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EMAGE:7403

Tnmd tenomodulin ( MGI:1929885)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7403 EMAGE:7403 EMAGE:7403 EMAGE:7403 EMAGE:7403
"Pseudo-wholemount" of euxassay_004572. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004572_01 euxassay_004572_02 euxassay_004572_03 euxassay_004572_04
EMAGE:7403 EMAGE:7403 EMAGE:7403 EMAGE:7403 EMAGE:7403
euxassay_004572_05 euxassay_004572_06 euxassay_004572_07 euxassay_004572_08 euxassay_004572_09
EMAGE:7403 EMAGE:7403 EMAGE:7403 EMAGE:7403 EMAGE:7403
euxassay_004572_10 euxassay_004572_11 euxassay_004572_12 euxassay_004572_13 euxassay_004572_14
EMAGE:7403 EMAGE:7403 EMAGE:7403 EMAGE:7403 EMAGE:7403
euxassay_004572_15 euxassay_004572_16 euxassay_004572_17 euxassay_004572_18 euxassay_004572_19
EMAGE:7403 EMAGE:7403 EMAGE:7403 EMAGE:7403 EMAGE:7403
euxassay_004572_20 euxassay_004572_21 euxassay_004572_22 euxassay_004572_23 euxassay_004572_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7403Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7403_wholemount_strong.wlz
7403_wholemount_moderate.wlz
7403_wholemount_weak.wlz
7403_wholemount_possible.wlz
7403_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7403_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 21 22 23 24 moderate expression: see section 01 02 03 04 05 06 07 08 09 17 18 19 20
diaphragm
strong strong
regionalstrong expression: see section 10 11 13 14 15 17 moderate expression: see section 12
humerus
strong strong
regionalstrong expression: see section 23 24 moderate expression: see section 01 02 03 04 05 21 22
hand mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 04
hindlimb digit 1 phalanx
strong strong
regionalstrong expression: see section 19
hindlimb digit 2 phalanx
strong strong
regionalstrong expression: see section 18 19
hindlimb digit 3 phalanx
strong strong
regionalstrong expression: see section 01 17 18 19
hindlimb digit 4 phalanx
strong strong
regionalstrong expression: see section 01 17 18 19
hindlimb digit 5 phalanx
strong strong
regionalstrong expression: see section 01 02 17 18 19
foot mesenchyme
strong strong
regionalstrong expression: see section 02 moderate expression: see section 20 21
lower leg mesenchyme
moderate moderate
regionalmoderate expression: see section 20 22
femur
moderate moderate
regionalmoderate expression: see section 04 05 18 19 weak expression: see section 03
trunk mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 14 15 16 17 18 19 20 21 22 23 24
exoccipital bone
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 23 24 moderate expression: see section 07 22
temporal bone
strong strong
regionalstrong expression: see section 03 23 24
vault of skull
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 23 24 moderate expression: see section 07 22
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 23 24 moderate expression: see section 22
sternum
strong strong
regionalstrong expression: see section 12 13 15 16 moderate expression: see section 10 11 14 17 18
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 17 moderate expression: see section 07 08 14 15 16
tail mesenchyme
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5807
Entity Detected:Tnmd, tenomodulin ( MGI:1929885)
Sequence:sense strand is shown

>T5807
CATATATGGGTATATCTATGTAACAAATCGTAGCACGGGAGCCCCCTGGGCTCCCTCCGGCTCTGGTATG
ACATATTTGAGCCATATAAATTCAGCTTCTCCTCCGGCATCTGGTAGCCGACTCACTTGCAACTCCACCT
CAGCAGTAGTCCTCTCAGTCCTCTCAAAGCAGGGAAAGAGCACCGTGTGCTGGGAGACCATGGCAAAGAA
TCCTCCAGAGAACTGTGAGGGCTGTCACATTCTAAATGCAGAAGCTCTGAAATCTAAGAAGATATGTAAA
TCACTGAAGATTTGTGGACTAGTGTTTGGTATCCTGGCCTTAACTCTAATTGTCCTGTTTTGGGGGAGCA
AACACTTCTGGCCCGAGGTATCCAAGAAAACCTATGACATGGAGCACACTTTCTACAGCAACGGCGAGAA
GAAGAAGATTTACATGGAAATTGATCCCATAACCAGAACAGAAATATTCAGAAGTGGAAATGGCACTGAT
GAAACATTGGAAGTCCATGACTTTAAAAATGGATACACTGGCATCTACTTTGTAGGTCTTCAAAAATGCT
TTATTAAAACTCAAATCAAAGTGATTCCTGAATTTTCTGAACCAGAGGAAGAAATAGATGAGAATGAAGA
AATTACTACAACTTTCTTTGAACAGTCAGTGATTTGGGTTCCCGCAGAAAAGCCTATTGAAAACAGAGAC
TTCCTGAAAAATTCTAAAATTCTGGAGATTTGCGATAATGTGACCATGTACTGGATCAATCCCACTCTAA
TAGCAGTTTCAGAATTACAGGACTTTGAGGAGGACGGTGAAGATCTTCACTTTCCTACCAGTGAAAAAAA
GGGGATTGACCAGAATGAGCAATGGGTGGTCCCGCAAGTGAAGGTGGAGAAGACCCGCCACACCAGACAA
GCAAGCGAGGAAGACCTTCCTATAAATGACTATACTGAAAATGGAATTGAATTTGACCCAATGCTGGATG
AGAGAGGTTACTGTTGTATTTACTGTCGTCGAGGCAACCGTTACTGCCGCCGTGTCTGTGAACCTTTACT
AGGCTACTACCCATACCCCTACTGCTACCAAGGAGGTCGAGTCATCTGTCGTGTCATCATGCCTTGCAAC
TGGTGGGTGGCCCGCATGCTTGGGAGAGTCTAATAGGAAGATTGAGTTCAAACGCTTAACCTTCTGTTAG
CCAATATATAATTAATGCATGCTACTCCATGAATTTCTGCCTATGAGGCATTTGCCTCCAAGTAGCCTAT
CCTTCAGAATTACTTGTAGGATATTCCTCTCTTCATGTTCTAATAAACTTCTACATCATCATCAAAAAAA
AAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:2811628 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2811628 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7404 same embryo
 EMAGE:7405 same embryo
 EMAGE:7400 same embryo
 EMAGE:7401 same embryo
 EMAGE:7402 same embryo
 EurExpress:euxassay_004572 same experiment
 MGI:4828854 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS