EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7680

Ogdh oxoglutarate dehydrogenase (lipoamide) ( MGI:1098267)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005288. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005288_01	euxassay_005288_02	euxassay_005288_03	euxassay_005288_04

euxassay_005288_05	euxassay_005288_06	euxassay_005288_07	euxassay_005288_08	euxassay_005288_09

euxassay_005288_10	euxassay_005288_11	euxassay_005288_12	euxassay_005288_13	euxassay_005288_14

euxassay_005288_15	euxassay_005288_16	euxassay_005288_17	euxassay_005288_18	euxassay_005288_19

euxassay_005288_20	euxassay_005288_21	euxassay_005288_22	euxassay_005288_23	euxassay_005288_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7680_wholemount_strong.wlz

7680_wholemount_moderate.wlz

7680_wholemount_weak.wlz

7680_wholemount_possible.wlz

7680_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7680_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8920
Entity Detected:	Ogdh, oxoglutarate dehydrogenase (lipoamide) ( MGI:1098267)
Sequence:	sense strand is shown >T8920 GAGGGTGGAGTTTTAGATCCTTGACGTCAAAAGGTCACTCAAGGAACACCTCTACAGGCCCAAGTGGGGT CAGTGGGGTCAACCTGTATTCGATGGAGGTCTCTACGTCCGTACTTGTGGAAGGCTTCAGGACAAAATGT TTCATTTAAGGACTTGTGCTGCTAAGTTAAGGCCATTGACAGCCTCCCAGACTGTTAAGACATTTTCACA AAACAAACCAGCAGCAATTAGGACGTTTCAACAGATTCGGTGCTATTCTGCACCTGTAGCTGCTGAACCA TTTCTTAGTGGGACTAGTTCGAACTATGTGGAGGAAATGTACTGTGCCTGGTTGGAGAATCCCAAAAGTG TACATAAGTCATGGGACATTTTTTTCCGAAACACCAATGCTGGAGCCCCACCGGGCACTGCCTACCAGAG CCCCCTTTCCCTGAGTCGAAGCTCCCTGGCTACCATGGCCCATGCACAGTCCCTGGTGGAAGCACAACCT AACGTCGACAAACTCGTGGAGGACCACTTGGCGGTGCAGTCTCTCATCAGGGCATATCAGGTCAGGGGTC ACCACATTGCAAAACTTGATCCTCTCGGAATTAGTTGTGTAAATTTTGATGATGCTCCGGTAACTGTTTC TTCAAACGTGGGGTTCTATGGCCTACACGAGTCTGACCTTGACAAGGTCTTCCACTTACCCACCACCACT TTCATCGGGGGACAGGAGCCAGCACTTCCTCTTCGGGAGATCATCCGTCGGCTGGAGATGGCCTACTGCC AGCACATTGGTGTGGAGTTCATGTTCATTAATGATTTGGAACAATGCCAGTGGATCCGACAGAAGTTTGA GACCCCTGGAATCATGCAGTTCACCAATGAGGAGAAGCGGACCTTGCTGGCCAGGCTTGTACGATCCACC AGGTTTGAGGAGTTCCTACAGCGAAAGTGGTCCTCGGAGAAGCGTTTTGGTCTGGAAGGCTGTGAGGTGC TGATCCCTGCCCTCAAGACAATCATTGATATGTCAAGTGCAAATGGAGTGGACTATGTGATCATGGGGAT GCCACACAGAGGACGGCTGAACGTGCTTGCAAATGTCATCAGGAAGGAGCTGGAGCAAATATTCTGTCAG TTTGACTCAAAGCTGGAGGCAGCTGATGAGGGTTCTGGGGACATGAAGTACCACCTGGGCATGTATCACC GCAGGATCAACCGTGTGACCGACAGAAACATCACTTTGTCCTTGGTGGCTAACCCTTCCCATCTAGAGGC TGCTGACCCTGTCGTGATGGGAAAGACCAAAGCTGAACAGTTCTACTGTGGAGACACTGAAGGGAAAAAG GTGATGTCTATCCTGCTGCATGGGGATGCTGCTTTTGCTGTCCAGGGCATCGTATATGAGACCTTCCATC TCAGCGACTTGCCGTCCTACACAACCCATGGCACTGTTCATGTGGTTGTCAACAACCAGATTGGCTTCAC CACAGACCCTCGGATGGCCCGCTCCTCTCCCTACCCCACTGATGTGGCCCGAGTGGTGAATGCCCCCATT TTCCATGTCAACTCAGATGACCCTGAAGCTGTCATGTATGTATGCAAGGTGGCAGCTGAGTGGAGAAACA CCTTCCACAAGGATGTTGTAGTTGATCTGGTGTGTTATCGACGAAATGGCCACAATGAGATGGACGAACC