EMAGE mouse gene expression spatial database

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EMAGE:7389

Aida axin interactor, dorsalization associated ( MGI:1919737)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004513. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004513_01	euxassay_004513_02	euxassay_004513_03	euxassay_004513_04

euxassay_004513_05	euxassay_004513_06	euxassay_004513_07	euxassay_004513_08	euxassay_004513_09

euxassay_004513_10	euxassay_004513_11	euxassay_004513_12	euxassay_004513_13	euxassay_004513_14

euxassay_004513_15	euxassay_004513_16	euxassay_004513_17	euxassay_004513_18	euxassay_004513_19

euxassay_004513_20	euxassay_004513_21	euxassay_004513_22	euxassay_004513_23	euxassay_004513_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7389_wholemount_strong.wlz

7389_wholemount_moderate.wlz

7389_wholemount_weak.wlz

7389_wholemount_possible.wlz

7389_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7389_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5754
Entity Detected:	Aida, axin interactor, dorsalization associated ( MGI:1919737)
Sequence:	sense strand is shown >T5754 GGGGTCCGTAGGCCTCGCCGAGCCTCCCTTTCCTCACCACTGCGTACTAGGGCCCGAAGCCCGGGGCGGG GACCGGCCATGTCGGAGGTGACCCGGAGTCTGCTGCAGCGCTGGGGCGCCAGTCTTAGGAGAGGCGCCGA CTTCGACTCTTGGGGCCAGCTGGTGGAGGCGATAGACGAGTATCAGATATTAGCAAGACACTTACAGAAG GAGGCCCAAGCTCAACACAATAATTCTGAATTTACAGAAGAACAAAAGAAAACCATAGGCAAAATTGCGA CATGTTTGGAATTGCGTAGTGCAGCATTACAGTCCACACAGTCCCAAGAAGAGTTTAAACTGGAGGACCT GAAGAAGCTAGAACCAATCCTGAAGAATATTCTTACATATAATAAAGAATTCCCATTTGATGTTCAGCCT ATTCCATTAAGACGTATTTTAGCACCTGGTGAAGAAGAGAATTTGGAATTTGAAGAAGATGAAGAAGGTG GTGCTGGAGCAGGGCCTCCTGATTCTTTCTCTGCTAGAGTTCCTGGTACTTTATTGCCACGGTTGCCATC TGAACCAGGGATGACATTACTCACCATCAGGATTGAGAAAATTGGTTTGAAAGATGCGGGGCAGTGCATC GATCCCTACATCACAGTCAGCGTGAAAGATTTGAATGGCATAGATTTAACTCCCGTACAAGACACTCCTG TGGCTTCAAGGAAAGAAGATACTTACGTCCACTTTAATGTGGACATTGAGCTCCAGAAACACGTTGAAAA GTTAACCAAAGGTGCGGCAATTTTCTTTGAATTCAAACATTACAAGCCTAAAAAGAGGTTTACCAGCACT AAGTGTTTTGCCTTCATGGAGATGGATGAAATTAAACCTGGGCCAATCGTAATAGAACTATACAAGAAAC CCACTGACTTTAAAAGAAAGAAATTGCAATTGTTGACCAAGAAACCACTTTATCTTCATCTACATCAAAG TTTGCACAAGGAATGATCCTGATACAAATAACCAAGAACTTGTGTGAATTTTACCACTCAGAACCCATCA TAGCTCTGAGTAGCATAATCACCCTTCAACAGGCAGGAAGTGAGCCCTACCCATGCCAGCTGACCAAGCC AGTCTATTGCACAGCCGGACCCCAGAACAAAAGTTCAGCACATCACTGACCTATCACTCCTTTGGATACA GGTTTATTGTAGATTTGGAAACGTTTTCACTTTTCTATTAATTGTGCAATGAATAGTCTATTTTCTCATT GCCACTACTCCCCTGTTTCCCGGACAGCACTGTTGTAGATGTGCAGAACTTCAACAATGCAGCAATAAGA ATGTGCAAGAGTCTACGTCTCTGCTCACTTCTGCTCCAGCATGCTCAATTTCAATGCCATGCTGTGAGTT GAAGTGGGTAGAACTGCTCTTAGAGGAACTGGACTAGTTACATTGCCTAGCAAAGTCTCCTTAGAAGAGA CCTAGCCTGGTGCCGTGGGTCTCCTGCATAGAAAGTGTGACCTGGCCAGATGAGCATCCCACCCTGAGTG TTCCCCGGATGACCAGACTGATTTCCTGAAGAGAGCACGTGTATCGTCAGGTGGATTATCTGCTCTTCTC TATTGTACTGCTAATTCTTTCTGGTAGTTCTAGGGTTAACGTTGGAACCATCTCAGAATAACTATGGATT TGAAAATGTGTTTACAGTATCTTTTAAACTGTATCATTTAGGAACAGTTGAGTCAGATGTCAAGATGTTG CGGGATAACTGCTGCTTTCCTGACCACCCGTTGTGAGACCTTAAGACCCCGTACCTCTCCTTAGTATGCA AAAATCTGGAAAGACTGTCTATTTTTTTTTAATATAGGTAGATAGGATTGCCATTTATTTCCTATTTAGA TATACAGTCATTCATCCATATGAAAATATGCAGGTCACTAGTTTATTTTGAATGGACTATTTACTTTTGG TGTAACAAGGCATAAATAAAACTTTGAAAGCACAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2651163 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2651163 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7390 same embryo
	EMAGE:7391 same embryo
	EMAGE:7392 same embryo
	EMAGE:7393 same embryo
	EMAGE:7388 same embryo
	EurExpress:euxassay_004513 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source