EMAGE mouse gene expression spatial database

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EMAGE:7337

Sar1a SAR1 gene homolog A (S. cerevisiae) ( MGI:98230)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004471. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004471_01	euxassay_004471_02	euxassay_004471_03	euxassay_004471_04

euxassay_004471_05	euxassay_004471_06	euxassay_004471_07	euxassay_004471_08	euxassay_004471_09

euxassay_004471_10	euxassay_004471_11	euxassay_004471_12	euxassay_004471_13	euxassay_004471_14

euxassay_004471_15	euxassay_004471_16	euxassay_004471_17	euxassay_004471_18	euxassay_004471_19

euxassay_004471_20	euxassay_004471_21	euxassay_004471_22	euxassay_004471_23	euxassay_004471_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7337_wholemount_strong.wlz

7337_wholemount_moderate.wlz

7337_wholemount_weak.wlz

7337_wholemount_possible.wlz

7337_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7337_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

body cavity or lining

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

limb

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

gland

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

alimentary system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

nervous system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

integumental system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

sensory organ system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

cardiovascular system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

urinary system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

reproductive system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

respiratory system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 20 21 22 23 24

skeleton

strong

regional

strong expression: see section 01 02 18 21 not examined expression: see section 03 04 05 06 07 08 09 10

not examined

regional

not examined expression: see section 01 02 03 04 05 06 07 08 09 10 12

tail nervous system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6442
Entity Detected:	Sar1a, SAR1 gene homolog A (S. cerevisiae) ( MGI:98230)
Sequence:	sense strand is shown >T6442 CCCACGCGTCCGCTGGCGGACCCGGGGACTGCTGGTCTGTGTGCTCTGAGGGAGGGTCGCGCCGCCAGCC ACCGCCGGAGGAGCCCCTCGGGCCGTTGTAAGCATCAATAATGTCTTTCATCTTTGAGTGGATCTACAAT GGCTTCAGCAGTGTGCTCCAGTTCCTAGGACTCTACAAGAAATCTGGAAAACTTGTATTCTTAGGATTGG ACAATGCGGGCAAAACCACTCTTCTTCACATGCTGAAAGATGACAGACTAGGCCAGCACGTACCAACACT CCATCCCACTTCAGAAGAACTGACAATTGCTGGAATGACTTTCACCACTTTTGATCTCGGTGGGCATGAG CAAGCACGTCGCGTTTGGAAAAATTATCTCCCAGCGATTAATGGCATAGTTTTTCTGGTGGACTGTGCGG ATCATTCTCGTCTCATGGAATCCAAAGTTGAGCTTAATGCTTTAATGACTGATGAAACAATATCCAACGT GCCAATTCTTATCTTGGGAAACAAAATTGACAGGACAGATGCAATCAGTGAAGAAAAACTCCGTGAAATA TTTGGGCTGTACGGGCAGACCACAGGAAAGGGGAATGTGACTCTGAAGGAGCTGAACGCCCGCCCCATGG AAGTATTCATGTGCAGTGTGCTCAAGAGGCAAGGCTACGGCGAGGGTTTCCGCTGGCTTTCCCAGTATAT TGACTGATGTTTGGACAGTGAAAATAAAGAGTTTTACTTCTGGACTGATCCTATTCACAGCTTCCTCATG GACTTTTCTAATAGAACAAGGAAAGCTCTCCAACCATGTATGGCATTGAGAAGCCACACGTCTGTCTACG CTCATTGCCCAGTGGTGACATGTGCTCTTCTCCACACTGCCTGCTGTTGCTGCCGGGCAGTGTGTGGGGA CTGGGGCTGGCAGCATTTGCCAGGGAAAGCTGGGTGCCATCATGGGACACCCGAGAAGAAAAACATGTCT CACCACTGGTTGATTTCAAAAGAAAGTGATTCTATTTTTTAAAGAAAGTGTTGTTAATGTAATTGGTATC CCTTCTCTAACTTTTTCAGTTCACAATTTACTTGGTCCAGATTTTCTATTCTTTTTTTTAACTAGTAAAT GAAATTTACATACTTCATAAAATTATGAACAGATAGCGCATTGGGCTCTGCTGTGTGACTGCCTGTTGAG GGTGGTACCCGAGACAAGAGCCTGGAGAGGACAGAGCCTCGGCCTTCCCCAGGGTGGTCTCCACCATGGT AAGGGGAGTCCTCATCTGGAGTGGGTGAAGTATCATGGTCACGTTGTCAACTTTATTTGCAGTTACCAGT TTCAGCAGGCTTTGCTTTTATACTGTTGTGAAATTTCATCTCTCTGTGTAGCACCTTCCTTTTTTCCATT GTAGATATAAAAAGTGACTTTTGCCTCATGAAAGAGTTACAGACATCTGTCATGTAGACCCCATGCTGTG GGTGTGACTGATGCTTTTGAACATAGTCTAGAGGGACGGTTCTGAACCGGAGGGTGCAGCTCAAAATCAT CAGCTTAAGGCTCTGAATTCTGTGCGATTCTCATCTGGCAGCAACAAGAGAACCCAGGAAAAACCCTCAG GGGACTCTGTGTTTTCTCCTTCAGAAATAACAGAAAACCCAGATGGCCTTCTGGCAGCAGCGAGTGCTAA CATGAAAGCAGCAGCGGCCCTGTGAGGATCATGAACAGGCTTTTTATATATATACAGATAGCATTTGGTT TTCTTGCTGCCGTGTGTGATGTTAACTTTATATTTTACCCTTGAGTGCATGAGAGGAGATGGTCTGCAGA TGGGAGGGTATCTTGATTCTGATTTCCTTTAATGCAAGGTGAGAGTAGGTTCTTAAGTGGAGATTCTGCT GTTGCTGCTGCTGTATTCTGACAGGCTCGTGTCCAGCAAGAGGGTAGGGAAGCTAGAAGCACACTCCTGG AGAGTGACTTACCACATTTTAGTTTTGAGCACTGAGCCTATGGGACAGTGTTTTGTTACACTGCCGGGTT TCCAATGCAGAGGCTTGGTGAGGTGTCTGTCTGCAAACTGCATGCACTGCGTTCACCCACGTACAGCTGG GATGAGAACTGTCAGTGTGGAAGGGCCTACTGTTAAAGCCCTGAGTGAGCTAGGTAGGTGACTGTTGTGC CCTTCATTGCCTTTGGCTAAAGACTGTCCACATGTCTGTCCAGGCACATGACAGGTGTGAGGGCCTCAGC GTTGGGTTCTTCATCTGTCTTTCTCGTTTGTCCCTAATTGCTCGGTAGCAACCTAGAAGACTGGACTCTG CCCAGGGTGGCTCTTTGTGAAAGTGGTTTATTCTGGCCACCGGAGAAAGATGGCTGAACAGTGGCACCAG TCTGCTTCATGCTGCACAGAACACCGTCTTAAGTAAGGTGGCCAAAAGAGGTTGCTGCAGCAAGTGCAAC TCTGCCAGGGCCATGGCCAGGGGCTCCAATGAACTGAGACTCTTCATTCTGTTTATCAGAGTGCACATGT GTCCTGTTTCAGCAAAAGTAAAACTGTCATTTACAAAAGGTCAGTCTGTATCCTAAGCATTTTAATAAAA AGCTAAAATGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3585667 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3585667 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7335 same embryo
	EMAGE:7333 same embryo
	EMAGE:7332 same embryo
	EMAGE:7334 same embryo
	EMAGE:7336 same embryo
	EurExpress:euxassay_004471 same experiment
	MGI:4827869 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source