Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7371

Polr2d polymerase (RNA) II (DNA directed) polypeptide D ( MGI:1916491)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7371 EMAGE:7371 EMAGE:7371 EMAGE:7371 EMAGE:7371
"Pseudo-wholemount" of euxassay_004497. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004497_01 euxassay_004497_02 euxassay_004497_03 euxassay_004497_04
EMAGE:7371 EMAGE:7371 EMAGE:7371 EMAGE:7371 EMAGE:7371
euxassay_004497_05 euxassay_004497_06 euxassay_004497_07 euxassay_004497_08 euxassay_004497_09
EMAGE:7371 EMAGE:7371 EMAGE:7371 EMAGE:7371 EMAGE:7371
euxassay_004497_10 euxassay_004497_11 euxassay_004497_12 euxassay_004497_13 euxassay_004497_14
EMAGE:7371 EMAGE:7371 EMAGE:7371 EMAGE:7371 EMAGE:7371
euxassay_004497_15 euxassay_004497_16 euxassay_004497_17 euxassay_004497_18 euxassay_004497_19
EMAGE:7371 EMAGE:7371 EMAGE:7371 EMAGE:7371 EMAGE:7371
euxassay_004497_20 euxassay_004497_21 euxassay_004497_22 euxassay_004497_23 euxassay_004497_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7371Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7371_wholemount_strong.wlz
7371_wholemount_moderate.wlz
7371_wholemount_weak.wlz
7371_wholemount_possible.wlz
7371_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7371_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6481
Entity Detected:Polr2d, polymerase (RNA) II (DNA directed) polypeptide D ( MGI:1916491)
Sequence:sense strand is shown

>T6481
CGGCAGGTGGAAGCGATCCTCGGGCTGGCGACGTAGAAGAAGATGCTTCCCAGCTTATCTTTCCCAAAGA
GTTTGAGACAGCGGAGACTCTGCTGAACTCGGAAGTCCACATGCTTCTGGAGCATCGAAAGCAGCAGAAC
GAGAGCGCGGAGGACGAGCAGGAGCTGTCGGAGGTCTTCATGAAAACCCTCAACTACACGGCCCGCTTCA
GCCGCTTCAAAAACAGAGAGACCATTGCCAGCGTGCGCAGCTTGCTTCTCCAGAAAAAGTTACATAAGTT
TGAGTTGGCCTGTTTAGCCAATCTTTGCCCGGAGACTGCTGAAGAGTCCAAAGCTCTGATTCCAAGCCTG
GAAGGGCGTTTTGAAGATGAGGAGCTGCAGCAGATTCTCGATGACATCCAGACGAAGCGCAGCTTCCAGT
ACTGATCCGCAGACGTCACTCTGCTCCTTGAGTTACAGCACCACGGTCTGAAGCCCGGGGAGGCCTCATG
CAGGAACATTGCTTCAGAAGACGCTGGAGCTTCTTTCGTAGTTTTTGCTTCACTGCGAGAGAGTGTCTCT
CCAGTTCTCAAAGTCATAACAGTCCTCCTGCCTCAGCCTCGCAGGTGCTGGGATTACAGGTGTGAGCCAA
CACTTTCAGCTTGGCTTCTGGAGAGGAAAGAAAGGCTGGCTATTGATGCTGTAGGTGCTATCTTCACAGT
GACTGTTAGACCATAACTACCATCTGCGCTCCTTGCAGCCTTTTAAGGCTGCTGTGGTCAGGCAGGCTGC
CATCCTGAACTGCTGAGGTAGAATGATGTTCAAACATTCGTTGACTACTTCGTACCGTGGTTTTTTTGGA
CCCCATGTTACTATTTTTTTGTTCTTGACACTTCAGATTTTAATGTCAATTATTTTGTAGTTTTTGAAGC
TAAAATGATATATAAAAAACTTGCATAGGAGAGGAGTGTTTTCACTTGGTTGTAAAGGGGAGAAATAAAC
CAGAACACTGTTTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3588284 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3588284 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7375 same embryo
 EMAGE:7374 same embryo
 EMAGE:7373 same embryo
 EMAGE:7372 same embryo
 EMAGE:7370 same embryo
 EurExpress:euxassay_004497 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS