EMAGE mouse gene expression spatial database

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EMAGE:7662

Ywhaz tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide ( MGI:109484)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005318. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005318_01	euxassay_005318_02	euxassay_005318_03	euxassay_005318_04

euxassay_005318_05	euxassay_005318_06	euxassay_005318_07	euxassay_005318_08	euxassay_005318_09

euxassay_005318_10	euxassay_005318_11	euxassay_005318_12	euxassay_005318_13	euxassay_005318_14

euxassay_005318_15	euxassay_005318_16	euxassay_005318_17	euxassay_005318_18	euxassay_005318_19

euxassay_005318_20	euxassay_005318_21	euxassay_005318_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7662_wholemount_strong.wlz

7662_wholemount_moderate.wlz

7662_wholemount_weak.wlz

7662_wholemount_possible.wlz

7662_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7662_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8943
Entity Detected:	Ywhaz, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide ( MGI:109484)
Sequence:	sense strand is shown >T8943 GGTGTGGACGCCGAGCTCGCGACTGGAAAGGCAGGGCGTCATTCAGCCTCGCGCTTTTCCCAGCCTTAAA AGGTCTAAGGCCGCTTCCTGCAGCCAGAAGCGTTTGACGTCATCGTGCGTGTGGTGCCCCTGCTGCCGGG GCTAGTGATTGGAGGAAACCCCGTGTCTGCGGAGCGGCTGTAGCCTGTGAGCAGCCAGATCAGGGACAGA GTCTCAGCCTCGCCGCCACCGCTGCTGCCGCCGCCGCCCAGAGACCGCCGAGCCGCGTCCGTGTGCCGCC ACCCACTCCGGACACAGAATATCCAGTTATGGATAAAAATGAGCTGGTGCAGAAGGCCAAGCTGGCCGAG CAGGCAGAGCGATATGATGACATGGCAGCCTGCATGAAGTCTGTCACTGAGCAGGGAGCTGAGCTGTCGA ATGAGGAGAGAAACCTTCTCTCTGTTGCTTATAAAAACGTTGTAGGAGCCCGTAGGTCATCGTGGAGGGT CGTCTCAAGTATTGAGCAGAAGACGGAAGGTGCTGAGAAAAAGCAGCAGATGGCTCGAGAATACAGAGAG AAGATCGAGACGGAGCTGCGTGACATCTGCAACGATGTACTGTCTCTTTTGGAAAAGTTCTTGATCCCCA ATGCTTCGCAACCAGAAAGCAAAGTCTTCTATTTGAAAATGAAGGGTGACTACTACCGTTACTTGGCCGA GGTTGCTGCTGGTGATGACAAGAAAGGAATTGTGGACCAGTCACAGCAAGCATACCAAGAAGCATTTGAA ATCAGCAAAAAGGAGATGCAGCCGACACACCCCATCAGACTGGGTCTGGCCCTCAACTTCTCTGTGTTCT ATTACGAGATCCTGAACTCCCCAGAGAAAGCCTGCTCTCTTGCAAAAACAGCTTTCGATGAAGCCATTGC TGAACTTGATACATTAAGTGAAGAGTCGTACAAAGACAGCACGCTAATAATGCAGTTACTGAGAGACAAC TTAACATTGTGGACATCGGATACCCAAGGAGATGAAGCAGAAGCAGGAGAAGGAGGGGAAAATTAACCGG CCTTCCAACCTTTGTCTGCCTCATTCTAAAATTTACACAGTAGACCATTTGTCATCCATGCTGTCCCACA