EMAGE mouse gene expression spatial database

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EMAGE:7502

Hapln1 hyaluronan and proteoglycan link protein 1 ( MGI:1337006)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004589. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004589_01	euxassay_004589_02	euxassay_004589_03	euxassay_004589_04

euxassay_004589_05	euxassay_004589_06	euxassay_004589_07	euxassay_004589_08	euxassay_004589_09

euxassay_004589_10	euxassay_004589_11	euxassay_004589_12	euxassay_004589_13	euxassay_004589_14

euxassay_004589_15	euxassay_004589_16	euxassay_004589_17	euxassay_004589_18	euxassay_004589_19

euxassay_004589_20	euxassay_004589_21	euxassay_004589_22	euxassay_004589_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7502_wholemount_strong.wlz

7502_wholemount_moderate.wlz

7502_wholemount_weak.wlz

7502_wholemount_possible.wlz

7502_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7502_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7600
Entity Detected:	Hapln1, hyaluronan and proteoglycan link protein 1 ( MGI:1337006)
Sequence:	sense strand is shown >T7600 CCACACCCACACCCACACCGCACTCGCCCAGAGACAAACTTAAGGTCAGGACGAGCACTAGCTCACTTCA TCTCCAGCTCCCAGCTAAGTGGGACTCAGCTTCTGAACTGGAGTTCAGGACAAGCAGAGAAGAGTCTGAG CTACAAGGATGAGAAGTCTGCTTCTCCTGGTGCTGATTTCAGTCTGTTGGGCTGACCACCACCTTTCAGA CAGCTACACTCCTCCAGATCAAGACAGAGTTATTCACATCCATGCAGAAAATGGCCCCCGTCTACTTGTG GAAGCAGAACAAGCCAAGGTCTTCTCTCACCGAGGTGGCAACGTGACACTGCCATGCAAATTTTATCGAG ACCCTACAGCATTTGGCTCAGGAATCCACAAAATCCGCATCAAGTGGACCAAGCTAACTTCAGATTACCT CAGGGAAGTAGATGTCTTCGTTTCCATGGGCTATCACAAGAAGACCTATGGAGGCTATCAAGGTCGAGTG TTTCTGAAGGGAGGCAGTGATAATGATGCCTCCCTGGTCATCACGGATCTTACCCTGGAGGATTATGGGA GATATAAATGTGAGGTGATTGAAGGGCTAGAAGATGATACTGCTGTGGTGGCATTGGAGTTACAAGGTGT GGTGTTCCCTTACTTTCCACGACTGGGACGCTACAATCTTAACTTTCACGAGGCACGCCAGGCTTGTCTG GACCAGGACGCAGTGATTGCTTCCTTTGACCAGCTGTACGATGCCTGGCGGGGTGGGCTGGACTGGTGCA ATGCTGGCTGGCTCAGTGATGGATCTGTGCAGTACCCAATCACCAAACCACGAGAGCCCTGCGGGGGCCA AAACACGGTGCCTGGAGTCAGGAACTACGGGTTTTGGGACAAGGATAAAAGCAGATATGACGTTTTCTGT TTTACATCCAACTTCAACGGCCGATTTTACTACCTGATCCACCCCACCAAACTCACCTACGATGAGGCGG TGCAAGCTTGTCTCAATGACGGTGCTCAGATCGCGAAAGTGGGCCAGATATTTGCTGCCTGGAAGCTTCT GGGCTATGACCGCTGCGATGCCGGCTGGCTAGCGGATGGCAGCGTCCGCTATCCTATTTCTCGACCAAGA AGGCGCTGCAGTCCGACTGAGGCTGCAGTGCGCTTTGTAGGTTTCCCAGATAAAAAGCATAAGCTATACG GGGTCTATTGCTTCAGAGCATACAACTGAGTGTGCCCTAGGAAGAGTCAGTTCTTGAGTTACGTGAAAGT TTTGTTTGTTTGTTTGTTTTTGTTGTTGTTGTTTTTAATATGAACTCATGCAAGTTACCAAAACTGTGAT AACCCTTTTTCACTTACTGTAAAGAGTCGTTTTCATAAAGGCCAATTCATTCATTTGTTTTGTAAAGCTA TCAGTCGATATATATATTATAAAGTAATGTAAGCGGAAGGCATTCTAAAATAGGGGGGTGGCGGGGGGTG CTCTAGAGCCTAAACTGGTTCATGCTACATCATCCCAACAAGATGCCATTTCATGAATGGGATATGTTGT AGGCTGAGAATCACCAGGATTGTAGTAAGGGAAGTAATGCTACTTGCCGCAGTAATTTCCACAAGCACAA ATTTTACATATTTCTACAATTTTGAAATGCACTTCGGTAAGCAAATTAGAACAACAAATTTGAAATACAG GCTTCTTTACATAAACCAGGATTCTGGGAGGTGCAAAAACTCACTATCATAAGGGAACAACAGAGACTTT TTCTAAAAGTTAATACTTTAAGTCTCCAGTATACAGAATAGTTTACTCTTTACAATTCTACCTTTTTGAC CAAAAAATTCATATAGACTCAGATGCAGAATAATAAAGCCACAGTTAGAACTCCTAGGTCAACTCATTTA TATGAAATATCTACTTAAGTACACAGATAATACTTTAGCAGTGAAAGAGTCCTACCTTCTAATTACAATT