EMAGE mouse gene expression spatial database

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EMAGE:7402

Gipc1 GIPC PDZ domain containing family, member 1 ( MGI:1926252)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004534. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004534_01	euxassay_004534_02	euxassay_004534_03	euxassay_004534_04

euxassay_004534_05	euxassay_004534_06	euxassay_004534_07	euxassay_004534_08	euxassay_004534_09

euxassay_004534_10	euxassay_004534_11	euxassay_004534_12	euxassay_004534_13	euxassay_004534_14

euxassay_004534_15	euxassay_004534_16	euxassay_004534_17	euxassay_004534_18	euxassay_004534_19

euxassay_004534_20	euxassay_004534_21	euxassay_004534_22	euxassay_004534_23	euxassay_004534_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7402_wholemount_strong.wlz

7402_wholemount_moderate.wlz

7402_wholemount_weak.wlz

7402_wholemount_possible.wlz

7402_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7402_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5806
Entity Detected:	Gipc1, GIPC PDZ domain containing family, member 1 ( MGI:1926252)
Sequence:	sense strand is shown >T5806 GAGAAGCCGGGCGGAGTTTCGAGAGGACCGAGCAGGGCGCGCGCGGTGGCGGCGGCGCAGGTCTCCCAGC CAGAGTCCCCACTTCTGGCTGCTCATGCCACTGGGACTGGGGCGAAGGAAGAAAGCACCGCCTCTGGTGG AAAATGAGGAGGCGGAGCCAAGCCGGAGTGGGCTGGGTGTTGGGGAGCCTGGGCCCCTGGGTGGGAGCGC AGCAGGAGAATCCCAGATGGGCTTGCCCCCACCTCCTGCTGCCCTCCGGCCTCGCCTCGTGTTCCATACC CAGCTGGCCCACGGCAGCCCCACAGGCCGCATCGAGGGCTTCACTAATGTCAAGGAGCTGTACGGCAAGA TCGCTGAGGCCTTCCGACTACCAGCTGCTGAGGTGATGTTCTGCACCCTCAACACCCACAAAGTGGACAT GGACAAACTCCTGGGGGGCCAGATCGGCCTGGAAGACTTCATCTTTGCCCATGTGAAGGGGCAGCGCAAA GAGGTGGAAGTGTTCAAGTCGGAGGAGGCTCTGGGGCTCACCATCACCGACAACGGGGCCGGCTACGCCT TCATTAAGCGCATCAAGGAAGGCAGTGTGATTGACCACATTCAGCTCATCAGCGTGGGGGACATGATTGA AGCCATCAACGGGCAGAGCCTGCTGGGCTGTCGGCATTACGAGGTTGCCAGGCTCCTCAAGGAGCTGCCC CGAGGCCGCACCTTCACCCTCAAACTCACAGAACCTCGAAAGGCCTTTGATATGATCAGCCAGCGTTCAG CCGGTGGCCACCCTGGTTCAGGCCCACAACTGGGCACTGGCCGAGGGACCCTCCGTCTCCGATCCCGGGG CCCTGCCACCGTGGAGGATCTGCCGTCGGCCTTTGAGGAGAAGGCCATTGAGAAGGTGGATGACTTGCTA GAGAGCTACATGGGGATCAGAGACACGGAGCTGGCTGCCACCATGGTGGAGCTGGGAAAGGACAAAAGGA ACCCGGACGAGCTGGCAGAAGCCCTGGATGAGCGGCTCGGTGACTTCGCATTCCCAGATGAATTTGTCTT TGATGTCTGGGGAGCTATCGGGGATGCCAAGGTTGGCCGCTACTAAGACTGGAACTGAACCTGGACCTTG AGATAACCTGGCTTGGCCTGGCTGGGGTTTCCAGGAGGGGTGCCACAGCCGGGACCTGGGCACACAGCCG AGGCTCGTAAATCAGCAGCCTGGGAAAGGCATGGGGTGCAAATGCCCTGTGGTCCTGGCCCCACTCCAAT CAGTACCACCACGGTGCCCAGCCTGGTTGGGGTCCCCGGCCAACCCCTGCCGGGTTCCCCACCTACCTCA ATAGAGTACTCGCATGGGAGGGACCAACACACTGGGCAGTCACCTCTGTCCCCAACATTTCCCACCATCA GCTGGCAACTGCCCCTTCTGTCCCCCTGGGCCTCAGTTCTCTTTGGGGTCATGACCTCCCTACTTTACTT TTTTTGCTTTGTTTATTTTCAAGACAAAGTCACTATGTAGCTCTGGCTGTCCTGGAACTCATTCTGTAGA CCAGGCTGGCCTTGAACTCACAGAGATCCACCTGCATCTGCATTCACAGTGCTGGGATTAAAGGTGTGCG TCACCAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2811749 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2811749 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7404 same embryo
	EMAGE:7405 same embryo
	EMAGE:7400 same embryo
	EMAGE:7401 same embryo
	EMAGE:7403 same embryo
	EurExpress:euxassay_004534 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source