TATGTTTACACAGCCACTCATGTACAAGCAGATCCGCAAGCAGAAGCCTGTACTGCAGAAGTATGCAGAA TTGCTAGTCTCCCAGGGTTTCGTCAATCAGCCTGAGTACGAGGAGGAAATCTCCAAGTATGATAAGATCT GTGAGGAAGCATTTACCAGATCCAAAGATGAGAAGATCTTGCACATCAAGCACTGGCTGGATTCCCCCTG GCCTGGCTTTTTCACCCTGGATGGACAGCCCAGGAGCATGACCTGCCCCTCCACTGGCCTGGAGGAGGAT GTCTTGTTCCACATTGGAAAGGTGGCCAGCTCTGTACCTGTGGAGAACTTTACTATCCATGGAGGGCTGA GCCGGATCTTGAAGACCCGCAGAGAGCTTGTGACGAACCGGACTGTGGACTGGGCCCTGGCAGAGTACAT GGCATTTGGCTCACTGCTGAAGGAAGGCATCCATGTGCGGCTGAGTGGCCAGGATGTGGAGCGGGGCACC TTCAGCCATCGCCACCATGTGCTCCATGATCAGAATGTTGACAAAAGAACCTGCATCCCCATGAACCACC TTTGGCCAAATCAGGCCCCTTACACTGTATGCAACAGCTCGCTGTCTGAGTACGGTGTCCTGGGCTTTGA GCTGGGCTTTGCCATGGCTAGCCCTAATGCTCTGGTTCTCTGGGAGGCCCAGTTTGGTGACTTCAACAAC ATGGCACAGTGCATCATTGACCAGTTCATCTGCCCAGGACAGGCAAAGTGGGTGCGGCAGAATGGCATTG TGCTCCTGCTGCCTCATGGCATGGAAGGCATGGGTCCCGAGCATTCCTCTGCCCGCCCAGAGCGGTTTCT GCAGATGTGCAATGATGACCCAGATGTCCTGCCTGACCTGCAGGAAGAAAACTTTGACATCAATCAGCTC TATGACTGCAACTGGATTGTTGTCAACTGTTCCACCCCTGGCAACTTCTTCCATGTGCTGCGACGACAGA TCTTGCTGCCCTTCCGGAAGCCGTTAATCGTCTTCACTCCCAAATCCCTCCTGCGCCACCCTGAGGCAAG AACTAGCTTTGACGAGATGCTGCCAGGAACGCACTTCCAGCGTGTGATCCCAGAAAATGGACCTGCAGCT CAGGACCCACACAAAGTCAAGAGACTTCTCTTCTGCACTGGGAAGGTGTACTATGACCTCACCCGAGAGC GCAAAGCCAGGAACATGGAGGAGGAGGTGGCTATTACAAGGATTGAGCAGCTATCACCATTCCCCTTTGA CCTCCTGCTGAAAGAGGCACAGAAGTATCCCAATGCTGAGCTGGCCTGGTGCCAGGAAGAGCACAAGAAC CAAGGCTACTATGACTATGTCAAGCCAAGACTTCGTACCACCATTGACCGTGCTAAGCCTGTCTGGTATG CTGGCCGAGACCCGGCAGCTGCTCCAGCCACTGGCAACAAGAAAACACACCTGACAGAGCTGCAGCGCTT TCTGGACACAGCCTTTGACCTGGACGCATTCAAGAAATTCTCTTAGATGCTCCTGGAGTTGATGAGGCCA TGGCCCCCATGTCCATGACGCTCTTTGCTTCTCAACTAAAGAATAGTGCCTCAGCACTGTCCACACGTCC CTTCGCTGTGCCACACCACCCCTGTTCTCATAGGAATTAAGTTGTCCACTGCAGTGCTCAGCTGCTCCCC GGTCACATGCTGCCCAGCCTGTGCCGACTTCTCTCAGGCTGCACACCGTTCATGGAGACCGGAAGGAGCA GAATAAGGAAAGGGCCCCTCTCAGGACATCCTAGAGAAGGAAGGCAGCTCTGGCCCCACCCATGCCCCCA GTGCAATCCTCCAGGGTAGGAACAGAACCCTATGTGGCTTCCCAGGGTACTAGCACTCAGCCCTCGTCAC CCATCAAGTCGCAGATTCAAGGCCAGGAGTAGTTTCATCTTGCTAGGGCCAAGCTGAGAGCTCATGGAGG AACTATAGCTGCCAGGATTTGGGAGTCATCAGGATGTTGTGTGAATAGAGATTGTCATGGGGTATTTAGA GGACTTTAGCAGTGATGTTAGTCTAGCCCTGCTACCCTTCTTGGGTTTGGGCTGTATGTGGGAAACTTAC CCCAGCTACCACGCCTGGAGAGCTTGGCTCTGAGTACGGCCCAGAAGCTCCATTGGCTCCCAACGCCAGG CACTGCTGCCTCTTGGTCCTGCTGCCTCTGCTCTCCTGACCCCTCCCCAGTCACTTCATTTTCTCTGTTG TTCCCTTGAACACACAGAAGCTGTTGACGAATTCTTTTTTTTGCTGTGCCAAGGCAGGTCAAAAGCAGAT CAGTGGATAAGAGCAAGTTGTCCCAAGGAGCCAGCTGTCCTTCCTCCCTCTTTTGACCTCCACTGGGACA CACCTGATTTATTTATTTTGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6417392 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6417392 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7679 same embryo
	EMAGE:7681 same embryo
	EMAGE:7678 same embryo
	EurExpress:euxassay_005288 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source