EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7549

Hnf1a HNF1 homeobox A ( MGI:98504)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005232. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005232_01	euxassay_005232_02	euxassay_005232_03	euxassay_005232_04

euxassay_005232_05	euxassay_005232_06	euxassay_005232_07	euxassay_005232_08	euxassay_005232_09

euxassay_005232_10	euxassay_005232_11	euxassay_005232_12	euxassay_005232_13	euxassay_005232_14

euxassay_005232_15	euxassay_005232_16	euxassay_005232_17	euxassay_005232_18	euxassay_005232_19

euxassay_005232_20	euxassay_005232_21	euxassay_005232_22	euxassay_005232_23	euxassay_005232_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7549_wholemount_strong.wlz

7549_wholemount_moderate.wlz

7549_wholemount_weak.wlz

7549_wholemount_possible.wlz

7549_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7549_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

metanephros

weak

spotted

weak expression: see section 07 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7626
Entity Detected:	Hnf1a, HNF1 homeobox A ( MGI:98504)
Sequence:	sense strand is shown >T7626 AAGAGGTAATCCGGCAGGGGCCCTGATTCACTGGCCGCTGGGGCCAGGGTTGGGGGCTGGGGGTGCCCAC AGAGCTTGACTAGTGGGATTTGGGGGGGCAGTGGGTGCAGCGAGCCCGGTCCGTTGACTGCCAGCCTGCC GGCAGGTAGACACCGGCCGTGGGTGGGGGAGGCGGCTAGCTCAGTGGCCTTGGGCCGCGTGGCCTGGTGG CAGCGGAGCCATGGTTTCTAAGCTGAGCCAGCTGCAGACGGAGCTCCTGGCTGCCCTGCTCGAGTCTGGC CTGAGCAAAGAGGCCCTGATCCAGGCCTTGGGGGAGCCAGGGCCCTACCTGATGGTTGGAGAGGGTCCCC TGGACAAGGGGGAGTCCTGCGGTGGGAGTCGAGGGGACCTGACCGAGTTGCCTAATGGCCTTGGAGAAAC GCGTGGCTCTGAAGATGACACGGATGACGATGGGGAAGACTTCGCGCCACCCATTCTGAAAGAGCTGGAG AACCTCAGCCCAGAGGAGGCAGCCCACCAGAAAGCCGTGGTGGAGTCACTTCTTCAGGAGGACCCATGGC GCGTGGCGAAGATGGTCAAGTCGTACTTGCAGCAGCACAACATCCCCCAGCGGGAGGTGGTGGACACCAC GGGTCTCAACCAGTCCCACCTGTCACAGCACCTCAACAAGGGCACACCCATGAAGACACAGAAGCGGGCC GCTCTGTACACCTGGTACGTCCGCAAGCAGCGAGAGGTGGCTCAGCAATTCACCCACGCGGGGCAGGGCG GACTGATTGAAGAGCCCACAGGCGATGAGCTGCCAACTAAGAAGGGGCGTAGGAACCGGTTCAAGTGGGG CCCCGCATCCCAGCAGATCCTGTTCCAGGCCTACGAGAGGCAAAAAAGCCCCAGCAAGGAAGAGCGAGAG ACCTTGGTGGAGGAGTGTAATAGGGCGGAGTGCATCCAGAGGGGGGTGTCACCATCGCAGGCCCAGGGGC TAGGCTCCAACCTTGTCACGGAGGTGCGTGTCTACAACTGGTTTGCCAACCGGCGCAAGGAGGAAGCCTT CCGGCACAAGTTGGCCATGGACACCTATAACGGACCTCCACCGGGGCCAGGCCCGGGCCCTGCGCTGCCT GCTCACAGTTCCCCCGGCCTGCCCACAACCACCCTCTCTCCCAGTAAGGTCCACGGTGTACGGTACGGAC