EMAGE mouse gene expression spatial database

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EMAGE:7400

Ugt2b38 UDP glucuronosyltransferase 2 family, polypeptide B38 ( MGI:2140794)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004449. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004449_01	euxassay_004449_02	euxassay_004449_03	euxassay_004449_04

euxassay_004449_05	euxassay_004449_06	euxassay_004449_07	euxassay_004449_08	euxassay_004449_09

euxassay_004449_10	euxassay_004449_11	euxassay_004449_12	euxassay_004449_13	euxassay_004449_14

euxassay_004449_15	euxassay_004449_16	euxassay_004449_17	euxassay_004449_18	euxassay_004449_19

euxassay_004449_20	euxassay_004449_21	euxassay_004449_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7400_wholemount_strong.wlz

7400_wholemount_moderate.wlz

7400_wholemount_weak.wlz

7400_wholemount_possible.wlz

7400_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7400_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

kidney calyx

strong

regional

strong expression: see section 06 15 16 moderate expression: see section 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7563
Entity Detected:	Ugt2b38, UDP glucuronosyltransferase 2 family, polypeptide B38 ( MGI:2140794)
Sequence:	sense strand is shown >T7563 CCCACGCGTCCGGGATTTTGATTTTCAGGATGCCTGGAAAGTGGATTTCTGCTCTGCTGCTGCTGCAGAT AAGTTGCTGCTTCAGATCTGTGAAATGTGGGAAAGTGTTGGTGTGGCCGATGGAATTCAGTCATTGGATG AACATAAAAATAATACTGGATGAACTGGTACAGAGAGGCCATGAAGTCACTGTTCTGAGACCTTCAGCTT ACTATGTTCTTGATCCCAAAAAATCACCTGGCCTTAAGTTTGAAACTTTTCCTACATCTGTCAGTAAAGA CAATCTGGAAAATTTTTTCATTAAGTTTGTGGATGTGTGGACTTATGAAATGCCAAGGGATACATGTTTG TCATATTCTCCTTTACTGCAAAATATGATTGATGAATTTTCTGATTATTTTCTAAGTCTTTGTAAAGATG TTGTTTCAAACAAAGAGCTCATGACAAAGCTACAGGAATCCAAGTTTGATGTGCTTTTGTCAGATCCTGT TGCTTCCTGTGGGGAGCTCATAGCTGAACTGCTCCAGATTCCTTTTCTGTACAGTATCCGCTTCTCTCCA GGCTACCAAATTGAAAAGTCCAGCGGGAGATTTCTACTCCCTCCCTCTTATGTACCTGTAATTCTGTCAG GATTAGGTGGACAAATGACATTCATAGAGAGAGTAAAAAATATGATATGCAGGCTTTATTTTGACTTTTG GTTCCAGATGTTTAATGATAAGAAATGGGACTCATTTTACAGTGAATATTTGGGAAGGCCCACCACCTTA GCTGAGACAATGGGCAAGGCAGAAATGTGGCTCATTAGATCCAACTGGGATTTGGAGTTTCCTCACCCAA CCTTACCAAATGTTGACTATGTTGGAGGACTCCACTGCAAACCTGCTAAACCTTTGCCTAAGGATATGGA AGAATTTGTCCAGAGCTCTGGAGACCATGGTGTGGTGGTGTTTTCACTTGGGTCAATGGTTAGTAACATG ACAGAAGAAAAAGCCAACACAATTGCATGGGCCCTTGCCCAGATTCCACAAAAGGTTCTTTGGAAATTTG ATGGCAAAACCCCAGCAACTTTAGGACACAATACGAGAGTGTACAAGTGGCTCCCCCAGAATGACCTCCT TGGTCATCCAAAAACAAAAGCCTTTGTAACTCATGGTGGGGCCAATGGTGTCTATGAGGTGATCTATCAT GGAATCCCTATGATTGGCATTCCTTTGTTTGGAGAACAACATGATAATATTGCCCATATGGTGGCCAAAG GAGCAGCTGTTACATTGAATATCAGAACAATGTCAAGATCAGATGTACTCAATGCACTGGAGGAAGTCAT AGACAATCCTTTCTATAAAAAGAATGCTATATGGTTGTCAACCATTCACCACGACCAGCCTACGAAGCCC CTGGACAGGGCTGTCTTCTGGGTTGAGTTTGTCATGCGCCACAAAAGGGCTAAGCACCTGAGATCACTTG GACATAACCTTACCTGGCACCAGTACCACTTTCTAGATGTGATCGGCTTCCTACTCTCTTGTGTTGCAGT CACTATAGTTCTTACTGTAAAGTGCCTCTTGTTCATTTACCGATTCTTTGTAAAGAAAGAAAAGAAAATT AAGAATGAGTAGAGCTCATTGACAATGGACTACATGAATGAACTTTCACCCTCATTCTAATTTATGAACC ACCTTCTAAATACTGATTTGATTTTTTTTTAATCAAGGCAGATCTTCTTTGGAAATTTTTACTGTGTAAG AAGACATGTAAATCTGTGGATACTGATCCATTTTCAAAAATCTCATATTTTTTTTAAATTTCAAACTATT TAATATAAAAAATTGTATTGTAGAAAAATGTGAAAAATAAAGATGTACTTGATAGAGTCTTAAAAATCAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4241480 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4241480 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7404 same embryo
	EMAGE:7405 same embryo
	EMAGE:7401 same embryo
	EMAGE:7402 same embryo
	EMAGE:7403 same embryo
	EurExpress:euxassay_004449 same experiment
	MGI:4829090 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source