EMAGE mouse gene expression spatial database

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EMAGE:7505

Sertad2 SERTA domain containing 2 ( MGI:1931026)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004566. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004566_01	euxassay_004566_02	euxassay_004566_03	euxassay_004566_04

euxassay_004566_05	euxassay_004566_06	euxassay_004566_07	euxassay_004566_08	euxassay_004566_09

euxassay_004566_10	euxassay_004566_11	euxassay_004566_12	euxassay_004566_13	euxassay_004566_14

euxassay_004566_15	euxassay_004566_16	euxassay_004566_17	euxassay_004566_18	euxassay_004566_19

euxassay_004566_20	euxassay_004566_21	euxassay_004566_22	euxassay_004566_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7505_wholemount_strong.wlz

7505_wholemount_moderate.wlz

7505_wholemount_weak.wlz

7505_wholemount_possible.wlz

7505_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7505_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7540
Entity Detected:	Sertad2, SERTA domain containing 2 ( MGI:1931026)
Sequence:	sense strand is shown >T7540 GCCCCCAGGCCGCCACGGCATGGGCTAGACCCCGAGCCGCTTCTTGCCGTCTCCCGTCCAGTCCGTGTGC GGTATCGCCCCTTTAGCCTGTCTCGCCCATCCCCGCCGTTTTTCGACAGTCAGTCCATCCCTCGGTCGGC TTTCCTCCGCGGGGCAGCGGTGGAGGACCAGCGGGCTCCGTAGGAGCGGGAGCCCCCACCCCGCTCCTCC TCCCGCTCCTCCGCGCTCTCACTCGTCGGGATCCCCGTCCCACACCTAGGGACGCGCCGACCGACGTCCT CTCCCCGCGGCCGTACGACCCGCCCGGGGGCTGGACCCGGCGCCCACTTCTTCGCGGAAGATATATGTTG GGTAAAGGAGGAAAACGGAAGTTTGATGAGCATGAAGATGGGCTGGAAGGCAAAATCGTGTCCCCCTCCG ACGGTCCATCCAGGGTGTCTTACACCTTACAGCGCCAGACTATCTTCAACATTTCCCTTATGAAACTGTA TAACCACAGGCCTCTGACAGAGCCCAGCTTGCAGAAGACTGTCTTAATTAACAACATGTTGAGGCGGATC CAGGAGGAACTCAAGCAGGAAGGCAGCCTGAGGCCTGCATTCACCCCCTCTTCCCAGCCGAGCAACTCAC TGAGCGACAGCTACCAGGAGGCCCCGCCTCCTGCTCCTCACCCCTGTGACCTCGGAAGCACTACACCCTT GGAGGCCTGCCTCACCCCTGCCTCCCTGCTCGAGGACGACAACGATGACACTTTTTGCACTTTGCAGTCT GTGCACCCTGCAGCTCCCACTAGACTCTCTTCTGCAGCCCTCCCAGCAGAGAAGGACAGCTTCTCCTCCG CCTTGGATGAGATCGAGGAGCTCTGTCCCACATCTACCTCCACAGAGGCTGCCCACACAGCAGCCCCTGA GGGCCCGAAAGGAACCTCCAGTGAGTCCAGTGTCCAGAAACCTGAGGGGCCCGAGGAAGGCCGCACAGAT GACTCACGATTCATGGACTCTCTGCCTGGGAATTTTGAAATAACAACGTCCACGGGTTTCCTGACAGACT TGACCCTGGATGACATCTTGTTTGCTGACATTGATACATCCATGTACGATTTTGACCCCTGCACATCTGC ATCGGGGACAGCCTCAAAGATGGCCCCTGTGTCAGCTGATGACCTCCTCAAGACCCTGGCTCCTTACAGC AATCAGCCTGTTGCCCCAAGTCAGCCTTTCAAAATGGATCTCACGGAGCTGGACCACATCATGGAGGTGC TTGTTGGCTCCTGAGACCTGGGCCCACGTGACTGCGCCCAGCCAGGCTCTGCACCCTTGCAGCCCCGACA GTTTCTCCGCACTGTGCATGATCCCTTGCTTGCCTTTTTCAGAGAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4240282 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4240282 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7509 same embryo
	EMAGE:7508 same embryo
	EMAGE:7510 same embryo
	EMAGE:7507 same embryo
	EMAGE:7506 same embryo
	EurExpress:euxassay_004566 same experiment
	MGI:4828003 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source