ACCTATCTGAGAAGACTTGTTTTATTATTTTTGAGTAGCTTTACTGAATCATATTTAAATTCTAAGAACC TTTGCTAAGCAGTATTTAGAATCTACTCAGGGCAGTTAAAACGACAAACTGCTGGACAGAAAATGTGTAA AATGTAGCAGCTTGATTTTATGTTAGCTTATGAAACATTATTGTCCTATAAAAATAATTTTAATCTATTT AATATTTCTTATTTACATTACATGACAAAAACCATCAAATGGATGAATAACTCTACACTTGCCTAAGAAA TAAAGACTCAACTTCCAAAGTTGTTCTTGTTTGTATCCACAATACAGTGCCCAAATAAGACCTTTATTTA TAGAAGAAAGGGAAAAATACATTTCTAAATAGTCTCCCAAGTAGGAAATACAGTCACTGTCAGTTTCCTT TCTGGTTAGAATTTGGCAAAAATAAAATACTGGCATTCTCATAGCAGACTTTACACCTCCAGGTTTTAAG TGAAAAGTTAAGCCCAGGTTCAGAAAAGTGACTTTCAGTATTAGCAACTCCAGTCTTAAATTAAAGTTTC TTGTTATGATTGCAGAGACCCCAGAGGGACCAATATTTATATTCAAGTAATATTTCTGGTTTCCCATTAT TTCACAGTGAATTCTTGTGAGTAGGCCTTAGCATAATAATCCAAGTACAACATGGCTAGTATGCTGTCAT CAATGTTTTTATGTAGATTTCCCCCCATGAACATAACATAAATAAATCTGTTTCCTGAATTGACCATGGT TGCATTTAAAGCTCTGTAATAACTATAATAAAAAATGCTCTATAAATATAGAGTTATGTGTGTGGAAAGT ATAGAGCAGATGGAAGTCCATGAAACCATAACTATCATGATATATTACCTAGATAAAATATTAGAAGTAA AAATAGAACCTTTCTTTCAGGTACTTAAGCCATGACAATGCATGAGGTTATAAGTTAGGATATGCAAATT ACACAAAAGTATACATCCAAAGAAAATGTGGGGTTTCACTCAAACAGTGCTGTTGTAAATTGAATTCAGA CTTCTAAGCTAAACACAAATTAAAGAGAATGAATGGTCTATAATATAATGACTGTATTACTTAAGGAGGA GACTCCAAACTAGCCCCTCAAACACCTTACAGGCTAGGCTAATCTCTTTGGAGGAAGATCATACTTGAAC AATCATTACGTATATTGTAAGAATTTTGTCTGCAATGCAAGTAGATAAATTTTATACCGAATTAGGTGCA CTCCATCCAGTTATTGTCATAGAACTCCACATTACAGCGCAGTAGCATTTCTTAACTTTTGAATACTTAG TATAGGAAATGGTGCTGATCAAAATCTCCAAAGTGACTGTTAGCTGACATATGTCCATACCAATTGTGCA CCATTTGTCCATTCTTGACAACATCCTAGAATGAACACTGCTGGGCAGAGTTTTTAACTTCTAATTTCAC ACAAAGGACCAGAATCGTCATGCCCTCCCTTTGAACTCCTAATTGAAAGGGGTCTTTTAATGTTCCCATC TTGCTGTTTTCACTCCACCAAATAATCTATTTTCCTACTTCATATCTCATTATAAATGTCTTCAAAAGAA AAAAAGTTGTCTTAGCACAATTCCTAGCAGAAAGTTGACATAACACTGTCTAATTCAGAAATCCCTTTCG AGACTTCAAGATTACCAGAGTATCAAGGTGATGAGAGGACAGACTTTAAAAAAATGAAAACATTTTATAG TATGGCCCACATATAAAGAACATAATAAATCTATAAATTTTACTGAAATGCCTTGATTTGAAACCTCTAA GCAAAAAGAACCAATATATTTAAAAATACATATAAGGATGAAGATATTTGTCATATTAACACTGGAAGCC ATCTTTTAATGACTGCTGCCATATTTTGTCACAGCAACTTTTAGCCAAATATAGTATTTGTAAATTTAAA TTTTCTTTCATATAAAGAGGGCCGGTACCTGTCAAAGTGCTATTTTATGCAGTAAGATGGTCGCTTGTTA TTTTGGCAATTAGTAGACTCAAATAAGATACCAATTTGGTTGCTGGCAGCCATACTGGATTTCTTGATTT CAGTACTCCTAAAAGCATGCTGGTGTCATGGAGTAGCTGACCACTAAAAAAGGGAGGATTCAAAGGGAAT CAGTTGTCAGGTTTGGTTTAGTCTTAGTTTTTTTTTTTTGTTGTTGTTTTGTTTTGCTTTGTTTTTTGCT TTTTTTTTTCCATTTAGTTTGTTTCATTTTGGTTTTGCATAGAATGCTTAGAGAAAACACTGACAACCTT TTTGAGAAGTATGTCATTGCTCAAGACTGCCAGCACAGTGTACAGCAAAAGCTATGAATAATACAGACAT CAGTAAGCCACCCAATTAGAAGAAGGTCAATATTTAAATGCATTTAATAGCTACTAAGTACAAGTTTGAG GAGAAACAATGTGTATTACAAAAAAGAATGTTTTCATTTTCTGTAAGATACAATGAACTGATGAACAAAT ATTAAAAGTCAATTTTGCTTTACTATAGTATCATTTGATTCTTATGACTTGTAGAATCAGTCATTTTTTC CCCTTGGGTCAAACTTGACAAATACACCATGCATTCTTCTGTTGCTAGCAATGCTTTGAATGAATACCCA CCCAAATGAGTTAGAGCTTTGACATTCAAACAAGAAAGTATACTTCAGAAATTTCCCTTAATTATTTGGA ATAAATTAAACAACCTTGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30430221 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30430221 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7504 same embryo
	EMAGE:7500 same embryo
	EMAGE:7503 same embryo
	EMAGE:7501 same embryo
	EurExpress:euxassay_004589 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source