EMAGE mouse gene expression spatial database

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EMAGE:7500

Tacr3 tachykinin receptor 3 ( MGI:892968)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004597. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004597_01	euxassay_004597_02	euxassay_004597_03	euxassay_004597_04

euxassay_004597_05	euxassay_004597_06	euxassay_004597_07	euxassay_004597_08	euxassay_004597_09

euxassay_004597_10	euxassay_004597_11	euxassay_004597_12	euxassay_004597_13	euxassay_004597_14

euxassay_004597_15	euxassay_004597_16	euxassay_004597_17	euxassay_004597_18	euxassay_004597_19

euxassay_004597_20	euxassay_004597_21	euxassay_004597_22	euxassay_004597_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7500_wholemount_strong.wlz

7500_wholemount_moderate.wlz

7500_wholemount_weak.wlz

7500_wholemount_possible.wlz

7500_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7500_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal cortex

strong

regional

strong expression: see section 05 06 07 08 14 15 16

testis

strong

regional

strong expression: see section 04 05 06 07 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7605
Entity Detected:	Tacr3, tachykinin receptor 3 ( MGI:892968)
Sequence:	sense strand is shown >T7605 CCCGGCAGGACCCAAGCGAGGAGTCTGGTTCTTTCCTGCCCAGCAAACTCCCAGGAGTGAGGCTCCAGCT CTGCAGCCTTTGCCCCACAACCGCATCCCAGGGGGGCGGAACCATCCATCACTGCCGGCTACCAGGTAGT GCCCCAAAACAACGTGCCCAGGGAGTGAAGAACTTCAAGGGAGCGGGCTCCTGCTGGCCTCCGGAGTGTG ACAGGTCCCCAGCTGCAGACGCTGGCCATGGCCTCGGTTCCCACCGGCGAAAACTGGACGGACGGGACGG CGGGCGTGGGGTCCCATACAGGGAATCTGAGCGCTGCGCTGGGGATCACTGAGTGGCTTGCGCTCCAGGC TGGCAACTTCTCCTCCGCGCTGGGCTTGCCAGTGACATCCCAGGCACCTTCCCAAGTCCGGGCCAACCTG ACGAACCAGTTCGTGCAGCCCTCCTGGCGAATCGCGCTCTGGTCGCTGGCCTATGGCTTAGTGGTGGCGG TGGCAGTCTTCGGAAACCTCATCGTTATTTGGATCATCTTGGCCCACAAGCGCATGAGGACCGTCACCAA CTATTTCCTGGTAAACCTGGCTTTCTCCGACGCCTCCGTGGCTGCCTTCAACACCTTGGCCAATTTCATC TATGGTGTTCACAGCGAGTGGTACTTTGGCGCCAACTACTGCCGCTTCCAGAACTTCTTTCCCATCACAG CGGTGTTTGCCAGCATCTACTCTATGACAGCCATTGCAGTGGACAGGTATATGGCCATTATCGATCCTTT GAAACCCAGACTATCTGCCACAGCCACTAAGATTGTCATCGGAAGTATTTGGATTTTGGCATTTCTACTT GCCTTCCCTCAATGTCTTTATTCCAAAATAAAAGTCATGCCAGGCCGTACCCTTTGCTATGTGCAGTGGC CAGAAGGTCCCAAGCAACATTTCACGTACCACATCATTGTTATCATCCTGGTGTACTGTTTCCCATTGCT CATCATGGGTGTCACCTACACCATCGTTGGAATTACTCTCTGGGGAGGAGAGATCCCAGGAGACACCTGT GACAAGTACCATGAGCAGCTTAAGGCTAAACGAAAGGTTGTAAAAATGATGATTATTGTGGTGGTGACAT TTGCCATCTGCTGGCTACCCTACCATGTGTATTTCATTCTCACTGCGATCTACCAACAGTTAAACAGGTG GAAATACATCCAGCAGGTCTACCTGGCTAGCTTCTGGCTGGCCATGAGCTCAACCATGTACAACCCCATC ATCTACTGCTGTTTGAACAAAAGATTTCGTGCAGGCTTCAAGAGAGCATTTCGCTGGTGTCCTTTCATCC AAGTCTCCAGCTACGATGAGCTGGAGCTCAAGACCACCAGGTTTCATCCCACACGGCAGAGCAGCCTGTA