EMAGE mouse gene expression spatial database

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EMAGE:7336

Tsnax translin-associated factor X ( MGI:1855672)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004475. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004475_01	euxassay_004475_02	euxassay_004475_03	euxassay_004475_04

euxassay_004475_05	euxassay_004475_06	euxassay_004475_07	euxassay_004475_08	euxassay_004475_09

euxassay_004475_10	euxassay_004475_11	euxassay_004475_12	euxassay_004475_13	euxassay_004475_14

euxassay_004475_15	euxassay_004475_16	euxassay_004475_17	euxassay_004475_18	euxassay_004475_19

euxassay_004475_20	euxassay_004475_21	euxassay_004475_22	euxassay_004475_23	euxassay_004475_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7336_wholemount_strong.wlz

7336_wholemount_moderate.wlz

7336_wholemount_weak.wlz

7336_wholemount_possible.wlz

7336_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7336_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

body cavity or lining

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

limb

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

gland

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

visceral organ system

strong

regional

strong expression: see section 05

alimentary system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

nervous system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

integumental system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

sensory organ system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

cardiovascular system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

urinary system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

reproductive system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

respiratory system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

skeleton

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tail

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tail nervous system

strong

regional

strong expression: see section 01 02

tail skeleton

strong

regional

strong expression: see section 01 02

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6443
Entity Detected:	Tsnax, translin-associated factor X ( MGI:1855672)
Sequence:	sense strand is shown >T6443 GCGCTGTCACCCACGGGCTCAGCGCGGCGTCCCGCGACGGCCTTTCGCTGAGCTGGCCCGGCGGCTGCGA TCCCTGCGCGTGCAAGCCGAGGCCCCGCGGCCATGAACGGCAAAGAAGGACCAGGAGGGTTCAGGAAGAG GAAGCATGACACCTTCCCACATAACCAAAGGAGAGAGGGGAAGGATGCCAGTTTGTCTTCGCCGGTGATG CTGGCCTTCAAATCATTTCAGCAGGAGCTGGACGCCAGGCACGACAAATATGAGAGACTCGTGAAGCTAA GCCGGGATATTACTGTGGAGAGTAAGAGGACAATTTTTCTTCTCCATAGGATTACAAGTGCTCCTGATAT GGAGGAGATACTGACTGAATCAGAAAGTAAGCTGGATGGTGTCAGACAGAAGATCCTGCAGGTAGCCCAA GAGCTGTCGGGGGAAGACATGCACCAGTTCCATCGAGCCGTCACCACAGGACTGCAGGAATACGTGGAGG CTGTCTCTTTTCAACACTTCATCAAAACTCGTTCATTGATCAGTATGGAAGAGATTAACAAGCAGTTGAC ATTTACAGCAGAAGACAGTGGGAAAGAGAGCAAGACTCCTCCCGCGGAAGGACAAGAGAAGCAGCTGGTT ACATGGAGACTGAAGCTCACCCCTGTTGACTACCTGCTGGGCGTGGCCGACCTAACTGGAGAGTTGATGC GGATGTGTATTAACAGTGTGGGGAACGGGGACATCGACACCCCCTTTGAAGTGAGCCAGTTTTTACGCCA GGTTTACGATGGGTTCTCGTTCATTGGCAACACTGGGCCCTATGAGGTCTCTAAGAAGCTGTACACCTTG AAACAGAGCCTGGCCAAAGTGGAGAACGCTTGCTATGCCCTTAAAGTCCGGGGTTCTGAGATTCCTAAGC ACATGCTGGCAGATGTGTTTTCAGTTAAAACAGACATGATTGATCAGGAAGAGAGCATTTCTTAAGCAGC CCCACTCTGCTCTCTAAGGAGCTCCTGGGCGACCAGTTGGTGAGACTGTTCTGAGTGTGCATCTGAGTTT ATTGTGGCTTCTGTAGACGTTCCAAGTTGTACTAGTTTGAAGTTGTAAACAGCTGTATATAAGTTTGTCC ACAGGGAGGTTGTTTCTTACACTCCCGCGCATTTGCATACAGTGTTTGTATATGTGCTCGCTCTATTGTT GTGTGAACGTGCTTCCGATTGCACTTTCTGCCACACCTACTTTAGTTCACTTTGAACTTGGAGTGACATT TTGGGTGGAAGGAAGTCTCTTAAGTGGGTGATTCTGTCTATGGAGGGAAAAGTGTGGAGAAGCTTCCTGC GTGCCTACGTTCCTCAGCAGGATCATGGCAGGCCTTTAACGTGGCTCAGTACGTGAAACAGCAGTTTAAA TATGGAAGTTATCAGTGTTTTCTAAGTGCAAGTTTTGGTGGTGGTATAATGAGAATTCACTTAAAATTCT GGTCAAGTTTAGGTCGTTTTGAATTCGTAGGAAGCAGTTTTCCTGAAGAGTTGATGGGTATCTGGATGTT CACTTTCCCACGTTGTCTTTTCCTTTCTCACCGATGTGGGAAGGAAGGAGCTGACACTGGTCATCACTGG TGACGCTGAGTAACTGACTCCACACTCGTGTGTAGGTTGTGAGCAGCCAGGCCCTTTGTCGTGTGTTAGG TGTGGTGCACTTGTCTACCCCAGCCGTCCTGGGACCCCAGAGGAGCAGCCACAGTGCTCCCAGTGCTGAC AACCCAAGCCTTGACCCTGCCCTTGGTGTGAGCTAGCGATGTTGCCAAAGAAAAAGAATTGTGAGTAATT CAGCAATAAGACGATGGCCAACACTGGAGGGAGCGTCGGGCGTGGGTTCATCTCCCCAGTCAGTCAGAGT CATCTGTGGTGAGAGCCTGGCTTGAGCGTGGCCTGCGGCTGTCAACGCCACATGCTTTCAACGAGTTCTG TGACTTTTCACAACTTAGATACTGGGCTTTATTTTTGGAAAGAATATCTATTTGTAAGGCTGGTTCTTTG AAAGTGTAATTTTCCAAAAAGTCTCACCAAAAGTAAAATAAAGTGTGTACATGCCTGGTAAGTTAGACTT GTTGTCCTGGTGGTGATGAGGCTTCGCCTTCCTGTAGTAAGATGGACTCCTGAGCTGTAGTCAGTCCTGG TACCTGCTCTGTCTGTCTGGTTTTTTTCAGCTACTTCAAGTGGAATTTATAATGTTAAAATAATGGATTG AAGAATGTTTTACAGAAATTTAAAGCAAACTTTAAATAAAATATTAAAATACAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3585400 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3585400 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7335 same embryo
	EMAGE:7333 same embryo
	EMAGE:7332 same embryo
	EMAGE:7334 same embryo
	EMAGE:7337 same embryo
	EurExpress:euxassay_004475 same experiment
	MGI:4828955 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source