EMAGE mouse gene expression spatial database

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EMAGE:7390

Atp6v1a ATPase, H+ transporting, lysosomal V1 subunit A ( MGI:1201780)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004518. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004518_01	euxassay_004518_02	euxassay_004518_03	euxassay_004518_04

euxassay_004518_05	euxassay_004518_06	euxassay_004518_07	euxassay_004518_08	euxassay_004518_09

euxassay_004518_10	euxassay_004518_11	euxassay_004518_12	euxassay_004518_13	euxassay_004518_14

euxassay_004518_15	euxassay_004518_16	euxassay_004518_17	euxassay_004518_18	euxassay_004518_19

euxassay_004518_20	euxassay_004518_21	euxassay_004518_22	euxassay_004518_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7390_wholemount_strong.wlz

7390_wholemount_moderate.wlz

7390_wholemount_weak.wlz

7390_wholemount_possible.wlz

7390_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7390_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 06 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 07 16

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5760
Entity Detected:	Atp6v1a, ATPase, H+ transporting, lysosomal V1 subunit A ( MGI:1201780)
Sequence:	sense strand is shown >T5760 GTCTAGGTACACTTTCTGCATTTCGAGCCCGGGCAGGTAAATTTAACACAATGGATTTCTCCAAGCTACC CAAAATCCGCGATGAGGATAAAGAAAGTACATTTGGTTATGTGCATGGAGTCTCAGGGCCTGTGGTTACA GCCTGTGACATGGCGGGCGCTGCCATGTACGAGCTGGTGAGAGTGGGGCACAGCGAGCTGGTTGGAGAAA TTATTCGATTGGAAGGTGACATGGCCACCATTCAGGTGTATGAAGAGACTTCTGGTGTCTCTGTTGGAGA CCCCGTACTCCGCACTGGTAAACCTCTCTCGGTCGAGCTGGGTCCCGGGATTATGGGAGCCATTTTTGAT GGTATACAGAGACCTCTGTCGGATATCAGCAGTCAGACCCAAAGTATCTACATCCCCAGAGGAGTCAATG TGTCTGCTCTCAGCAGAGATATCAAATGGGAGTTTATACCCAGCAAAAACCTACGGGTTGGTAGTCATAT CACTGGTGGAGACATTTATGGGATTGTCAATGAGAACTCCCTCATCAAACACAAAATCATGTTGCCCCCA CGTAACAGAGGAAGCGTGACTTACATCGCGCCGCCTGGGAATTATGATGCGTCCGATGTCGTCCTGGAGC TTGAGTTTGAAGGTGTGAAGGAGAAGTTCAGCATGGTCCAAGTGTGGCCTGTGCGGCAGGTGCGGCCTGT CACTGAGAAGCTGCCCGCCAATCACCCCTTGCTTACTGGCCAGAGAGTCCTCGATGCCCTTTTCCCGTGT GTTCAGGGAGGAACTACTGCTATCCCAGGCGCCTTTGGCTGTGGAAAGACTGTGATTTCCCAGTCTCTAT CCAAGTACTCCAACAGTGACGTCATCATCTATGTCGGCTGCGGTGAGAGAGGCAACGAGATGTCAGAAGT TCTCCGCGACTTCCCTGAGCTCACCATGGAGGTTGATGGGAAAGTAGAGTCCATCATGAAGAGGACAGCG CTGGTAGCCAACACCTCCAACATGCCTGTGGCTGCAAGAGAGGCCTCCATCTACACTGGAATTACACTAT CAGAATATTTCCGTGACATGGGCTACCACGTCAGTATGATGGCGGACTCTACCTCTAGATGGGCTGAGGC CCTCAGAGAAATCTCTGGTCGCTTAGCTGAGATGCCTGCAGATAGTGGATACCCTGCATACCTTGGTGCC CGGCTGGCTTCTTTCTATGAGCGAGCAGGCAGAGTGAAATGTCTTGGAAACCCTGAGAGAGAAGGGAGTG TCAGCATTGTAGGAGCAGTTTCTCCACCTGGTGGTGATTTTTCTGATCCAGTCACTTCTGCAACTCTGGG TATTGTTCAGGTGTTCTGGGGCTTGGATAAGAAGTTAGCTCAGCGCAAGCACTTCCCCTCTGTCAACTGG CTCATCAGCTACAGCAAGTACATGCGTGCCCTGGACGAGTACTATGACAAGCACTTCACGGAGTTTGTTC CTCTGAGGACCAAAGCTAAGGAGATCCTGCAGGAAGAAGAGGATCTGGCAGAAATCGTGCAGCTCGTGGG AAAGGCCTCTTTAGCAGAGACGGATAAAATCACTCTGGAGGTAGCAAAACTTATTAAAGATGACTTCCTA CAGCAAAATGGGTACACTCCTTATGACAGGTTCTGTCCATTCTACAAGACAGTGGGGATGCTGTCCAACA TGATTTCATTCTATGACATGGCCCGCCGAGCTGTGGAGACCACTGCCCAGAGTGACAATAAGATCACATG GTCCATTATCCGGGAGCACATGGGGGAGATTCTCTATAAACTCTCCTCCATGAAATTCAAGGATCCAGTG AAAGATGGCGAGGCAAAGATCAAGGCCGACTATGCACAGCTTCTTGAAGATATGCAGAATGCATTCCGTA GCCTTGAAGACTAGAACTGTGATTTCTTCCCTCTTCCACGGCAAGCTCATACGTGTATATTTTCCTGAAT TTCTCATCTCCAACCCTTAGCTTCCATATTGTGCAGCTTTGAGACTAGTGCCTATGTGTGTTCTTGTTCA TTTTGCTGTTTCTTTGGTAGGTCTTATAAAACAAACATTCCTTTGTTCCAGTGTTTGAAGGAGCTAGCTC CCTTACCTTTATCTGAAGTGATGAATGTAGTGCATATGATATACATTGTAAGATACACATTGTAACATGA TCCATACTGTAAACTTGTATGTAAGGTGACTACCCCTTCCCTCATCTCCAGTAAACTGTAAACAGGACTA CTGCATGTACTCTGTTGGGAATGGAAGGCCAGAACTCCATACCGTGGATGGATGGGTACTTAGGAAACAA CTCAGCATTTGTAGTCAGACCACTTGTAACTTAGTGGTTTGTTGAGTAACCATTTTGCAGGAAATACTTC CATTTAAAAACATAAAAGATTAATGTTCCAATTATTTGTATCAATCAGGACCATTTTTGTGGGGCACTTG GGAACTATTTGTTTTTCAAACAGACATTTGCAAGACTGAACATAATAGATAAATCAGTTACCTCTGAAAA TGTGAAAGAAAGGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2651677 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2651677 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7389 same embryo
	EMAGE:7391 same embryo
	EMAGE:7392 same embryo
	EMAGE:7393 same embryo
	EMAGE:7388 same embryo
	EurExpress:euxassay_004518 same experiment
	MGI:4823325 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source