EMAGE mouse gene expression spatial database

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EMAGE:7478

Fgf5 fibroblast growth factor 5 ( MGI:95519)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004614. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004614_01	euxassay_004614_02	euxassay_004614_03	euxassay_004614_04

euxassay_004614_05	euxassay_004614_06	euxassay_004614_07	euxassay_004614_08	euxassay_004614_09

euxassay_004614_10	euxassay_004614_11	euxassay_004614_12	euxassay_004614_13	euxassay_004614_14

euxassay_004614_15	euxassay_004614_16	euxassay_004614_17	euxassay_004614_18	euxassay_004614_19

euxassay_004614_20	euxassay_004614_21	euxassay_004614_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7478_wholemount_strong.wlz

7478_wholemount_moderate.wlz

7478_wholemount_weak.wlz

7478_wholemount_possible.wlz

7478_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7478_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7623
Entity Detected:	Fgf5, fibroblast growth factor 5 ( MGI:95519)
Sequence:	sense strand is shown >T7623 CTGGCGTTATAAATATCCCGGTGCCAGCGCCGAGATCCGCTCGGGTGGCCTCTCTCTCTCCCCCTCTCCC TCTCCCTTCCCCGAGGCTATGTCCACCCTGTGCGGCGAGGGAGGCAGCGCCAGAGGCACGCAGCCGCGCG GGGGCTACGGAGCCCGGAGCCAGCCCTGCAAGATGCACTTAGGACCCCCGCGGCCGGAAGAATGAGCCTG TCCTTGCTCTTCCTCATCTTCTGCAGCCACCTGATCCACAGCGCTTGGGCTCACGGGGAGAAGCGTCTCA CTCCCGAAGGGCAACCCGCGCCTCCTAGGAACCCGGGAGACTCCAGCGGCAGCCGGGGCAGAAGTAGCGC GACGTTTTCTTCGTCTTCTGCCTCCTCACCAGTCGCAGCTTCTCCGGGCAGCCAAGGAAGCGGCTCGGAA CATAGCAGTTTCCAGTGGAGCCCTTCGGGGCGCCGGACCGGCAGCCTGTACTGCAGAGTGGGCATCGGTT TCCATCTGCAGATCTACCCGGATGGCAAAGTCAATGGCTCCCACGAAGCCAGTGTGTTAAGTATTTTGGA AATATTTGCTGTGTCTCAGGGGATTGTAGGAATACGAGGAGTTTTCAGCAACAAATTTTTAGCGATGTCA AAAAAAGGAAAACTCCATGCAAGTGCCAAATTTACGGATGACTGTAAGTTCAGGGAGAGATTCCAAGAAA ACAGCTATAATACCTATGCGTCCGCGATCCACAGAACTGAAAAGACAGGCCGAGAGTGGTACGTGGCCCT GAACAAGAGAGGGAAAGCCAAGAGAGGCTGCAGCCCACGGGTCAAACCCCAACACGTCTCCACCCACTTC CTACCCAGGTTCAAGCAGTCCGAGCAACCGGAACTTTCCTTCACCGTCACTGTTCCAGAAAAGAAAAAGC CACCGGTGAAACCAAAGGTGCCCCTGTCGCAGCCTCGCAGAAGTCCCAGCCCAGTGAAGTACAGACTGAA GTTTCGCTTTGGATGATGCTCGTCCATCCTGGCCTTGTGGGAAACAACTCTCTATACAGGCGTCATCGGA GCCCTGAAGGAAACTCGGATACAGCATCCCTCTGCTGTGCCTACACGTGTGGAAGTCAGGTCATTTTGTT TCGCTTTGACTGGAACTAAACTGCTTTCTAAAATTTAAGTCGAAACAGAAGTGGGATTCTCTGTAGCGAT CCAGGGAGCTCATCTCTTCAGATCACCGAGGATATAAAGGCTTCGTGATTTGAGGATGGTTAGCATTCTG CGTTTTCAGACCAGTTTGATAGCCTGGAACTATGTACTTTTCTCATTTTTACAGATCAACCGGAAAGAAC ATACATGATACATGTTTATTTTTGTTTTCCAAAGAATATTTTGATGCAGATAAAATATTTTATAAACTTT TGTTTTTTTAAGGTACCTCAGCATTGAGCATATTTTCTTACTTTTATTATTTTAATTAACGTGACACAAG CAGTTGGTTTATTGCTGTTCATGGATTATAAACATTTTAATAGTTTGTGTGGTAGGATCTGGATCTCCTT TGCGTTTTTCTCTCTACTACAGCATCGTTTACTTTATTTTAGCTATACCTCAAGTCGTATAAATTTAGTT CTACATTTTAAAAAAAATTTTTAAATGTGCTTTCGAAGCCCCAAAAGGCTCAGCTTGGAGGTGTGCATTG TGTATGAAGTTATGGCATTATGTGGAATCTGGCCATATTAGTGGCTGGGCTCAATGATCAGAAGGAGGAA AATATGAGGACGTTTTGATGGATTTAAAGGCTTTATGTAAAGAAAAGGACAGAGGCCACCGCACACTAAG TGTGCAGCGTCCACAGAGATTTAACTCTGTCTTTTGCTTCTTCTCCTTTTATCTGCCCCCTGCCCTCATT CAAGACCAGCTGAAGGAATAAGTTTTATTCTGTCCACCAAGCATACACTAGGAAACTGGAGTGCTTAAAA ATACTTCTATAACTCCCTGTTATCCTTACTGTATGGACCCACAGGGAGTAACTAATCAGGGTGTGTGTGT GTGTGTGTGTGTGTGTGTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAGCTGAAGGAGATG AATCACTAACCAAGTTTTCATGTCAAGTCTCATGGTCAAAAATACTTCTACTATTCAGTCAAATACCCTT TGAGCTTTCTACCCTAAAGGTAATGACTGGAATGAGTGCATCTGCTCTGCTCTAAGAAAACCTGGTGCAC CCTAGAAGCTTCTGCCCTTGCGACCCAGGAGCTTAATTCGGGAATGTGATGAGCTGAAAATGACACTTCA TTCTCAACCCAAATTCCAAACCCTCAACAGAAACCACAGACGGTTTAGCTTACAAAGAGTTACAAAATGT ATTTTCTTGGATAATCCTTTTTATGGAGATCTATGAAGTATAAGTGTTCACCTTATATTCCTAAATGCTG CAATAAAGAGGCGTAGATGACCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:30443152 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30443152 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7481 same embryo
	EMAGE:7479 same embryo
	EMAGE:7480 same embryo
	EMAGE:7476 same embryo
	EMAGE:7477 same embryo
	EurExpress:euxassay_004614 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source