EMAGE mouse gene expression spatial database

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EMAGE:7471

Tef thyrotroph embryonic factor ( MGI:98663)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005233. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005233_01	euxassay_005233_02	euxassay_005233_03	euxassay_005233_04

euxassay_005233_05	euxassay_005233_06	euxassay_005233_07	euxassay_005233_08	euxassay_005233_09

euxassay_005233_10	euxassay_005233_11	euxassay_005233_12	euxassay_005233_13	euxassay_005233_14

euxassay_005233_15	euxassay_005233_16	euxassay_005233_17	euxassay_005233_18	euxassay_005233_19

euxassay_005233_20	euxassay_005233_21	euxassay_005233_22	euxassay_005233_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7471_wholemount_strong.wlz

7471_wholemount_moderate.wlz

7471_wholemount_weak.wlz

7471_wholemount_possible.wlz

7471_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7471_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7588
Entity Detected:	Tef, thyrotroph embryonic factor ( MGI:98663)
Sequence:	sense strand is shown >T7588 GCCATGGACATGCCTGAGGTCCTCAAGTCCCTGCTGGAGCATTCTTTGCCTTGGTCAGAGAAGAAGGCAG ATAAGGAAAAGGGGAAGGAAAAGCTGGAGGAAGACGAGTCAGCAGCAGCCAGTACCATGGCCGTCTCTGC CTCCCTCATGCCACCCATCTGGGACAAGACCATCCCCTACGATGGCGAGTCCTTCCACCTCGAGTACATG GACCTGGATGAGTTCCTGCTGGAGAACGGCATCCCTGCCAGCCCCACCCACCTGGCACAGAACCTGCTGC TGCCTGTGGCAGAGCTTGAAGGAAAGGAGTCTGCCAGCTCTTCCACGGCATCGCCACCATCTTCCTCTAC TGCCATCTTTCAGCCCTCGGAAACCGTGTCCAGCACAGAATCGTCCTTGGAAAAGGAGAGGGAGACCCCA AGTCCCATCGACCCCAGCTGTGTGGAGGTCGATGTGAACTTCAACCCTGACCCTGCCGACCTGGTCCTCT CTAGTGTACCAGGGGGGGAGCTCTTCAACCCTCGGAAGCACAGGTTTGCAGAGGAGGACCTGAAGCCCCA GCCCATGATCAAAAAAGCCAAGAAAGTCTTTGTCCCTGATGAACAGAAGGATGAAAAGTATTGGACAAGA CGTAAGAAGAACAATGTGGCAGCTAAACGCTCCCGGGATGCCAGGCGCCTGAAGGAGAACCAGATCACCA TCCGGGCAGCCTTCCTGGAGAAGGAGAACACAGCCCTGCGGACGGAGGTTGCCGAGCTTCGCAAGGAGGT GGGCAAGTGCAAGACCATCGTGTCCAAGTACGAGACCAAGTACGGGCCCTTGTAACCTGTGCCCCTGCCC CGCAGGGCACTGCCTGCACCTCAGACCTCTGCCTGGGGCTTCCTGGACCCCACTCGTGTGGAGACTTAGG ACTCTTCATGGGCACATGGTGGCGTACCTGCTCTAGGAGCACTCACGTCTCAGCTTCATTATAACATGGC CAGCACATGTGATGACTTCCCCAGGGGACCAAGATCCTCTCCGATGGGGAACATGAGCGAATCTACGTAG ACGGGTAAATGGCCCAAGTCTCTTTTGTTGGATGCTCCAGCTGAATCAGAAGGGGCCTTCTGGAGTCCAG GTTCTCTTGTACAGAGTGCTCAGGCTTTCCTAGCCTTCCCCTCAGTCTCCAGTTTGGACTGACAAGGGCT GTCTACAGAATGAGTCATGATTATTGTCACCCTAATGTCGTCTCAGGTAGCAGGTGCATTTCCACTTGGG GTGTGTCTGTCATGTACCTCACCCTCCCCAAAATCTTTGAGAGAAACATTCATTCCCATCTGTCTCAGAG CCAGGACATGCTAATCCTGTTAGTATAGACAGGGTGGTGGACCACACAAGTGGGGTCTCTGGATAGCCTG GAATTAGGGTGGGGCTGGGGCTTGTGGTAGAAATGCACCCAACCATTCTTAGCTCCAACTTTTGTCTTGT GACTCTCAGGCCTGAAGTGGCCCTGGAATCAGTTTCCTCTGCATCCCACTTGCTATGGAAGAGAAATTTC CAGGAAGTCTGGTCTCTGGGGTCTAATTGCCAGCGCCTTTGTATCTCTGGGCCATTCTGAAGGCTCATGA