EMAGE mouse gene expression spatial database

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EMAGE:7513

Kcnj1 potassium inwardly-rectifying channel, subfamily J, member 1 ( MGI:1927248)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004592. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004592_01	euxassay_004592_02	euxassay_004592_03	euxassay_004592_04

euxassay_004592_05	euxassay_004592_06	euxassay_004592_07	euxassay_004592_08	euxassay_004592_09

euxassay_004592_10	euxassay_004592_11	euxassay_004592_12	euxassay_004592_13	euxassay_004592_14

euxassay_004592_15	euxassay_004592_16	euxassay_004592_17	euxassay_004592_18	euxassay_004592_19

euxassay_004592_20	euxassay_004592_21	euxassay_004592_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7513_wholemount_strong.wlz

7513_wholemount_moderate.wlz

7513_wholemount_weak.wlz

7513_wholemount_possible.wlz

7513_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7513_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7559
Entity Detected:	Kcnj1, potassium inwardly-rectifying channel, subfamily J, member 1 ( MGI:1927248)
Sequence:	sense strand is shown >T7559 TGTTGGTTTAAACCACACAACTCCACTCTTGAGTTAACCATTGAAAGCTAATGCAAGCAAGCATCATTGT GAGGCTTAAGATTCATTAAGGTGGGCCTCAAAGAAGTCGGCCTTTGCACAGACAAAACTGAACAGCACCA CTCACTTGCTTTGCCCAGCATGGATGCTTCAGATCGGAGGTGTATGTGCAGAGTGCTGATCAGGGCACTG ACAGAAAGGATGTTCAAACATCTTCGAAGATGGTTTGTCACTCACATATTTGGGCGTTCTCGGCAACGAG CAAGGTTGGTCTCCAAAGATGGAAGGTGTAACATCGAGTTTGGCAATGTAGATGCACAGTCGAGGTTTAT ATTCTTTGTGGATATCTGGACAACTGTACTTGACCTGAAATGGAGGTACAAAATGACCGTGTTCATCACA GCCTTCTTGGGGAGTTGGTTTCTCTTTGGTCTCCTGTGGTATGTCGTAGCCTATGTTCATAAGGATCTCC CAGAGTTCTACCCACCTGACAACCGTACTCCTTGTGTGGAGAACATTAATGGCATGACATCAGCCTTTCT GTTTTCTCTAGAGACCCAAGTGACCATAGGTTACGGATTCAGGTTTGTGACAGAACAGTGTGCCACTGCC ATTTTTCTGCTTATCTTCCAGTCTATTCTTGGAGTGATCATCAATTCTTTCATGTGTGGTGCCATATTAG CCAAGATCTCTAGACCCAAAAAACGTGCAAAGACCATTACATTCAGCAAGAATGCGGTGATCAGCAAACG TGGGGGGAAGCTCTGTCTCCTCATCCGAGTAGCAAATCTTAGGAAAAGCCTTCTGATTGGCAGTCACATA TATGGTAAGCTTCTGAAGACTACCATCACACCTGAAGGAGAGACCATTATTTTGGATCAGACCAATATAA ACTTTGTAGTTGATGCTGGCAATGAAAATTTGTTCTTCATTTCCCCACTGACAATCTACCACATTATTGA CCACAACAGCCCTTTCTTCCACATGGCGGCAGAAACTCTTTCCCAACAGGACTTCGAGTTGGTTGTCTTT TTAGATGGCACAGTAGAATCCACCAGTGCAACCTGCCAAGTCCGCACATCATACATCCCAGAAGAGGTGC TTTGGGGTTACCGTTTTGTTCCCATCGTATCCAAGACCAAGGAAGGGAAATACCGAGTGGATTTCCATAA