EMAGE mouse gene expression spatial database

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EMAGE:7404

Tmem174 transmembrane protein 174 ( MGI:1915594)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004426. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004426_01	euxassay_004426_02	euxassay_004426_03	euxassay_004426_04

euxassay_004426_05	euxassay_004426_06	euxassay_004426_07	euxassay_004426_08	euxassay_004426_10

euxassay_004426_11	euxassay_004426_12	euxassay_004426_13	euxassay_004426_14	euxassay_004426_15

euxassay_004426_16	euxassay_004426_17	euxassay_004426_18	euxassay_004426_19	euxassay_004426_20

euxassay_004426_21	euxassay_004426_22	euxassay_004426_23	euxassay_004426_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7404_wholemount_strong.wlz

7404_wholemount_moderate.wlz

7404_wholemount_weak.wlz

7404_wholemount_possible.wlz

7404_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7404_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7566
Entity Detected:	Tmem174, transmembrane protein 174 ( MGI:1915594)
Sequence:	sense strand is shown >T7566 CCCACGCGTCCGGACCCACGCGTCCGCGTGTGAGGAGCTATGGAGCACAGCAGTAATCGCCCAGAGGACT TCCCGCTTAACGTGTTCTCTGTCACTCCGTACACACCCAGTACCGCCGACATCCAGGTGTCCGACGACGA CAAGGCAGGGGCCACTTTGCTTTTCTCAGGCATCTTTCTAGGACTGGTGGGGATCACTTTCACTGTCATG GGCTGGATCAAATACCAAGGTGTCTCCCACTTTGAATGGACCCAGCTCCTCGGACCCATCCTTCTGTCGG TCGGAGTGACATTCATCCTGATCGCTGTGTGCAAATTCAAAATGCTATCCTGCCAGTTGTGCTCAGATAA CGAGGAGAGGGTCCCGGACTCGGACCAGACTTCCGGAGGACAGTCGTTCGTTTTCACTGGCATCAATCAG CCCATCACCTTCCACGGGGCCACCGTGGTGCAGTATATCCCTCCTCCTTACGGTTCTCAGGAGCCCCTGG GAATGAACGCCACCTACCTGCAACCCATGATGAATCCTTGCGGTCTCATACCTCCTAGTGGAGCAGCGGC TGCCGCACCAAGTCCCCCTCAGTACTACACCATCTACCCTCAAGACAATGCTGCGTTCGTGGAGAGTGAG GGCTTCTCTCCTTTCGTGGGCACTGGATATGACAGGCCCGACTCTGATGCTGACCAGCTAGAAGGGACGG AGTTGGAAGAGGAGGACTGCGTATGTTTCTCTCCTCCACCGTATGAGGAGATATACGCTCTACCTCGCTA GAGACTGCAATGCTAAGGGGACGGACATTTAAGCCCTGTGATGTGATACTTGGAGAGTTTATCGCTGTGT TCTTCAGAAGTTAGGTGTCAAAGCAGCTCAGGAGATCTTACAGATGTCATTCAAGGTGGGAAAGAAGTGC CCCGAGACTGCTAAATTAAGCTGCCCTGGTTAAATTCCCCTCTGCTCTGGTTTTGAATTCTCTCAGCTAA GAAACCCTCTGCAGCTGGAGAGTCGCTCTGTGATAGAGTGATTTTGGAGCCCACGCAGTGCCTTGGGTTT GATCTCTAGAGCCAGAAGAAAACAAAAACAAAAACAAAAACAAAAACAAAACAAGACCTCTCTACATAAA GTGCAGGAGGAAAATTCACCCATTTCCCCATCCCCCACCCGATATCCATTTGAAGGATATCTTAGTTTTG AAAGATTGTCTTAGTTTTAAATCCGGCAGCCATGGCAGCTCTCAGACTGATGAAAGGGAGGCTGGCAAGC AAGCAGGGAAGAGAGCAGGCTCAGGTAGAAATTTCCCTGCACGGCGCTGAACCTTCCGCAGCAGAGTGAC TTATCTTAGACAACTTGGGCTGTTATCTGGTCTCCCTGGAAGCCTTTGGATCTTGAAGAGTTTGTTAAAG AAATAAAATCCATTAAGAAATAAATGAATAAGTAGAGTGGGATGAAACAGTGCCCCATGTTAGAATAGTG TTGGGTGGCCGATCCTACTGTGGACGAGGTAACAGGAGGATAATGAATGTCACCATGTGCTGTCCACCGA GTTACAGTGACCCCTGCTCCTGATGGTTTTCCTTTGCAAGGCTGAAGTTCAAGGCGTAATGTACATGGGT GAGCGCCTGCTCCCTCTGCCCACCCCCAGGCTGTGATTCCCAGGCACGAACTAGCTCAGCCGAGTGGCTT ACAGAACGCAGGTACAGCTGAGTGGCTTATGGAACACAGGTATGCCTCTGATCTGTTCCACAGAGCCATG CTGCCGTGTCGCTTTGTAGTCATGAATCATGGAGATGATCAGTCATCCCGTCTCCCCCACCCCCCGCCCC CGGGCGTAGCTCTCACCTTCATTTGAACAAAGAAAAGCTGGTAGCCTTCAGCTTCCTAAGTCTGAACGGT GTCACCAACCACAGCCCAAAGCTGCAGACTTTAGGAGGTGTCCAAAGAATTAGAAAGAAAACAGTTTTAC AAAGATCAAAGGCCACTCAAGGTAAAGGTGGCTGCCCCCAAGAGAGATACAGGAATTGTCAGGTCTTGAA GGTTTTGGTACTGTGCTTATATGTGGGATTGCTTTTACTCTCTGTCAGAAGAGTCCAGGTTTTTCAAGGA TATCAGCAAAACAATCTTGGTTTATTATTGTGATTCATATTATGCCTCAGGGACATTTCACTTGGATGAT AACTAGTAATAAAAAACTAGACATGTCTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4241631 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4241631 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7405 same embryo
	EMAGE:7400 same embryo
	EMAGE:7401 same embryo
	EMAGE:7402 same embryo
	EMAGE:7403 same embryo
	EurExpress:euxassay_004426 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source