EMAGE mouse gene expression spatial database

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EMAGE:7410

Emilin3 elastin microfibril interfacer 3 ( MGI:2389142)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004718. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004718_01	euxassay_004718_02	euxassay_004718_03	euxassay_004718_04

euxassay_004718_05	euxassay_004718_06	euxassay_004718_07	euxassay_004718_08	euxassay_004718_09

euxassay_004718_10	euxassay_004718_11	euxassay_004718_12	euxassay_004718_13	euxassay_004718_14

euxassay_004718_15	euxassay_004718_16	euxassay_004718_17	euxassay_004718_18	euxassay_004718_19

euxassay_004718_20	euxassay_004718_21	euxassay_004718_22	euxassay_004718_23	euxassay_004718_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7410_wholemount_strong.wlz

7410_wholemount_moderate.wlz

7410_wholemount_weak.wlz

7410_wholemount_possible.wlz

7410_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7410_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8698
Entity Detected:	Emilin3, elastin microfibril interfacer 3 ( MGI:2389142)
Sequence:	sense strand is shown >T8698 GCCCCGGCCACTCGGCGGGACGCAGCGGCCGCACAGCTCCAGGCGGCCGAACCAGCGCGCTCCGGGGGTC GGTCGCGAGTGGCCGCGGGAGCCCGGAGGGCGAGCGGAGGATCCCCCGCGGGAGGCACCCCGAGGGACAG AGTGACGACCGAGCGCGATGGGCCGCCGTCTGTCCGTGTGGCTGTGCACCGTCGCGGCGCTGCTCTCGGG GGCGCAGGCCAAGGGCACCCCTCTCCTCGCGCGGCCCGCACAGCCCAGTGCCTCCCGTTACAGCCTCTAC ACCACCGGATGGCGTCCGCGGCTGCGCCCGGGGCCACACAAGTCTCTCTGTGCCTATGTGGTACACAGAA ATGTGACCTGCGTCCTACAAGAAGGAGCAGAGAGCTATATAAAGGCTGAGTACCGGAACTGTGGGTGGGG ACCCAACTGCCCTTCAACAGTCAGGTACCGCACAGTGTTCAGACCCAGATACAAGATTGGCTACAAGACC GTGACAGACCTGGCGTGGCGCTGCTGCCCTGGTCTTACTGGGGAAAGCTGCCCTGAACACCTCACAGACC ATGGAGCCACCCCACCCCACCAGGAGCCAGAGCCTCAGATTCCCTTGGGGCAGCTGGGCCCAGGTCCCAG GCCTTCTCCTTACAGCAGAGAGGCCCCGCGCCCCCGTGGAAGAAAAGGCCAAGGGCCGTTTGGTGAGCGG CTAGAACAGCGCCTGTCGCAAGCATATGGCACCCTGAGTGGATTGGTAGCCAGCCACGAGAATCCCAATA GGATCACTGGTGACTCCAGGGCCCCTGTCGTACCTATAGGCTTTGGGGTCATCCCCGAGGGTCTTGTGGC CCCAGAAGACAGAGGCAGAGGACCACTCATCCCTCCTCTGAGTGAGATCCTGAGCAAGGTCACAGAGGTG AGCAACACATTACAGACCAAGGTGCAGCTGTTAGATGAGGTGCGTGGGCTGGCCCTGGGGCATGAGGCTC ACCTGCAGCGGCTCCGGGAAGCCCCGCCATCCCCGCTGACCTCCTTAGCGCTCCTAGAGGAATACGTGGA CCAGCGGCTGCAGCGACTCTGGGGGAGCCTGCTGGATGGCTTTGAGCAGAAGCTGCAAGGTGTCCAGAGC GAGTGTGACCTTCGGGTGCAAGAGGTGCGGCAGCAATGTGAGGAGGGCCAGGCAGCCAGCCAGCGCTTGC ATCAGAGCCTCGATGGCCGAGAGCTTGCCTTGCGTCGGGAGCTCTCCCAGCTGGGCACTCAGCTGCAGGG CTTGACTCTGACTGGTGGGGGCACCTGCTGCAGCCAACTGGCTTTGATCAGTGCCCGGGTGGACAGCCTT GAGAGGAACCTGCAGGCTGTCACTGAGACCCAAGGTGGCCCGGGCACCCTGGCCGCGGATGAGCTTGCGC