EMAGE mouse gene expression spatial database

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EMAGE:7512

Fah fumarylacetoacetate hydrolase ( MGI:95482)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004587. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004587_01	euxassay_004587_02	euxassay_004587_03	euxassay_004587_04

euxassay_004587_05	euxassay_004587_06	euxassay_004587_07	euxassay_004587_08	euxassay_004587_09

euxassay_004587_10	euxassay_004587_11	euxassay_004587_12	euxassay_004587_13	euxassay_004587_14

euxassay_004587_15	euxassay_004587_16	euxassay_004587_17	euxassay_004587_18	euxassay_004587_19

euxassay_004587_20	euxassay_004587_21	euxassay_004587_22	euxassay_004587_23	euxassay_004587_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7512_wholemount_strong.wlz

7512_wholemount_moderate.wlz

7512_wholemount_weak.wlz

7512_wholemount_possible.wlz

7512_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7512_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 10 15 16

facial vii ganglion

weak

regional

weak expression: see section 04

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 17 18

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 15 16 17

bladder

moderate

regional

Expression in the fundus region.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7594
Entity Detected:	Fah, fumarylacetoacetate hydrolase ( MGI:95482)
Sequence:	sense strand is shown >T7594 CAGCATGTCCTTTATTCCAGTGGCCGAGGACTCCGACTTTCCCATCCAAAACCTGCCCTATGGTGTTTTC TCCACTCAAAGCAACCCAAAGCCACGGATTGGTGTAGCCATCGGTGACCAGATCTTGGACCTGAGTGTCA TTAAACACCTCTTTACCGGACCTGCCCTTTCCAAACATCAACATGTCTTCGATGAGACAACTCTCAATAA CTTCATGGGTCTGGGTCAAGCTGCATGGAAGGAGGCAAGAGCATCCTTACAGAACTTACTGTCTGCCAGC CAAGCCCGGCTCAGAGATGACAAGGAGCTTCGGCAGCGTGCATTCACCTCCCAGGCTTCTGCGACAATGC ACCTTCCTGCTACCATAGGAGACTACACGGACTTCTACTCTTCTCGGCAGCATGCCACCAATGTTGGCAT TATGTTCAGAGGCAAGGAGAATGCGCTGTTGCCAAATTGGCTCCACTTACCTGTGGGATACCATGGCCGA GCTTCCTCCATTGTGGTATCTGGAACCCCGATTCGAAGACCCATGGGGCAGATGAGACCTGATAACTCAA AGCCTCCTGTGTATGGTGCCTGCAGACTCTTAGACATGGAGTTGGAAATGGCTTTCTTCGTAGGCCCTGG GAACAGATTCGGAGAGCCAATCCCCATTTCCAAAGCCCATGAACACATTTTCGGGATGGTCCTCATGAAC GACTGGAGCGCACGAGACATCCAGCAATGGGAGTACGTCCCACTTGGGCCATTCCTGGGGAAAAGCTTTG GAACCACAATCTCCCCGTGGGTGGTGCCTATGGATGCCCTCATGCCCTTTGTGGTGCCAAACCCAAAGCA GGACCCCAAGCCCTTGCCATATCTCTGCCACAGCCAGCCCTACACATTTGATATCAACCTGTCTGTCTCT TTGAAAGGAGAAGGAATGAGCCAGGCGGCTACCATCTGCAGGTCTAACTTTAAGCACATGTACTGGACCA TGCTGCAGCAACTCACACACCACTCTGTTAATGGATGCAACCTGAGACCTGGGGACCTCTTGGCTTCTGG AACCATCAGTGGATCAGACCCTGAAAGCTTTGGCTCCATGCTGGAACTGTCCTGGAAGGGAACAAAGGCC ATCGATGTGGGGCAGGGGCAGACCAGGACCTTCCTGCTGGACGGCGATGAAGTCATCATAACAGGTCACT GCCAGGGGGACGGCTACCGTGTTGGCTTTGGCCAGTGTGCTGGGAAAGTGCTGCCTGCCCTTTCACCAGC CTGAAGCTCCGGAAGTCACAAGACACACCCTTGCCTTATGAGGATCATGCTACCACTGCATCAGTCAGGA ATGAATAAAGCTACTTTGATTGTGGGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4242421 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4242421 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7516 same embryo
	EMAGE:7514 same embryo
	EMAGE:7515 same embryo
	EMAGE:7513 same embryo
	EMAGE:7511 same embryo
	EurExpress:euxassay_004587 same experiment
	MGI:4824681 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source