EMAGE mouse gene expression spatial database

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EMAGE:7576

Cxcr6 chemokine (C-X-C motif) receptor 6 ( MGI:1934582)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005261. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005261_01	euxassay_005261_02	euxassay_005261_03	euxassay_005261_04

euxassay_005261_05	euxassay_005261_06	euxassay_005261_07	euxassay_005261_08	euxassay_005261_09

euxassay_005261_10	euxassay_005261_11	euxassay_005261_12	euxassay_005261_13	euxassay_005261_14

euxassay_005261_15	euxassay_005261_16	euxassay_005261_17	euxassay_005261_18	euxassay_005261_19

euxassay_005261_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7576_wholemount_strong.wlz

7576_wholemount_moderate.wlz

7576_wholemount_weak.wlz

7576_wholemount_possible.wlz

7576_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7576_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7682
Entity Detected:	Cxcr6, chemokine (C-X-C motif) receptor 6 ( MGI:1934582)
Sequence:	sense strand is shown >T7682 AAGAGGTAATCCAGTGAGCACACTTCACTCTGGAACAAAGCTACTGGGCTTCTCTTCTGGTTCTGACTCA CAGATGTTTGCCCCAACAGATGCCATGGATGATGGGCATCAAGAGTCAGCTCTGTACGATGGGCACTACG AGGGAGATTTCTGGCTCTTCAACAATTCCAGTGATAACAGCCAGGAGAACAAACGCTTCCTAAAGTTCAA GGAGGTCTTTTTGCCCTGTGTGTACCTGGTAGTGTTTGTCTTTGGACTGCTAGGAAACTCCCTGGTTCTG ATTATATACATTTTCTACCAGAAGCTGAGGACTCTGACAGATGTGTTTCTGCTGAACTTGCCCCTGGCTG ACCTGGTGTTTGTCTGTACTCTGCCCTTTTGGGCCTATGCAGGCACCTATGAGTGGGTCTTCGGCACAGT CATGTGCAAAACTCTTCGAGGCATGTATGCAATGAACTTCTACGTGTCCATGCTCACTCTCACCTGCATC ACAGTGGATCGTTTCATTGTAGTGGTCCAGGCTACCAAGGCCTTCAACCGGCAGGCTAAGTGGAAGATCT GGGGCCAAGTCATTTGCTTGCTCATTTGGGTGGTCTCCCTGTTGGTTTCTTTGCCACAGATCATCTATGG CCATGTTCAAGATATTGACAAGCTTATCTGTCAGTACCACAGTGAGGAGATATCCACTATGGTTCTTGTT ATACAGATGACTCTGGGGTTCTTCCTGCCATTGCTCACTATGATTCTGTGCTACTCAGGCATTATCAAGA CCTTGCTTCATGCTCGAAACTTCCAGAAGCACAAATCTCTAAAGATCATCTTCCTTGTAGTGGCTGTGTT CCTGCTGACCCAGACACCCTTCAACCTTGCCATGTTAATCCAAAGTACAAGCTGGGAGTACTATACCATA ACCAGCTTTAAGTATGCCATCGTAGTGACAGAGGCTATAGCATACTTTCGGGCTTGCCTTAACCCTGTAC TTTATGCCTTTGTTGGCTTAAAGTTCTGGAAGAACGTCTGGAAACTTATGAAGGACATCGGCTGCCTCTC TCACCAGGGAGTCCCAAGTCAATGGAAGTCTTCTGAGGACAGTTCCAAGACTTGTTCTGCCTCCCACAAT GTAGAGACCACCAGTATGTTCCAATTGTAGTAGGCCTTGCCACACTTAGAGAAGTTAATAACAGAATTCT AGGAGCATGGCTGTATCATTTGGATGCAACAAGAAAAGCTTTGCTTATAGCATGTGGAGTATCATGGAAA AGTCACTGAACACCATGGCTGGTACACAAAACTTCTCAGATATAAATATACCCTATTCTTAATATCTAAG CCTAATGCTCAAAGGAGAATGAGTATCTTGAGATTTTGAAGCACTTTCTCTCTTTCATCCTCAAGAATGC TGAAATCAAGGTCATGACGGTTAACTCTAGAGATCTCAAGTTTTCTTTTTACCAAGTTTGGGCCTAAAAG TTGAAGAGCTGCATCAACAAAGATGTTGCTGGCAGATAATGTTCAGGGCTCTTGAGTTCAGAAGATTCTT CAGTGGGCAAGCAGTTGAGAATTGAAAAGGCTCCATACAAATGCTGCTTGAATGAGACCAGGTTCTGACT CATCCAGGGGTCTGTCTGACTTTTGTATGGGTGGATGCTTATGTTTTACTGGAATTGTCTTGTAAAGTGC AGCAATATGGGACTGAAAACAGGCCAGAATAAATAAGCTGAATTTCAGTGGTCCATGTAATGCTAGAAAA ATGTATAAAACAGAGCTTAAGACTTTAACAAATACAAGAGGACTCTGGTGGCTTTGCTCATCTAAAAAAA AAAAAATGAAAGAAATTGTGACACATACTATGTACACACAAATATATCAAGCATTTTGAGTTTCATGGCT AAGAAATAAAACCTTTTAAATGTTAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30748222 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30748222 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7574 same embryo
	EMAGE:7573 same embryo
	EMAGE:7575 same embryo
	EMAGE:7578 same embryo
	EMAGE:7577 same embryo
	EMAGE:7572 same embryo
	EurExpress:euxassay_005261 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source