EMAGE mouse gene expression spatial database

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EMAGE:7406

Cdc25b cell division cycle 25 homolog B (S. pombe) ( MGI:99701)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004713. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004713_01	euxassay_004713_02	euxassay_004713_03	euxassay_004713_04

euxassay_004713_05	euxassay_004713_06	euxassay_004713_07	euxassay_004713_08	euxassay_004713_09

euxassay_004713_10	euxassay_004713_11	euxassay_004713_12	euxassay_004713_13	euxassay_004713_14

euxassay_004713_15	euxassay_004713_16	euxassay_004713_17	euxassay_004713_18	euxassay_004713_19

euxassay_004713_20	euxassay_004713_21	euxassay_004713_22	euxassay_004713_23	euxassay_004713_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7406_wholemount_strong.wlz

7406_wholemount_moderate.wlz

7406_wholemount_weak.wlz

7406_wholemount_possible.wlz

7406_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7406_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8686
Entity Detected:	Cdc25b, cell division cycle 25 homolog B (S. pombe) ( MGI:99701)
Sequence:	sense strand is shown >T8686 CTTCCCCTCACCCCAGGCTCACTCTCGGAGCTGAGCCAGCTGGGTCGGCGTCTGCTGGCCGCTGTACTGT GGCCCTCTAGCTAGCCTTTGCCCGCCCCGCCACGATGGAGGTACCCCTGCAGAAGTCTGCGCCGGGTTCA GCTCTCAGTCCTGCCCGCGTGCTGGGTGGCATTCAGCGGCCGCGCCACCTCTCGGTCTTTGAGTTTGAGT CTGATGGCTTCCTGGGGTCTCCGGAGCCTACAGCTTCCTCCTCTCCGGTTACCACTCTTACACAGACCAT GCACAACCTCGCTGGGCTCGGCAGTGAGCCTCCAAAAGCTCAAGTAGGAAGCCTGTCGTTCCAGAACAGG CTGGCAGACCTATCCCTGTCCAGGCGCACCTCTGAGTGCTCCCTGTCATCTGAGTCCTCAGAATCTTCGG ATGCAGGTCTGTGCATGGACTCCCCCAGCCCTGTGGACCCGCAGATGGCAGAGCGCACGTTTGAACAGGC CATTCAGGCAGCCAGTCGGGTCATTCAAAATGAGCAGTTTACCATAAAACGCTTCCGATCCTTACCAGTG AGGCTGCTGGAACACAGTCCGGTGCTGCAGAGCATCACCAACTCCCGAGCACTGGACAGCTGGAGGAAAA CTGAAGCAGGCTACCGAGCCGCCGCCAATAGTCCTGGGGAGGACAAAGAGAATGTGCGCTTCCAGAGACC AAGATGGAGGAGCTCCAGGGAGAGAAGGTGTCTTACCACTGAGTGGAAGATGGAAGTAGAGGAGCTGAGC CCGGTGGCACAGTCTTCTTCCTTGACTCCTGTCGAAAGGGCTTCTGAAGAAGATGACGGATTTGTGGACA TCCTGGAGAGTGATTTAAAGGATGACGAGAAGGTCCCCGCGGGCATGGAGAACCTCATTAGTGCCCCACT GGTCAAAAAGCTGGATAAGGAAGAGGAACAGGATCTCATCATGTTCAGCAAGTGCCAGAGGCTCTTCCGC TCCCCATCCATGCCATGCAGTGTGATCCGACCCATCCTCAAGAGGCTAGAGCGGCCCCAGGACCGGGATG