EMAGE mouse gene expression spatial database

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EMAGE:7391

H2-Ab1 histocompatibility 2, class II antigen A, beta 1 ( MGI:103070)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004525. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004525_01	euxassay_004525_02	euxassay_004525_03	euxassay_004525_04

euxassay_004525_05	euxassay_004525_06	euxassay_004525_07	euxassay_004525_08	euxassay_004525_09

euxassay_004525_10	euxassay_004525_11	euxassay_004525_12	euxassay_004525_13	euxassay_004525_14

euxassay_004525_15	euxassay_004525_16	euxassay_004525_17	euxassay_004525_18	euxassay_004525_19

euxassay_004525_20	euxassay_004525_21	euxassay_004525_22	euxassay_004525_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7391_wholemount_strong.wlz

7391_wholemount_moderate.wlz

7391_wholemount_weak.wlz

7391_wholemount_possible.wlz

7391_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7391_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5756
Entity Detected:	H2-Ab1, histocompatibility 2, class II antigen A, beta 1 ( MGI:103070)
Sequence:	sense strand is shown >T5756 GGGGCACAGCAGGTGTGAGTCCTGGTGACTGCCATTACCTGTGCCTTAGAGATGGCTCTGCAGATCCCCA GCCTCCTCCTCTCGGCTGCTGTGGTGGTGCTGATGGTGCTGAGCAGCCCAGGGACTGAGGGCGGAGACTC CGAAAGGCATTTCGTGGTCCAGTTCAAGGGCGAGTGCTACTACACCAACGGGACGCAGCGCATACGGCTC GTGACCAGATACATCTACAACCGGGAGGAGTACGTGCGCTACGACAGCGACGTGGGCGAGTACCGCGCGG TGACCGAGCTGGGGCGGCCAGACGCCGAGTACTGGAACAGCCAGCCGGAGATCCTGGAGCGAACGCGGGC CGAGGTGGACACGGCGTGCAGACACAACTACGAGGAGACGGAGGTCCCCACCTCCCTGCGGCGGCTTGAA CAGCCCAATGTCGCCATCTCCCTGTCCAGGACAGAGGCCCTCAACCACCACAACACTCTGGTCTGTTCGG TGACAGATTTCTACCCAGCCAAGATCAAAGTGCGCTGGTTCAGGAATGGCCAGGAGGAGACAGTGGGGGT CTCATCCACACAGCTTATTAGGAATGGGGACTGGACCTTCCAGGTCCTGGTCATGCTGGAGATGACCCCT CATCAGGGAGAGGTCTACACCTGCCATGTGGAGCATCCCAGCCTGAAGAGCCCCATCACTGTGGAGTGGA GGGCACAGTCCGAGTCTGCCCGGAGCAAGATGTTGAGCGGCATCGGGGGCTGCGTGCTTGGGGTGATCTT CCTCGGGCTCGGCCTTTTCATCCGTCACAGGAGTCAGAAAGGACCTCGAGGCCCTCCTCCAGCAGGGCTC CTGCAGTGACTCAGAGTGTTTTGACTCAGTTGACTGTCTCAGACTGTAAGGCCTGCATGTCTCTGCTTCG AATTCCTGACTGCCAGTCTACCTGCCACTCCGACTCAGAGTCTAGCATGGCACTATTGTATCCACCACCT CAGCTCTTGTGATCTGGAGTCCCCCAGTCTCTGTCTGTAGCTCTGCTCCTTGGTGATTCCAGAGACTCCA TCTGTGTACAGCAGCACCCCAGGCTTTCTGTCTTCGCTCTGTAGTAAACCAATGTATGCTTATCCCCACC TAGATTACAAATAAACGAGACCCAGACTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2651058 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2651058 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7389 same embryo
	EMAGE:7390 same embryo
	EMAGE:7392 same embryo
	EMAGE:7393 same embryo
	EMAGE:7388 same embryo
	EurExpress:euxassay_004525 same experiment
	MGI:4825302 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source