CACCGTGAGCAGGATGGAGTCAGTGACCGTGCTGTATGACCCCAGTGAAGGGGACCCGGCCAAGTCCAGC CGCAAGAAGAGGGCGGTGCCCAGAGACCCCAGTGCCAATGGCTGCTCCCACAGAGAGTTCAAATCTGCCT CCACCACATCAAGTTTCATAAGCTCGCCCTATACCTCTGTGGATGAATATTCCTAAATCCATGTCCCAAG CTAAAGGCTGCCATGAGACCACCATGAGGCCAACCTAGGTCCCTCATTCTCCTAACCATGCCAGGTCCTT TTCTAGGTACTTTGGGAAAGCAGAAGGGCAGTCCTTAGGTGGGGATACTTCATTTGAGAAAAGTGGAATA CCAACATAACAAAAACTACCAACATATCGGCCAGCACAAAGAGAACTGAGTCTTCAATCATATGCAAAGT GTGAATCTGAAAGATGCTCTAAGTATCCGGTGTCTGTCATTTAACAATGGCAATGTGTCTTTGTGACCTC CGAACAGCAGAGGGGTTTTCCAAGAGAATAAGGAGGCTCTAAAATGCTATGATGAGCAAGGGAAACATGA CCTACATAAAACAGAAGTTCTGTGTGGCTGTGAAGTGGAGAGGGCAAATGACTGACTTCCCAAACAAGTA ATAGAAAACTCAGCCTCAAAGAGTAGGGAGATATGGTAGGAAGAAGGCTTTTCAAGAAAATTTATGTCAT AAAATAAAAACTTTCAGAAATTCCCTCCAAAACACTTAGCAGAAAAGGAGAGATAGTCAAAGCATTGCCC AAACACAGCATGTATTTCTCTTGATTATTTTTCCAAAGGAAATAATTTCATTTAATTTTGACCACTAGGT GTTTTGAGTGCTAAACTAAGTTGCAGTCAAATTTGGGGCTTTAAAAGACAAAGAAAATTCTTACCATCTT CTTTATCTTATTATTCATGCTTTTTAAAATTGAGTAAAATTGTAAAAACACTAAAGCTATCATCCAATGG AAAAAGAGAAATCCATATTTGCCTCGGTAACTCACTTGCTTGATTTGGATAACAAGTTAATAGTACTTAG ACGTCATCAAGCCAGACACCAGCATCTCCAAGTCCCTGAGCCAGCTGCTTCCCACTGCCATGCAAAGAAA ATCAAAGTCATGAACTTTACTGGTTATATCATGGCATCCTTCTCTTCTTTCCTCACTGAGAAAAAAACAA AGGCCATTGGGGGAGTATTACAATATATTTCAAGTGTGAGCTGAGTTGCCCCAGGACAAATTGAAAAGTA TTAATATATGTACTGATAAATCAGAGAAGACATAGGCCCATAAGTAGCTGGCTAGAAAAAGAAGATGTGT AGGACTCTGGGGACATAAGCTAGAGATGAGGAAGAGGACAGCACACATGCTCCCAGACACAAAACTTGGT TGTCTGTACTTAGTCAAGAGAAAATCCCTCTAGAAGGAAATGTGTTGGTATGTGTGTGTGTTTATGTATC TCTTCCTAAAGTTTGTTTGACTCATCTAATAGAAATTAGTATTTTTGGTAGTCTCTTTTCTTACCCCTAT AAATGCTTCCCTTGTTAATTTATGCTTGGAATACACTGTGAGATTACTTCCTTTCCAGTTAGGGGAACTG CCATGTGTAGGCACATTTAAGAGGCCCAGAACAAAATTACAGACATCTAGAAGTGTAGAATGCGTCTTCC AGACTACATCCTTTACAAGTCATCTTTCCTTATGTTACTACAACTGTTGATTCTGTTCATAAAATCGCAA GCCTTCTGGGAATGAGATTTGTCCTACAAACTTGTCTTTCCCAACATTCAAGTGGGTTTTATTAACTATC TGTCCAAAATACTTGCCTCTAACACGTTTTTCTTTGTATGCTCTATCAACATAAGAATATTTCTGAAGAT CAGGGCAGAGGCAATTGCCTGGTAGTAAATCATAGTGGATTATCACAGCATTTGTCTCTAGTCCTTTTTT CTCATTTGAAGCCCAAACCATATATACCAGACTGTTACACAATTTCAACTCTCTAAAGTACCAAATTATA TATGCTTGGGTTTACTTTAATAACTCCAATAAACAAACAGACAACCAAATACACATATGAGTGTGCAGAT AAACACTATCAAAAATGAATGTGATTGCTTCAAGGATTGAATTCAAAATTGAAACCAAAAAGGCAGATGG TCACAACAGGATTGCCCCAAAAGCCACTCAGTGATGTCTACGAAGCATTGTGTGCATGTTTCCCCTTAGC AATTGTATCTCATCATCTGAATATAGTGTTTGAAAAATGTTTATTCTTAAATTATGTAAGTGCTTATCAA ATGTAAAATCATCATGAAAACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30436441 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30436441 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7504 same embryo
	EMAGE:7502 same embryo
	EMAGE:7503 same embryo
	EMAGE:7501 same embryo
	EurExpress:euxassay_004597 same experiment
	MGI:4828583 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source