGTCACCATTATTACATCTGCTACTAATGGCTAGGAACCTCATGGGGAGGGCAG
GAAAGCACATCGCCACATGCAAGCCTTTTCCTGAAGGCCTTCCACACCCCACACCCCATACTCCCTGGCT
TTTCATCAAATGATCTTTTAAAAGTGAATCTTTGGAGGGCTGGAGAGATGGCTCAGCGGTTACGAGAGCA
CTGACTGCTCTTCTCAAGGTCGTGAGTTCAATTCCCAGCAGCCACATGGTGGCTCACAACCATCTGTAAC
AAGATCTGGGGCCCTCTTCTGGTGTGTCTGAAGACAGCTACAGTGTACTTACATATAATAAATAAATAAA
TCTTTTAAAAAAAAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3585582 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3585582 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7335 same embryo
 EMAGE:7332 same embryo
 EMAGE:7334 same embryo
 EMAGE:7337 same embryo
 EMAGE:7336 same embryo
 EurExpress:euxassay_004454 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS