EMAGE mouse gene expression spatial database

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EMAGE:7586

Arih2 ariadne homolog 2 (Drosophila) ( MGI:1344361)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004686. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004686_01	euxassay_004686_02	euxassay_004686_03	euxassay_004686_04

euxassay_004686_05	euxassay_004686_06	euxassay_004686_07	euxassay_004686_08	euxassay_004686_09

euxassay_004686_10	euxassay_004686_11	euxassay_004686_12	euxassay_004686_13	euxassay_004686_14

euxassay_004686_15	euxassay_004686_16	euxassay_004686_17	euxassay_004686_18	euxassay_004686_19

euxassay_004686_20	euxassay_004686_21	euxassay_004686_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7586_wholemount_strong.wlz

7586_wholemount_moderate.wlz

7586_wholemount_weak.wlz

7586_wholemount_possible.wlz

7586_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7586_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8659
Entity Detected:	Arih2, ariadne homolog 2 (Drosophila) ( MGI:1344361)
Sequence:	sense strand is shown >T8659 GCGGGGGTCGGCTCTGCCGGAGGAAGCTGCGCGGGCCTGAGCCGCGTCCGCCGGCTGCCTCCGCTGCCGC TACGCCTCCGTCAGCCTGGTTTGGTCTGGCTTGACCTGGTCCGGCCGGCCTGGGCTCTGCGGCCGCGAGG TCGCGGCTCCCGATGGAAATCCTATGACAGACAGTTTGGGTGATATCTGCAGGAAAGATCTTTAAACATT ACAGAAGACAATACACGCTAATGCATTTGAGAAAACGATAAAAATTGGGGGTAGGGAAAAAAGCAACTGT TTCTGCAACTTTGCTGGATGTTAAGATGTCTGTGGACATGAACAGCCAAGGGTCAGATAGCAATGAAGAG GACTATGACCCAAACTGTGAGGAAGAGGAAGAAGAGGAAGAGGACCCTGGGGACATAGAGGACTACTATG TGGGAGTAGCCAGTGATGTGGAGCAGCAGGGGGCTGATGCTTTTGATCCTGAAGAGTATCAGTTCACTTG CTTGACCTATAAGGAGTCTGAGGGCGCCCTCCATGAACATATGACCAGCTTAGCCTCTGTCTTAAAGGTA TCTCATTCAGTTGCAAAACTTATATTAGTTAATTTCCACTGGCAAGTCTCAGAGATTCTGGACAGGTACA GGTCTAATTCTGCTCAGCTGCTTGTTGAGGCTCGAGTTCAACCTAATCCATCCAAACATGTCCCCACAGC CCATCCCCCTCACCACTGTGCAGTGTGTATGCAATTTGTGCGGAAGGAAAACCTACTCTCTCTGGCCTGC CAACATCAGTTTTGCCGAAGCTGTTGGGAACAGCATTGCTCAGTGCTTGTCAAAGATGGTGTTGGTGTAG GAATCTCATGTATGGCTCAGGATTGTCCACTTCGAACACCAGAGGACTTTGTATTTCCCTTGCTTCCCAA TGAAGAATTGAGAGATAAATACAGGCGCTACCTCTTTAGGGACTATGTGGAGAGCCATTTCCAGCTCCAG CTGTGCCCCGGTGCAGACTGCCCCATGGTTATCCGGGTACAGGAGCCTAGAGCCCGCCGAGTGCAGTGCA ATCGGTGCAGCGAGGTCTTCTGTTTCAAGTGTCGACAGATGTATCACGCCCCCACAGACTGCGCCACAAT CCGGAAATGGCTCACCAAGTGTGCAGACGACTCCGAAACGGCTAACTACATTAGTGCTCACACTAAAGAC TGTCCCAAGTGCAACATCTGCATCGAGAAGAATGGAGGCTGTAATCACATGCAATGCTCCAAGTGTAAGC ACGACTTCTGCTGGATGTGTCTAGGAGACTGGAAGACGCATGGTAGTGAGTACTATGAATGTAGTCGGTA CAAGGAGAATCCTGACATTGTCAACCAGAGCCAGCAAGCACAGGCCAGGGAGGCCCTCAAGAAGTACTTG TTCTACTTTGAGAGGTGGGAAAATCACAACAAAAGCTTGCAGCTGGAGGCACAAACATATGAGCGGATTC ACGAGAAGATTCAGGAGAGGGTTATGAACAACCTGGGAACTTGGATTGACTGGCAGTACCTACAGAATGC TGCCAAGCTCTTAGCCAAGTGTCGATATACCCTGCAGTACACCTATCCATATGCATACTACATGGAATCC GGTCCCAGGAAGAAGCTGTTTGAATACCAGCAGGCTCAGCTGGAGGCTGAGATCGAAAACCTTTCATGGA AAGTTGAGCGTGCAGACAGCTACGACAGAGGGGACTTAGAAAACCAGATGCACATAGCAGAGCAGCGGAG GAGAACCCTGCTAAAGGATTTTCACGACACCTAAACTGGGCTGTGGCTGTGCCGGGGTGAGGAAGATGTA GCTGTGAGGCCGCCCGGCTGCCCTCCTGCAGGCTGTAGGCTCTGCCTTTCACCACCCAGGCAACAGCCAG GGCCCCACTCCTGAAAGACACTGGCAACACACCTCTTAGCCGATTTGTTTTCTCCTCATCTTTTTGATTT TTATTTCTACCAGGGTAGAGGCCGCGTTGAGCTGGCCTCTAATGAGGACTTTACATTTCCCCTGGATGGT TGTTGGGCGGGAGGGAACTTTTTTTTCCTGAATGGCTATTAATAGTATTAGATCATTACAACTTATGTAA TTTTCAAAGGTTGTACGATTATAAAGGAAAGAGGCAAAGCATAGATAACACAGAGCCCGATTAAAATAAA TTGGCATTGGAGTGCTTCACCCTTTAGCTATTTGACTTAGAATGTAACTTGATACTGCCCAGCCACTGAG CCTCAGCGTGTCTGCACTGCCGGGGGCCCTGGGACGGCCCCTGCTTTCCAGCATCATGTTTGACTGGAGC CGGAGTCCTGGGGAAGGTGCACGGGTGGCAGAGAGTAGGAAACTGAGTTGGCCTGCATAATAGGATGGAG CACACAGTTACTTACCTGGCCTTAGCTGGACAGCCAGGGCACAGAGCAGAGCAAGGTCGCACTGCAGGCT TGCTTTTAGTCTTGAATGTGAGGTTTTTCGCTAAGTCAGGCCATCCAGACCAGCACCACAGCAGAGGTGG GCTACCACACCTCTGGACTTGGAGACTGGTTCAGCATGGTGCTTCCTTTTGTTTCTTAAAGAAAAGAAAT CCACCTTTGAGAGGAGTTGTTCCCCAGATGAGTTTCTTTTTCCTTGGTTGTTCCTTTTGATCCAGTATTC AAGTGGTGTACTCTGACCTGGGTCACTGTCTTGTGTTCTTATGAACAGAGTTCATGAGGTCAGAGTTCAG CATGGCTCTTAGACCAAGGGCATGGGTCTTGTGGCTCAAGAGCTAGGTTTGCACAAGTTCTTTTTCTGTT CACCTAGAGTGTGGCCAGAAGCTGCTCCAGGGTGGGGTGTGTTTCTCTTTGCCTAGTTGCAGCTGCCAGA AGTCCCCATCTCTATGGTCTGGGAGTCTAAGGCCAGGAGAATCCCTGGTTTGTGGTCTCAGTTCCTGTAC TAGAAGGGGCTGGCCAGAAGAAAGGACCAGTTCTTGCACAGTCAAGTGCAAGGGAACAGGCAGCTTGCCT GGTGTCCTTCCTATCTTGAACCTGGTCCTGTGGGCCTTTGAAAAGTTAGATCTGTGATCTCTGGGGTTTC CATGGCTTTGTTCAGTGCCTCCCCTCTGAGACTGGCAGAGCTGTCTATACTTGACAGGACCTCCTTGAAC TCCTAGGAGAGCCTATAGAGAAATCTGTGAATATTATGATGCAGATTCTTTGATATTCATACATTTTCAC CTCCAGTCAGCAATTTCCTAGGTACCATGTAAAGCTGTAAAATTGAATTATTCTTGAAGCCAGGTGACAG CTGTGACGCCTGGGGTACATGGAGCTGCACGTCCTCTCCCACCTCCTCAGGTGTTTTGTCTCTTCATGTC ATCCCTGACAAGTGAGGTTGGGAGGTGGATTGGGAGTAATAGTATAGTAGAGTATGGTGAGTGAACCCTA ACACCAATGGGAGCTGATTGTGACCCAGGAGTGTCCCTGCTGCAGGCCCAGGGTGGCTGTGATGCTCTGT TAGCACACAGACCTTTCCATCAAGTCCTGCCAGTGGCACTGCCGTGACACAGCACCAGAGTTGCAGCTCT GCAGCCTAACTCCCCCTTGGTACTTTCTGAACTGCACAGCTGCCATCCTCCACTCCTCAGCTGTGACCCT GACCATTGGCACTGCAGGAGGCTCTGCCAACCTGTAGCTGGTGGCGTCTCATCTGTGGAAACCTTTGTTT TTGCACCCTCAGTGGTTGTCCTTGTTAACATCAGAACATCTGAAAGGTCAGGCAGAGGTAGGGTTTGGCT TGGTGTTTTTACCCTGCAAAGGGTAGAGAAAGGTGTTTCCATGACCTGACCAGATTCTTGTCTTTCTTCA AAGAAAAGTTGAGGTAATGTCATCTCGAAAAGTTGAGTGATTTATTCAAAATAAACTCATTTTCTGATTA TAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6400287 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6400287 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7588 same embryo
	EMAGE:7587 same embryo
	EurExpress:euxassay_004686 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source