TGCCTGTCCAGAGCAAGCGCAGGAAAAGTGTGACACCCCTGGAAGAGCAGCAGCTTGAAGAACCTAAGGC CCGTGTCTTTCGCTCAAAGTCGCTGTGTCATGAGATTGAGAACATCCTGGATAGTGACCACCGTGGACTG ATCGGAGATTACTCTAAGGCCTTCCTCCTGCAGACCGTGGATGGCAAACACCAAGACCTTAAGTACATCT CACCAGAAACTATGGTGGCCCTGTTAACAGGCAAGTTCAGCAACATCGTGGAGAAATTTGTCATTGTGGA CTGCAGATACCCCTATGAGTATGAAGGCGGGCATATCAAGAATGCTGTGAACCTGCCCCTGGAACGGGAT GCTGAGACCTTTCTGCTGCAGCGTCCCATCATGCCTTGTAGCCTGGACAAGAGAATCATCCTCATTTTCC ACTGTGAATTCTCGTCTGAGCGTGGACCACGAATGTGCCGCTTCATCAGGGAACGGGACCGTGCAGCTAA CGACTACCCCAGCCTGTACTACCCGGAGATGTACATCCTCAAAGGCGGCTACAAGGAGTTCTTCCCACAG CATCCGAACTTTTGTGAGCCCCAGGACTACCGACCCATGAACCACGAGGCTTTCAGGGATGAGCTGAGGA ACTTTCGCCTTAAGACTCGCAGCTGGGCTGGGGAACGGAGCAGGAGGGAACTTTGTAGCAGGCTGCAAGA CCAGTGATGATGAGCCTGCTGCCATCCTTACCTCGTGAGGCTTGGGAGCCAGTGGGTCCCATGGGCCTGT GAGGCCACCTACCTTATAGAGGCCTCAGGTGCTATAGGGGGTTGGGGGCATGGTGTGGTGTCACATCTGT CTGCCCCTGTCCTCAATTTTCCTGTCTCACTCCACTTATTTTCTGTATCTTGGTACTGGTCCCAGCTTAA AGAGCTGAACCTGAGGGTGGATGCCAGCTGAGGAGAAGTCTGTTGTGTTCAGTGGGAGTCCTTTTACAGT CTTTTTTCCTTGTTTGTGTTGACTCTTTGTCTTCCCGCTTTTTGGGGAGAGCCTCAGCCCGTTAGGATGG CACAGTAGCTTCTTGCATGGCCTGGATGCAGGATGCTACTCTGCTACACAGGGCTGTGTCTACTCTCCTC TCCTGGCCACTGCATAGACTTATGCTCTCCATTGGAAGCATAAGTGCTTTCTCTTCTGTTGTAGTCTTAC CTGTAGCTTTCATTTTTTTGTCTGTCTGACACACTGACCGCTGGGGAGGCTTCCTTGTACAGCTTGGGGC TAGAGATTCAAATATCACTTACTCGTTAGGCCTGGCTTTTGTTAGCCCAGAAAGGGATCTTGCCCTTTAA GACCCCCAGGGCTAGAGGGAAGGCCTGACTCAGGAGCCTCTGGGAGCCCCATCCTCACTACTGTGAACCC CAGAGCCTCGCTGGTCAGCACTTGCTGCTGGGCAATGGACCAGTCACCGAGCTTTGCTGTTTGATCCTTC TCTGCCCTTCCCTGTTTTCCTTTCTATATCTGGCCCAGAAGACCTCTTGTATGTGTGGTTTTTCTGTGTT GTACTAGTGACTTGAGTCTAGGCCCTTTGTTGCATGGTCATGGATGCACAGTGCCTTATATACATGTATG CACACAAACCGGGTCCAAGTATTTTGGTAAGCATGATGGCCTATGGCAGGAGTGTGTGTGTGCGCGTGTG AACAAAGTCATTACACTTAGTGTTTGGAAATGTTAAAGAAGCATTGTTATGGGGAGGGGGGAGCAACCTC TGGGTTCAGAATCTACATATGCTGGAAGGCCCCAATGAGTCCTCTGTTGGGGGCCTTGGTTCAATAAAGC ACTGAGCAAGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6401489 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6401489 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7411 same embryo
	EMAGE:7407 same embryo
	EMAGE:7410 same embryo
	EMAGE:7409 same embryo
	EMAGE:7408 same embryo
	EurExpress:euxassay_004713 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source