EMAGE mouse gene expression spatial database

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EMAGE:7335

Lypd3 Ly6/Plaur domain containing 3 ( MGI:1919684)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004451. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004451_01	euxassay_004451_02	euxassay_004451_03	euxassay_004451_04

euxassay_004451_05	euxassay_004451_06	euxassay_004451_07	euxassay_004451_08	euxassay_004451_09

euxassay_004451_10	euxassay_004451_11	euxassay_004451_12	euxassay_004451_13	euxassay_004451_14

euxassay_004451_15	euxassay_004451_16	euxassay_004451_17	euxassay_004451_18	euxassay_004451_19

euxassay_004451_20	euxassay_004451_21	euxassay_004451_22	euxassay_004451_23	euxassay_004451_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7335_wholemount_strong.wlz

7335_wholemount_moderate.wlz

7335_wholemount_weak.wlz

7335_wholemount_possible.wlz

7335_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7335_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

physiological umbilical hernia epidermis

moderate

regional

moderate expression: see section 16 17 18 19

epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

moderate

regional

moderate expression: see section 04 05 06 07 08 19 20 21 22 23

naris

moderate

regional

moderate expression: see section 12 13 15 16

genital tubercle of male

moderate

regional

moderate expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6445
Entity Detected:	Lypd3, Ly6/Plaur domain containing 3 ( MGI:1919684)
Sequence:	sense strand is shown >T6445 GGAGAGAGCAGGACACAGCTATGGATGCCGCCAGGAGAGGAGATACACAGCCAGTGATGTGGACCACCGG ATGGCTGTTGCTGCTGCCGCTTCTGCTGTGTGAAGGAGCGCAAGCCCTGGAGTGCTACAGCTGCGTGCAG AAGGCGGACGATGGATGCTCTCCGCACAGGATGAAGACAGTCAAATGTGGTCCCGGGGTGGACGTCTGTA CCGAGGCCGTGGGAGCGGTAGAGACCATCCACGGGCAATTCTCTGTGGCGGTGCGGGGCTGCGGTTCCGG AATCCCGGGCAAGAACGACCGCGGACTGGACCTTCACGGGCTCCTGGCCTTCTTTCAGCTACAGCAGTGC TCCGAGGACCGATGCAACGCCAAACTCAACCTCACTTTGCGAGGCCTCAACCCTGCAGGCAATGAGAGTG CATATGAGCCTAACGGTGCAGAGTGCTACAGCTGTGTGGGTCTGAGCCGCGAGAAGTGCCAGGGCTCCAT GCCGCCGGTCGTGAACTGCTACAACGCCAGTGGCCGTGTCTACAAGGGCTGCTTCGATGGTAACGTCACC CTGACGGCAGCCAACGTGACCGTGTCCTTACCTGTCCGAGGCTGCGTCCAGGACGAGACCTGCACCCGGG ATGGGGTGACGGGTCCAGGATTCACACTCAGCGGCTCTTGCTGTCAGGGCCCCCGCTGTAACGCCGACCT TCGCAACAAGACCTATTTCTCCCCTCGAATCCCACCCCTAGTCCTGCTGCCCCCTCCAACCACCGCAGCC CCATCCACTCGGGCCCAGAACTCCTCCAGCACGACCTCTACAGCAGCCCCAACCACGACCACCTCCATCA TCAAGCCCACCACAGCCCAAGCCAGCCACACTTCTCCCCATGAAATGGATCTCGAAGTCATACAGGAAGA GGGGGCGTCGTTGAGTGGAGGTGCTGCGGGCCATGGAGGTACTGCGGGCCATGGAGGTGCTGCGGGCCAC CAAGACCGCAGCAATATGGAGAAGTATCCAGGAAAGGGTGGGGCCCAGATCCCAGCTAAAGGAGGCTCTG GCACTCTAGGGTCCTGGTTGTCTGCAGTTCTGTTGACTGTGGTTGCTGGCGCGATGCTGTGAATGTCTCA TCTCGAAAAGTCCATCTCACTTTGTTTCCCTGGCCCCGTGGTACCAACTCTTTCCATTTCTCACTTGACT GGACTGGCTCCGCCCCCATCCTTCAGCATTCTCAGTTCCGACTGCACTGGTTTGCAGCTTCGGAAAACAG TCCTCTGTTGTAAATATTCCGCTCGGGTGGCCCTACTTTTTTGATGCGGCCACAGCATTCCCCCTGATGG TGACCAGGACAGAGGGAAGAGACGTCTACTGGCTGAGAGAGGCCCAGAGAGTCCACGGCAAGCCTCCTCT TCCCGTTTTCCTGACCAGGCTGGAAGATGACCAGGCAGGTAGACAATGGATCCATCCTCCGAGCACTGTG CTTGCCTGGCACATTGTGCGGAAATCTGGTCGCCTGTCTTCCTTAGGAGACTGTGAACAACTCTACAACA GGGTCTTGTCTCTGGCCTCTCTATGTGTTCTGTCTGGCACAGGAAGGTGTCAATAAAGATTTAGTTACTT TGTATAGTGAGTTAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3585545 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3585545 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7333 same embryo
	EMAGE:7332 same embryo
	EMAGE:7334 same embryo
	EMAGE:7337 same embryo
	EMAGE:7336 same embryo
	EurExpress:euxassay_004451 same experiment
	MGI:4826038 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source