GCCTCTCTGCAGCTATGCTTCAGGGAGGTGTGGACGGGCTGCTTGAAGGCCTTGAGACTATCAATGGGAC AGAGAATGGAGCAAGGGGCTGCTGTCTGAGAATGGAGGTGGGGGGCTGGGGTGTCGGTGGCTTCGGGTCC ACGCTGGAACAGCGAGTGCAGAGCCTAGAGGAGCGGCTGGCAACTCTGACCGGAGAGCTAAGCCCCGAGA GTGCCATACCAGACAGATCAGCGCGGCCGCTTGTTCATTCAGAACTAGCAGTACTGGAACAAAGGCTGGT GTCCCTGGAGACCTCGTGCACCCCAAGTACCACCACAGCCATCCTGGACAACCTCGTGGCAGAGGTGAAG GCCTGGCAGAGCCGGAGCGAGGCTCTCCTACACCAGGTGGCTCGTCACACTGCCCTGCTCCAACAGCTCA ATGGCACGGTAGCTGAAGTCCAGGGGCAGCTGGCAGAAGGGACAGGCAGCTCACTGCAAGGGGAGATCAC TCTCTTGAAAGTCAACCTGAACTCAGTGAGCAAGTCACTCACGGGCCTCAGCGACTCTGTCAGCCAGTAC TCTGATGCCTTCTCGGCTGCCAACACATCCCTGGATGAACGAGAACGAAGAGTGGAAGCCGAGGTCCACA CCATCCAGGAACAGATCAGCAGCCAGGGCTCACGGCTTCAGGCTGGCCACAGGCAGGTCCTGAACCTGCG AGGGGAGCTGGAGCAACTCAAGGCTGGCATGGCCAATGTGGCTAGAGGGTTGAGCCGCTGCCGGGACACA GCCCAGGAACTCCAGCATACAGTGGGCCACTTTGACCAGAGGGTGGCCCAAGTGGAAGGAGCATGTGAGA GGTTGGGCCTGTTGGCAACCCACCTGAACAGTCTGCCTACTGAGCAGCTGAGGTCCAGGGAGGGCCTGTG GGGCCACATAGACAAGCTGAACCACACATTGGCCCAGCACACGCAGGATATCGCCCGGCTCCGGGATGAC CTGTTGGACTGCAGAGCTCAGCTGGCTGAGGTGAGGCCAGGGCGAGCTGACTAGGATCGATGCACAGAAA GCGAAAGTGCAGATCCCACCAACCCTGGGAATATCATCCCAGAGATCAACTCTACCTGGAAACACCTTCC AGCCCTCCCTGACTGTTTCAGCCATCAAAAGTCACCCTCAGAGCTGCAGCCTGGACAATTCAGGGTAACA GTCAAGGTGAGCTACACGGGATGCTGTCTCAAGAGTCAAGGCAAGGACGCCATGCCCGAACCCAAAGCTG GGCTTCTGAGATGGCACGCTGCATCGTCTGCGCGTCCTCTTATTTGCAGACACACTGGAGTTAGCAACTG TGCAGTTTCCTGACCAAGTCTCAGACAAGCTTGCCCACTGTTCCCTTTACTAACCAGGGACCAGTCACAG CACTCGGGGGGTGGGGAGGTGGGGCATCTCTTCCTTTGTCCCTGAGCTTCTAGCCTTAGATACTGGATGA GGTTTCTACAGGAAGATGATGCCCACTGGACCTCTCTGGCAGTTTCATAGGTGCTATTTCTGAACTAAAG ACCAGAGGCCACCTGAGGTTTCCAGCTCCCCACACATCCTTCAGAACAGAACAAAAGCCCAGCGGCCCAG CCAAGAGCGCTCCATATTCCTTTTGATCTTGCCTCGTGATGAGGGTGCAGGCAGCTGTAGAATCCCAAAG AAGGGATCCAAACAATGTTTGAAGACCCCACCCCAATTTATCTTTTTTCTTTGATTTCACAAATACACCC ACCCACACGGTCCTCTCTCCAGTTAGAGGAAAATCTCTCCCACCCACTATGGTCAGTACCGCTTGCCAAG GGCAGCCTTAGAGTATGCTGAGGCAGACCCCAGGAAAGGGGCTGGCACTGAATGAAAAGGAAGCTAAGCT TTTAAACGTCCACGCAAAACCCCAGCCTCGCAAGGAACTTCACAAACTGGAGAGAAGCTGGGGTGGAAGC CATTGAGGAAAACACATGTGTTTAGAGAGAGACTGACCATTTCTGCTGAGGCTCCTGGCCTCAGTTTCCC CCAATGCACCCTGGGCCTGCCGTGCTGTTTGTCTCTCCCACTGCCCTGCATTCAGTGTTTGCCCTCCACT CTGATGTGGATTGGAAAACAGGCTAAGGATTAAACCTTTTAACCGGTAAAAAAAAAAAAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6402350 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6402350 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7411 same embryo
	EMAGE:7407 same embryo
	EMAGE:7406 same embryo
	EMAGE:7409 same embryo
	EMAGE:7408 same embryo
	EurExpress:euxassay_004718 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source