GATAGTTTTTTTGTTTACGATTTATGACAGGTTTATGTTACTTCTATTTGAATTTCTATATTTCCCATGT GGTTTTTATGTTTTAATATTAGGGGAGTAGAGCCAGTTAACTTTAGGGAGTTACTCATTTTCATCTTGAG GTGGCCAATATGGGATGTGGAATTTTTACATGAGTTACACATGTTTGGCATAGTACTTTTGGTACATTGT GGCTTCAGAAGGGCCAGTGTTAAAACTGCTTCCATGTCTAAGCAAAGAAAACTGCCTACATATTGGTGTG TGCTGGCGGGGAATAATCAGGATAATGGGTTCCAGTCATGAGTGTAGTCTTTGTGGGTACTGTAAGGCCT GGAGCACTTGTGAGGCTGTGACACAAACACCCTGTGGATGCACGCTAGGATCGTGTGTGTCTGCGTGCAC ACTCTTGACCACAGCTCCAGAAGTTGTCTCTAGACAAAGTCGTGACCCAATTTACTCTGGTAAGGGCAGA AACGGTTCACATTCCATTATTTGTAAAGTTACCTGCTGTTTGCTTTCATTATTTTTGCTACACATTTTAT TTGTATTTAAATGTTTTAGGCAATCTAAGAACAAATGTAAAAGTAAAGATGCAGTAAAAACGAGTTGCTT GGTGTGCCCGGCTCCATACGTATCAAGCACAGCGGTAAACAAAATCCCATGTATTTAACTTTTTCTTTCT TTTTTTTTTTTTTTTAAAGTTTTTTGTTTTGTTTTGTTTTTGCTTTTGTGATTTTTTTTTTTTCTTTTTG ATACTTGCCTAACATGCATGTGCTGTAAAAATAGTTAACAGGGAAATAACTTGAGATGACGGCTAGCTTT GTTTTAATGTCTTAAGAAATTTTCATGAACAATCCAAGCATAATTGTTAAGAACACATGTATTAAAGTTC ATGTAAGTGGAATAAAAGTTTTATGAATGGACTTTTCAACTACTTTTCTCTACAGCTTTTCATGTAAATT AGTCTTTTGGTTTTGAGACTTCTCCAAAGGAAATTGTCTCCTTATTCCCTCTTGGCAGCTAATGGGCTTT TACCAATTTTGAATGCAGAGTTTATCATAAAAAAAAAAAAACCGCTAATGTAGCTTCCATCCTCAGCCAC CTCCCACATTTTCCCCTTACTCCTCAACAATTGAAGTGGACTTTTATGGGGTGGGGGGTGATTGGCAAAA GGTAATGTGTTCCATTTATAATTTTGGTCTATGGAGTTTTCTAACTTAGGAAGCCACAATGTTCTTGGCC CATGTGACTGACACTGGGCAGCATTAACTGTTAAGTTCTGTGCTTCCAAGTCGCTGTTGGTTTTAAGAAT TTCCTGATGCTCTTACAGTCTGCCTGGGATTCTATTCCTCTATTTCCATGTTGGGCACAGGCTCACTTCC CTTTTACTGTCTTGTCACCAACCATTCCAACTTGGTGGCCATGTATTTGGGAAAAGGGCCGCATGATCTT TCTGGCTCCACTCAGTGTCTAAGATACCCGCTTCCCCTGCTTGCCTCCCACAGCCTCCCCTCATCCTCTC TACAGCCCAGCTCGCCTCAGTTGAGCTTGTGTTTATCTCCCTGGAAGTCCTGCCCTAAATGGTCTGTCAC CGTCTCCCTTTAAAATCCTTCCTCCTCCTCTTCCTCCTCCTCCTCCTCCTCACATAATGATGGGGCTAAG TCACACCCAAAGCTCACACCCTACCGAGTATTTCCTCAGTACTTTACAGAAAACACCAAACAAAAATGCC ATTTTACAAGAGGTGTATTTTTTTTTCTTTTAGAATGTAAGCTCGTCTAGAGCAGGGACAATGTTTTCTG TATGTTCTATTGTGCCTAGTACACTGTAAATGCTCAATAAATATTGATGATGGGAAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6419584 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6419584 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7663 same embryo
	EMAGE:7664 same embryo
	EMAGE:7665 same embryo
	EurExpress:euxassay_005318 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source