EMAGE mouse gene expression spatial database

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EMAGE:7443

B3gnt2 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 ( MGI:1889505)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004519. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004519_01	euxassay_004519_02	euxassay_004519_03	euxassay_004519_04

euxassay_004519_05	euxassay_004519_06	euxassay_004519_07	euxassay_004519_08	euxassay_004519_09

euxassay_004519_10	euxassay_004519_11	euxassay_004519_12	euxassay_004519_13	euxassay_004519_14

euxassay_004519_15	euxassay_004519_16	euxassay_004519_17	euxassay_004519_18	euxassay_004519_19

euxassay_004519_20	euxassay_004519_21	euxassay_004519_22	euxassay_004519_23	euxassay_004519_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7443_wholemount_strong.wlz

7443_wholemount_moderate.wlz

7443_wholemount_weak.wlz

7443_wholemount_possible.wlz

7443_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7443_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5771
Entity Detected:	B3gnt2, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 ( MGI:1889505)
Sequence:	sense strand is shown >T5771 CTCATCGTGTCTTTGATGGCTTCATAACCTGAAGGTCCGAAGAGGCCAGATAGGCACCACCCTCAGGCCT TGGGCATCTATCTAGGTGGCTGCCGCTACTATGTACGCTGAGAGCAAAGGTATGAGAGATGAGTGTGGGG CGTCGAAGAGTCAAGTTGCTGGGCATCCTGATGATGGCAAATGTCTTCATTTATTTGATTGTGGAAGTCT CCAAAAACAGTAGCCAAGACAAAAATGGAAAGGGAGGAGTAATAATCCCGAAAGAGAAGTTCTGGAAGCC ACCCAGCACTCCCCGGGCATACTGGAACAGGGAACAGGAGAAGCTGAACAGGTGGTACAATCCCATCTTG AACAGGGTGGCCAATCAGACAGGGGAGCTAGCCACATCTCCAAACACAAGTCACCTGAGCTATTGTGAAC CAGACTCGACGGTCATGACAGCTGTGACAGATTTTAATAATCTGCCGGACAGATTTAAAGACTTTCTCTT GTATTTGAGATGCCGGAATTACTCGCTGCTTATAGATCAACCGAAGAAATGTGCAAAGAAGCCCTTCTTA CTATTGGCGATAAAGTCCCTCATTCCACATTTTGCCAGAAGGCAAGCAATTCGGGAGTCTTGGGGCCGAG AAACCAACGTAGGGAACCAGACAGTAGTGAGGGTCTTCCTGTTGGGCAAGACACCCCCAGAGGACAACCA CCCTGACCTTTCGGACATGCTTAAGTTTGAGAGTGACAAGCACCAGGACATCCTCATGTGGAACTATAGA GACACATTCTTCAACCTGTCCCTGAAGGAAGTGCTGTTTCTTAGGTGGGTGAGCACTTCCTGTCCAGACG CAGAGTTTGTCTTCAAGGGCGATGATGACGTGTTTGTGAACACCCATCACATCCTTAATTACTTGAATAG CTTATCCAAGAGCAAAGCCAAAGACTTGTTCATAGGTGACGTGATCCACAATGCTGGGCCTCACCGGGAT AAGAAACTGAAGTACTACATCCCAGAAGTCTTCTACACCGGCGTCTACCCACCGTATGCCGGGGGTGGTG GATTCCTGTACTCCGGCCCCCTTGCCTTGAGGCTGTACAGTGCGACTAGCCGGGTCCATCTCTACCCTAT TGATGATGTTTATACGGGAATGTGCCTTCAGAAACTGGGCCTTGTTCCAGAGAAGCACAAAGGCTTCAGG ACATTTGATATTGAAGAGAAAAATAAGAAAAATATTTGTTCCTATATAGACCTAATGTTAGTACATAGCA GAAAACCTCAAGAGATGATTGATATCTGGTCTCAGTTGCAAAGTCCTAATTTAAAATGCTGAAATAGACA TGAGCTGCATTTCACAGAAAGGCCTAGCCTGACTAGTTCCCATGGTGTGCTCTCACAATAGGTGAGTTCT GTGTGAGGCTATTAGCCTTCATGAGCAGGTAGCCCCTGGGCTCCCAAGCCCTCAGTCCTTCCCTTGCCTT GTGAAGAGGGAAGGCTGAAGACAGCTCAGCATGGCAGGGTGAGTGGTTATGACCCTTCCTCTGGCTGCCG GTCCTCAGTTTCTAATTTGTTTTCTTTCTCCTCCACAATTATGTATGTATGTGTGTATATATGTGTGTGT GTACATACATACATATATATATATGTAGGACACAACCTGGTGGCTTTGTGAAATGGAATTCTATGTATTT TCATAAGATTTTGAAAGTTGTCTAGAAAGTAGACTGATGTCAATCTCCCGTCACCCAGCAGTATTGTCCT TGTTACTAGAAACCGTTACTTCCTTTATGCAAGGAAAGCCACGCAGTGCGTGTAGTTCATCTTGTCAGGG CTTATGGCCATGAGGACAGAGGGGATTTTCTTTTTACTTGTGTTTGGTTTCCTGGGTGGCATCATGGTAG TTAACCTATTTTTAGTATTTGAAGATCATGAGTGTGATTCCCTAATGGCCAACTGGAGACTGAGTAGCCC GACAGCCATGGGTCTGTGAGTGTTCAGAGACTGGGAAGCATTCGCCACTTCTGAGCTTTGGACGTGATTA GTCAGTTAAAACCCCAAGATTCTATTCTTGCCATATTATCACGTATTCCTTAGATAAAATTCTGGGTAGT GACACTTCCCTGTCTCAGTGTAGAAGTGCCTGTGCTTTTATTTATTGTTCAGATCAAACACCAAAACATT TTCTTAAAAAATATTTTGTGTAATATTTTATTTGTATACAGTGTTTGTGAAATATTTAACTAGAGCATGA TATTTTATTTTTTCTCATTTTTAATTCTTTGAGAATTTCATACAATGTGTTTTGATTGTATTCACCTCCC CATCTCCTCCCAGATCCAGCATGATGTTTTAAATGTTAAGCTGTAACATGTTAGATAAAGTTAACTCTTA TTTTTGAATTTTAAAATTTGGATGGGGGGGTATGAACTGCTAGAGAAAATAAAGTTCTGCCAAAATATTG AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2654354 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2654354 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7447 same embryo
	EMAGE:7445 same embryo
	EMAGE:7446 same embryo
	EMAGE:7444 same embryo
	EurExpress:euxassay_004519 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source