AGTCTGCAACCAGTGAGGCAGCCGAGGTGCCCTCCAGCAGCGGAGGTCCCTTAGTCACAGTGTCTGCGGC CTTACACCAGGTATCCCCCACAGGCCTGGAGCCCAGCAGCCTGCTGAGCACAGAGGCCAAGCTGGTCTCA GCCACGGGGGGTCCCCTGCCTCCCGTCAGCACCCTGACAGCACTGCACAGCTTGGAGCAGACATCTCCGG GTCTCAACCAGCAGCCGCAGAACCTTATCATGGCCTCGCTACCTGGGGTCATGACCATCGGGCCCGGGGA GCCTGCCTCCCTGGGACCCACGTTCACGAACACGGGCGCCTCCACCCTGGTTATCGGTCTGGCCTCCACT CAGGCACAGAGCGTGCCTGTCATCAACAGCATGGGGAGTAGCCTGACCACGCTGCAGCCGGTCCAGTTTT CCCAACCACTGCATCCCTCCTATCAGCAGCCTCTCATGCCCCCCGTACAGAGCCACGTGGCCCAGAGCCC CTTCATGGCAACCATGGCCCAGCTGCAGAGCCCCCACGCCTTATACAGCCACAAGCCTGAGGTGGCCCAG TACACGCACACCAGCCTGCTCCCGCAGACCATGTTGATCACAGACACCAACCTCAGCACCCTTGCCAGCC TCACACCCACCAAGCAGGTCTTCACCTCAGACACAGAGGCCTCCAGTGAGCCCGGGCTTCACGAGCCACC CTCTCCAGCCACCACCATCCACATCCCCAGCCAGGACCCGTCGAACATCCAGCACCTGCAGCCTGCTCAC CGGCTCAGCACCAGTCCCACAGTGTCCTCCAGCAGCCTGGTGTTGTATCAGAGTTCCGACTCCAACGGGC ACAGCCACCTGCTGCCATCCAACCATAGTGTCATCGAGACTTTTATCTCCACCCAGATGGCCTCCTCTTC CCAGTAACCGTGGTGACTGCCTCCCAGGAGCTGGGTCCCCAGGGCCTGCACTGCCTGCATAGGGGGTGAG GAGGGCCGCAGCCACACTGCCTGGAGGATATCTGAGCCTGCCATGCCACCTGACACAGGCTGCTGGCCTT CCCAGAAGTCTACGCATTCATTGACACTGCTGCTCCTCCATCATCAGGAAGGGATGGCTCTGAGGTGTCT CAGCCTGACAAGCGAGCCTCGAGGAGCTGGAGGACGGCCCAATCTGGGCAGTATTGTGGACCACCATCCC TGCTGTTTAGAATAGGAAATTTAATGCTTGGGACAGGAGTGGGGAAGCTCGTGGTGCCCGCACCCCCCCA GTCAGAGCCTGCAGGCCTTCAAGGATCTGTGCTGAGCTCTGAGGCCCTAGATCAACACAGCTGCCTGCTG CCTCCTGCACCTCCCCAGGCCATTCCACCCTGCACCAGAGACCCACGTGCCTGTTTGAGGATTACCCTCC CCACCACGGGGATTTCCTACCCAGCTGTTCTGCTAGGCTCGGGAGCTGAGGGGAAGCCACTCGGGGCTCT CCTAGGCTTTCCCCTACCAAGCCATCCCTTCTCCCAGCCCCAGGACTGCACTTGCAGGCCATCTGTTCCC TTGGATGTGTCTTCTGATGCCAGCCTGGCAACTTGCATCCACTAGAAAGGCCATTTCAGGGCTCGGGTTG TCATCCCTGTTCCTTAGGACCTGCAACTCATGCCAAGACCACACCATGGACAATCCACTCCTCTGCCTGT AGGCCCCTGACAACTTCCTTCCTGCTATGAGGGAGACCTGCAGAACTCAGAAGTCAAGGCCTGGGCAGTG TCTAGTGGAGAGGGTACCAAGACCAGCAGAGAGAAGCCACCTAAGTGGCCTGGGGGCTAGCAGCCATTCT GAGAAATCCTGGGTCCCGAGCAGCCCAGGGAAACACAGCACACATGACTGTCTCCTCGGGCCTACTGCAG GGAACCTGGCCTTCAGCCAGCTCCTTTGTCATCCTGGACTGTAGCCTACGGCCAACCATAAGTGAGCCTG TATGTTTATTTAACTTTTAGTAAAGTCAGTAAAAAGCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30471380 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30471380 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7547 same embryo
	EMAGE:7548 same embryo
	EMAGE:7551 same embryo
	EMAGE:7550 same embryo
	EurExpress:euxassay_005232 same experiment
	MGI:4825400 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source