CTTCCACTAGCTTGGTCTCTGATTATTTTCACCCAGTATCCCTTCTCAGTTCTTAGGTTTAAGAAGAAAA CCTATCACCTAGGAAGCTTGAGCTTCCAGGCCCCAGGGTACCACTTCTGCATGGTGTTTAGGAAACTGGC TTCCACAGTCTCCTGTTGTGGGCACCAGACCGAGGGGTTTTCTCTTGGGAAGCTCCATGGAACCCTTGCT CTGGCTCACCAATATACCCAGGCCAGAATCTAGTGTCCCTGAAGGCAGAAGAGGGCCTGCCACGTCACAG ATTAGAATCCCTCCCCATGGGTATCAGCAGGCCGCCGTATATGAGTTGTATTTGCTGAAGTTACTTACAG TGCAGCTCTCATGGGACAGCAGGCTCTGGAGACATTTACACTGGCCTGCAGTCCCTGTGGAGTCGGAGGG TGCCCACACAGCTCTCCCCGTGGGGCAGAGTGATTTCTTGCAGTTTTGGCTCCCTCACAGCTGCACTTTT CTCTTCTATTTGGCTGGATTGGACATCATGGAACCCTCTACTCATGTGGGTCATGAGGGCACCCTCCAGG AGGGTGTGTTTGTTTGCTCAGGGCGGGCAGCCTGTGTGAAGGAGACAGAGGTGATGAGGGTGCTTCATTA TACTTAACACTGGCTTTATTTTAAATGGTGGCTTAGTTTGGGGCCCCTATCTTTTAAGACTTTTCATAAT GATGTTAAGACCAGGGCAGACTGTCTACTGGCCATGGACTGCAGCCAGGAGATGCCCTGCCCCTCCTGGG ACCTGCACACCACCTCTCTGGGGAACTTGACAAAGGTCCCTAAGGCTAAGGGAGGTCTCCCTCCTACTAG GTCCCTGACTTTGACTCTGTGGTTCTCTAGGAACCGTGTGCACACTTGTCTCTGTTGTAACTACAAAGGG CAGTAGCATCCAGCATGTCAGTGTCTGCCCGGGCTGCCTGCCTCCTACCCACCCAGCACTATTCTCTGGG CTGGCTGTTCACCCAGAGGCAGTTCTCATTTTGTTTCTGGTGGACAAGCCTTGAGCTGAAAATCTCTTCG TTTTCTTTGACTAGAAAATGTAAGGTTTCTGTTTTTTAAGTTTTAGTTTTAAGGAATGGATACTTATCTC TGGATGCCATCTGTGTTCTGACCCTAGTGGGTGGGTGGGCCTTACCGTCTCCCCTTAGTCCCCGTTCTGC CTTGTGGTGCCCTCCTTTCCTTCCAGAAGTAGGGAGTGGGTTCTGGCTCCTCCTAAGTGCTTACAGAGTC ACTGTCTGCTTGCTTCTTGGAGAAGCCTCTGGAATGAAAAGCGGCAGGGAAACAAACCGGAAGGAGCACG CCTAGAGGTTGACATCTTGGAGAACAAAGTGTTCACATAAGCTTGGCTCCTCTGTTGGCTTTAGGACCCA AGGCTGCACAGAAAGAGTTGAGCTCTGCTCTTTATCTTGCTGTGGAAGTCTCCTGACTCCCTCTAGGAGC TGACAGCTGAAGCCTGAAGCTCTTGCACTTGGATCCTTGAATTCTAAGGAGTAACCAGGCTCCAGAAGCC AACTTCTATTTGGCTTTCCGTGGGTTTGTCTTAGGATGAGAAGTGTCATCACTTCCTAAACATCCTGTTC TCAGCTATGGCAGCCTCAGAAAAGACACCTGAATGAGTTAGAACCCTGCAGGAATGTACATTCAAGAGAT GTCTTGATAAAGAACTACGTGCTTTTGGAAAGAGCCACCCTTTGGAGGACCTGTTAGTCACCTTTATCTT CAGCCCAAAGGCTAAGTTATGTGGGTCTGACTCCTTTGGTTAGGGACAGCACTAGACAGTGACCCTAACC CTTGGTCCATACTCCCTGCTGTGATGGTGCTGTGGTCTGCTTTCCACTCAGGCAGCTCCTACAGTCCTCC AGCCTGGGGACAGATGGGAAGCTTTCCAGGGGCCCCCCGAGAGGTGTGCTCCCTGTAAGAGGGGCCTGTA AGAGGAGCTCTTGGTTTCCAGGGCAAAGCCCTACCCACAGCTTCTTTAACATCTGTTAATTAAGTGCAAT AAAATTTTCTAGAAAAAGGCAAAGATGATTTCCAGGTAGAGATTGCTGTCTTTTGGTGTTGGATGCCAGA ACACCACTTGGTTTTATTTTTCTAAGTGCATGTGATTGGTAGAGTGTGTGGGCTCTGCATCCTCCCTGGG AGCTGCATTCCAGCAGCCCCTCTCCCTACCTTTGCCGGGGGAAAGAGGCCAGGAGAAAAGCCCTTTGTCC CAGCTGCAGAGGTGGAAGCAGACAGCAGCCCTTTGGCCTTACATGTGTGTGTGTGCCTGTGCGAGTGTGT CCCTGCTGGTGGGCCGGAGTGATGTGTTATGGAGGGAAGTTGGGAATGTATCCTTTTCAAACAAAATTAA ATATTTTGAGACGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4242534 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4242534 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7473 same embryo
	EMAGE:7475 same embryo
	EMAGE:7474 same embryo
	EMAGE:7472 same embryo
	EurExpress:euxassay_005233 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source