CTTTGGTAAGACGGTGGAAGTGGAGACCCCTCATTGTGCCATGTGCCTCTATAATGAGAAAGATGCCAGG GCCAGGATGAAGAGAGGCTATGACAACCCTAACTTTGTCTTGTCAGAAGTTGATGAAACAGACGACACCC AAATGTAGCAGTGGCTTTTCTACCTACAAAGAGTCTCTCAAGGACCTAAGTGCTGGCTGTGTTCAGAAGC ATCCTGCAGTTGGGGGACTGAAAGCAGGATGAGAACCCAAGAAGTCTACCAGCAGAGTCACCCCCTCAGC CCCATGGCTATGATTCTAGAAGACTCGTAGCTCTAAAGTCTGCAGCAGTAAGCATACGGTGGAAGCATGT GAATGAACCTGGCGTGTGGAAGCCAAAAGGAGCTCACTTGGATCTTCACTCTGACTGTGTAAGCTCGTAC AGTGTTGATGGAAACAAAGTCATCCATTGACATGTACTGGAGAAAAGTTAGGAGCTTTAGAAAACTTCAG GAGCTAGCCATATTTCCTGTTTGATTCTATGGATGAGAAAGATATCACATTTTATCTTAAAGTAGACTTT TATCAACGGAAAATCTGTCTTCTGAGTTGGGAAGTGAGCCAACCAGTCACTGACAATAAGAGCCTGTCAT ACACAGAATCACTAAAGGCTCTAACTTCTTAAGCTCTGCTGTTTGAAGCCTTTGTCTGAGTCTGGGTCCA TGTTTCAGAAGGGGTAAGGTGGGCATCCAGTGACTGCACCTTTCTCAACCCAAGGTACAGAAGAACAGAA AGCCTCAACCAATTTCATAACTAACTCAGACGCTGCAGCCCATATAGGAATGGACCTGAATGATTTCCGG TGGTGCAATCAATGGAGGCCAAGCCCATTCCTTAGACTAAATGAACATTCTCTTGCAAAGGAGAGTGTTT GAGCTAAAAGTGTTGGTGAAGAGGCTGAAGAGATGACATTCTTTGTAAGTTCATGCTGCGCACGCATGAA GACCTGGGTCTGAACCTCCAACAATCACATACAAAGCTTGACTTAGTAGGATGCTCCCCCTCCCCAATCC TAGTACTGGGGAGGTAGGGATAGGAGGATGTCTCAAGTTTTCTGGACAGCTGAATGGGTGAGGTACCAGT TCAGGGAGAGACTACAAAAATAAGGTGAAGGGGCTGGCGGGAAGACTCTGGTTAAAAGCACCGTGGCTGA TCTTCCAGAGAACCTAGGTTTGGTTCCTGGCACAGTACTTCATAACTATCTATAACTCCAGTTTCAGGGC ATCTGATGCTCTTTTCTGGCCCCTATGAGCACCAGGTGCACATGCGAGCAAAACACTATACACATACGAT TAAAAAGATAAGGTAGAGAATGACTGAAGAAGAAATCTTACGTCAACTTCTGATCTACACACACACACAC ACACACACACACACACACACACAGAGCATAGTGATGTTTGTTTCAGTATTCAGTGGGTAGGTTATAGGGC CACAGGTCTGGTAAAAGAGGTTTTATTCCTTCCTCTCTCAAGATGGTGCTGAGGACTGGGAAAGCAGTCA GCCTGGAGTGTGTATGATGCTAACACCGAATGGTTCTTGTGTTTCTCTGACTAGTGGTTGAACTGCAATT CTAGGTATGAAGGAGAAAGGAACGAGATACAGCACCACAGTCAGGATGCTGCTGCTTTCTTGTCTCTAAA GGCCATCCCTACATCTTCCAAAGGGAACAAGTTAGCAGGAGAATTTACTTCGGAGGAAGGTTCTAATGTC TTGTTTGTTCATTTCCACATCTGCATCATTCTGATTTCACCTTGGGGGAAAAAAAGTAGAATTCAAAGCT GTTCAAAAATCAAAATATCATTTTAGTATGTGATTAATAAATTTCTGAAACTGTATGAGCAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4241262 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4241262 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7516 same embryo
	EMAGE:7514 same embryo
	EMAGE:7515 same embryo
	EMAGE:7512 same embryo
	EMAGE:7511 same embryo
	EurExpress:euxassay_004592 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source